exchange of sequences corresponding by homology to the C-terminal domain including the domain spanning alpha-helix, between sabinene synthase and both bornyl diphosphate synthase and cineole synthase. Exchange of tresidues 304–377 from cineole synthase into sabinene synthase is sufficient to impart the alternative termination chemistry involving water capture to alpha-terpineol and cyclization to 1,8-cineole
strategies in engineering sabinene production, overview. Biosynthetic pathway using both the non-mevalonate MEP and mevalonate MVA pathways combining the geranyl diphosphate synthase genes and sabinene synthase from Salvia pomifera, and engineering native Erg20p into a GPP synthase and heterologous expression of sabinene synthase from Salvia pomifera
the foliar application of gibberellic acid increases gene expression of the monoterpene synthases sabinene synthase, borneol synthase, and 1,8-cineole synthase. The amounts of alpha- and beta-thujene are not transcriptionally regulated
Wise, M.L.; Savage, T.J.; Katahira, E.; Croteau, R.
Monoterpene synthases from common sage (Salvia officinalis). cDNA isolation, characterization, and functional expression of (+)-sabinene synthase, 1,8-cineole synthase, and (+)-bornyl diphosphate synthase