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Literature summary for 1.1.1.10 extracted from
- Characterization and gene cloning of L-xylulose reductase involved in L-arabinose catabolism from the pentose-fermenting fungus Rhizomucor pusillus (2017), Biosci. Biotechnol. Biochem., 81, 1612-1618 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lxr3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3) | Rhizomucor pusillus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 92% inhibition at 5 mM | Rhizomucor pusillus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.71 | - |
L-xylulose | pH 7.0, 25°C, native enzyme | Rhizomucor pusillus | |
| 13.2 | - |
L-xylulose | pH 7.0, 25°C, recombinant enzyme | Rhizomucor pusillus | |
| 225 | - |
xylitol | pH 9.6, 25°C, recombinant enzyme | Rhizomucor pusillus | |
| 253 | - |
xylitol | pH 9.6, 25°C, native enzyme | Rhizomucor pusillus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Rhizomucor pusillus | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Rhizomucor pusillus | |
| Mn2+ | activates | Rhizomucor pusillus | |
| Zn2+ | activates | Rhizomucor pusillus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 98000 | - |
gel filtration | Rhizomucor pusillus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylitol + NADP+ | Rhizomucor pusillus | - |
L-xylulose + NADPH + H+ | - |
r | |
| xylitol + NADP+ | Rhizomucor pusillus NBRC 4578 | - |
L-xylulose + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor pusillus | A0A0M4UR95 | - |
- |
| Rhizomucor pusillus NBRC 4578 | A0A0M4UR95 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from cell-free extract of mycelia by dialysis, ammonium sulfate fractionation, hydrophobic interaction chromatography, again dialysis, followed by hydroxyapatite chromatography, anion exchange chromatography, ultrafiltration, and gel filtration | Rhizomucor pusillus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Rhizomucor pusillus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 53.9 | - |
purified recombinant RpLXR enzyme, reduction of L-xylulose with NADPH as cofactor, pH 7.0, 25°C | Rhizomucor pusillus |
| 57.8 | - |
purified recombinant RpLXR enzyme, oxidation of xylitol with NADP+ as cofactor, pH 9.6, 25°C | Rhizomucor pusillus |
| 77.3 | - |
purified native RpLXR enzyme, oxidation of xylitol with NADP+ as cofactor, pH 9.6, 25°C | Rhizomucor pusillus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity in oxidation and reduction reactions, overview. No activity with D-mannitol, D-sorbitol, D-xylose, or D-fructose as substrates. D-Xylulose is a poor substrate. Enzyme RpLXR exhibits not only a L-xylulose reductase activity but also a strong dicarbonyl reductase activity | Rhizomucor pusillus | ? | - |
- |
|
| additional information | substrate specificity in oxidation and reduction reactions, overview. No activity with D-mannitol, D-sorbitol, D-xylose, or D-fructose as substrates. D-Xylulose is a poor substrate. Enzyme RpLXR exhibits not only a L-xylulose reductase activity but also a strong dicarbonyl reductase activity | Rhizomucor pusillus NBRC 4578 | ? | - |
- |
|
| xylitol + NADP+ | - |
Rhizomucor pusillus | L-xylulose + NADPH + H+ | - |
r | |
| xylitol + NADP+ | - |
Rhizomucor pusillus NBRC 4578 | L-xylulose + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 29000, SDS-PAGE, 4 * 28341, sequence calculation | Rhizomucor pusillus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-xylulose reductase | - |
Rhizomucor pusillus |
| LXR | - |
Rhizomucor pusillus |
| LXR3 | - |
Rhizomucor pusillus |
| RpLXR | - |
Rhizomucor pusillus |
| Rplxr3 | - |
Rhizomucor pusillus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rhizomucor pusillus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
reduction of L-xylulose | Rhizomucor pusillus |
| 9 | - |
oxidation of xylitol | Rhizomucor pusillus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity toward L-xylulose with NADH is 3.1% compared to NADPH | Rhizomucor pusillus | |
| NADP+ | - |
Rhizomucor pusillus | |
| NADPH | - |
Rhizomucor pusillus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Rhizomucor pusillus | transcription of the Rplxr3 gene in Rhizomucor pusillus strain NBRC 4578 is inhibited in the presence of D-glucose, D-xylose, and D-mannitol | down |
| Rhizomucor pusillus | transcription of the Rplxr3 gene in Rhizomucor pusillus strain NBRC 4578 is induced in the presence of L-arabinose | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the SDR superfamily | Rhizomucor pusillus |
| metabolism | enzyme RpLXR is involved in the L-arabinose catabolic pathway | Rhizomucor pusillus |
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