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IUBMB Comments Does not act on alginic acid, which is a copolymer of polymannuronate.
3.2.1.121
wiley-liss
template-based
server
seventh
scoring
Synonyms
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polymannuronate depolymerase
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polymannuronic acid polymerase
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R1-(ManAbeta(1-4))7-R2 + H2O = R1-(ManAbeta(1-4))5 + (ManAbeta(1-4))2-R2
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hydrolysis of O-glycosyl bond
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poly(mannuronide) mannuronohydrolase
Does not act on alginic acid, which is a copolymer of polymannuronate.
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alginic acid + H2O
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Substrates: little activity
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polymannuronic acid exopolysaccharide + H2O
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Substrates: from Pseudomonas aeruginosa, strain PM1
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polymannuronic acid exopolysaccharide + H2O
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Substrates: from Pseudomonas aeruginosa, strain PM1
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CaCl2
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5 mM: slight stimulation, at 17 mM inhibition
KCl
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3 mM: stimulation, above 10 mM: inhibition
MgCl2
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3 mM: slight stimulation, at 9 mM inhibition
MgSO4
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3 mM: slight stimulation, at 14 mM inhibition
NaCl
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3 mM: stimulation, above 10 mM: inhibition
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CaCl2
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at 17 mM inhibition
EDTA
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0.5 mM: 35% inhibition
KCl
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50 mM: 50% inhibition
MgCl2
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at 9 mM inhibition
MgSO4
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at 14 mM inhibition
NaCl
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50 mM: 40% inhibition
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0.096
polymannuronic acid exopolysaccharide
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37°C, pH 7.5, from Pseudomonas aeruginosa, strain PM1
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0.8
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sonicated cells with EDTA
2
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sonicated cells without EDTA
3.8
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supernatant of cells with EDTA
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brenda
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brenda
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associated with outer cell membrane
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Highest Expressing Human Cell Lines
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additional information
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major band: 53000 Da, minor band: 23000 Da, SDS-PAGE
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Dunne, M.W.; Buckmire, F.L.A.
Partial purification and characterization of a polymannuronic acid depolymerase produced by a mycoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis
Appl. Environ. Microbiol.
50
562-567
1985
Pseudomonas aeruginosa
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