Literature summary for 5.1.3.27 extracted from

Thuy, T.T.; Liou, K.; Oh, T.J.; Kim, D.H.; Nam, D.H.; Yoo, J.C.; Sohng, J.K.
Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP (2007), Glycobiology, 17, 119-126.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	x * 38000, including the 6 His-tag and thioredoxin-tagged fusion protein, SDS-PAGE	Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dTDP-4-dehydro-6-deoxy-alpha-D-glucose	Streptomyces sp.	the enzyme is involved in the mycinose biosynthetic pathway of several macrolide antibiotics	dTDP-4-dehydro-6-deoxy-beta-D-gulose	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	Q331R1	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Streptomyces sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dTDP-4-dehydro-6-deoxy-alpha-D-glucose	the enzyme is involved in the mycinose biosynthetic pathway of several macrolide antibiotics	Streptomyces sp.	dTDP-4-dehydro-6-deoxy-beta-D-gulose	-	?
dTDP-4-dehydro-6-deoxy-alpha-D-glucose	failures to acquire an isolation product from the GerF enzyme assay (detection of maltol, the decomposition product of the activated intermediate). However, the successful isolation of dTDP-6-deoxy-D-allose (in a coupled assay with GerKI) provides evidence sufficient to confirm that GerF functions as a dTDP-4-keto-6-deoxyglucose 3-epimerase in the mycinose biosynthesis pathway	Streptomyces sp.	dTDP-4-dehydro-6-deoxy-beta-D-gulose	-	?

Subunits

Subunits	Comment	Organism
?	x * 38000, including the 6 His-tag and thioredoxin-tagged fusion protein, SDS-PAGE	Streptomyces sp.

Synonyms

Synonyms	Comment	Organism
dTDP-4-keto-6-deoxyglucose 3-epimerase	-	Streptomyces sp.
GerF	-	Streptomyces sp.

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in the mycinose biosynthetic pathway of several macrolide antibiotics	Streptomyces sp.

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Release 2023.1 (January 2023)