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EC Tree
IUBMB Comments The enzyme is involved in the biosynthetic pathway of dTDP-6-deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II.
The enzyme appears in viruses and cellular organisms
Synonyms
dTDP-4-keto-6-deoxy-D-glucose 3-epimerase, dTDP-4-keto-6-deoxyglucose 3-epimerase, dTDP-deoxyglucose 3-epimerase, GerF,
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dTDP-4-keto-6-deoxy-D-glucose 3-epimerase
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dTDP-4-keto-6-deoxyglucose 3-epimerase
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dTDP-deoxyglucose 3-epimerase
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GerF
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-gulose
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose 3-epimerase
The enzyme is involved in the biosynthetic pathway of dTDP-6-deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II.
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-6-deoxy-beta-D-gulose
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-6-deoxy-beta-D-gulose
the enzyme is involved in the mycinose biosynthetic pathway of several macrolide antibiotics
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-6-deoxy-beta-D-gulose
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dTDP-4-dehydro-6-deoxy-D-glucopyranose. The enzyme exhibits epimerase activity, as evidenced by the detection of maltol, the decomposition product of the activated intermediate
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-6-deoxy-beta-D-gulose
failures to acquire an isolation product from the GerF enzyme assay (detection of maltol, the decomposition product of the activated intermediate). However, the successful isolation of dTDP-6-deoxy-D-allose (in a coupled assay with GerKI) provides evidence sufficient to confirm that GerF functions as a dTDP-4-keto-6-deoxyglucose 3-epimerase in the mycinose biosynthesis pathway
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-6-deoxy-beta-D-gulose
the enzyme is involved in the mycinose biosynthetic pathway of several macrolide antibiotics
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brenda
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UniProt
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physiological function
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the enzyme is involved in the mycinose biosynthetic pathway of several macrolide antibiotics
physiological function
the enzyme is involved in the mycinose biosynthetic pathway of several macrolide antibiotics
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CHMJ_STRBI
196
0
21683
Swiss-Prot
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GERF_STRSQ
Streptomyces sp
196
0
21722
Swiss-Prot
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A0A1Y2NMM6_STRFR
225
0
24139
TrEMBL
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A0A7K0BTZ9_9ACTN
216
0
23573
TrEMBL
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A0A177HJ54_9ACTN
202
0
21842
TrEMBL
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A0A3N6GFQ7_9ACTN
203
0
22044
TrEMBL
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A0A3N6FE36_9ACTN
196
0
21722
TrEMBL
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A0A5P9PPC8_9PSEU
209
0
22457
TrEMBL
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A0A1D8G1X8_9ACTN
225
0
24153
TrEMBL
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38000
x * 38000, including the 6 His-tag and thioredoxin-tagged fusion protein, SDS-PAGE
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x * 38000, including the 6 His-tag and thioredoxin-tagged fusion protein, SDS-PAGE
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recombinant enzyme
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expression in Eschericha coli using recombinant expression vector pHJ3
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expression in Escherichia coli BL21
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Sohng, J.K.; Kim, H.J.; Nam, D.H.; Lim, D.O.; Han, J.M.; Lee, H.J.; Yoo, J.C.
Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155
Biotechnol. Lett.
26
185-191
2004
Streptomyces sp.
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Thuy, T.T.; Liou, K.; Oh, T.J.; Kim, D.H.; Nam, D.H.; Yoo, J.C.; Sohng, J.K.
Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP
Glycobiology
17
119-126
2007
Streptomyces sp. (Q331R1)
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