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Information on EC 2.7.1.221 - N-acetylmuramate 1-kinase
for references in articles please use BRENDA:EC2.7.1.221
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EC Tree 2 Transferases
2.7 Transferring phosphorus-containing groups
2.7.1 Phosphotransferases with an alcohol group as acceptor
2.7.1.221 N-acetylmuramate 1-kinase
IUBMB Comments
The enzyme, characterized from Pseudomonas species, participates in a peptidoglycan salvage pathway.
The enzyme appears in viruses and cellular organisms
showSynonyms
murnac/glcnac anomeric kinase, murnac/glcnac kinase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
amgK
MurNAc/GlcNAc kinase
N-acetylmuramate/N-acetylglucosamine kinase
PA0596
pp_0405
amgK
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + N-acetyl-D-muramate = ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
peptidoglycan recycling II
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SYSTEMATIC NAME
IUBMB Comments
ATP:N-acetyl-D-muramate 1-phosphotransferase
The enzyme, characterized from Pseudomonas species, participates in a peptidoglycan salvage pathway.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + ((2R)-2-(((3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl)oxy)propanoyl)-L-alanine
ADP + ((2R)-2-(((3R,4R,5S,6R)-3-acetamido-5-hydroxy-6-(hydroxymethyl)-2-(phosphonooxy)tetrahydro-2H-pyran-4-yl)oxy)propanoyl)-L-alanine
-
Substrates: -
Products: -
Products: -
?
ATP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
ADP + 2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
ADP + 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
ADP + 2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
ADP + 2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 2-[3-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]propanamido]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
ADP + 2-[3-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]propanamido]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(2,2-difluoroacetamido)-D-glucopyranose
ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(2,2-difluoroacetamido)-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(3-[[2-([5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl]amino)ethyl]disulfanyl]propanamido)-D-glucopyranose
ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-(3-[[2-([5-[(3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl]amino)ethyl]disulfanyl]propanamido)-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-([5-[(3aR,6R,6aS)-2-oxohexahydro-1H-thieno[2,3-d]imidazol-6-yl]pentanoyl]amino)-D-glucopyranose
ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-([5-[(3aR,6R,6aS)-2-oxohexahydro-1H-thieno[2,3-d]imidazol-6-yl]pentanoyl]amino)-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[(4-oxopentanoyl)amino]-D-glucopyranose
ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[(4-oxopentanoyl)amino]-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[2-[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]acetamido]-D-glucopyranose
ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[2-[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]acetamido]-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[3-(3-methyl-3H-diazirin-3-yl)propanamido]-D-glucopyranose
ADP + 3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[3-(3-methyl-3H-diazirin-3-yl)propanamido]-1-O-phosphono-D-glucopyranose
-
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-D-galactosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-D-galactosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine
Products: -
Products: -
?
additional information
?
-
Substrates: no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-D-galactosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-D-galactosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
Substrates: -
Products: -
Products: -
?
ATP + N-acetyl-D-muramate
ADP + N-acetyl-alpha-D-muramate 1-phosphate
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine
Products: -
Products: -
?
additional information
?
-
Substrates: no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0304
2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
0.0508
ATP
pH not specified in the publication, temperature not specified in the publication
0.177
N-acetyl-D-glucosamine
pH not specified in the publication, temperature not specified in the publication
0.08
N-acetyl-D-muramate
pH not specified in the publication, temperature not specified in the publication
1.12
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
1.12
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
0.0303
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
0.0303
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
0.55
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
0.55
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6.58
2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
4.38
ATP
pH not specified in the publication, temperature not specified in the publication
6.64
N-acetyl-D-glucosamine
pH not specified in the publication, temperature not specified in the publication
5.15
N-acetyl-D-muramate
pH not specified in the publication, temperature not specified in the publication
14.07
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
14.07
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
4.67
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
4.67
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
9.74
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
9.74
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
216
2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
1.02
2-[3-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]propanamido]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
2.95
3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-([5-[(3aR,6R,6aS)-2-oxohexahydro-1H-thieno[2,3-d]imidazol-6-yl]pentanoyl]amino)-D-glucopyranose
-
at pH 7.9 and 25°C
3.46
3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[(4-oxopentanoyl)amino]-D-glucopyranose
-
at pH 7.9 and 25°C
2.25
3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[2-[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]acetamido]-D-glucopyranose
-
at pH 7.9 and 25°C
1.55
3-O-[(1R)-1-carboxyethyl]-2-deoxy-2-[3-(3-methyl-3H-diazirin-3-yl)propanamido]-D-glucopyranose
-
at pH 7.9 and 25°C
12.6
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
12.6
2-(2-azidoacetamido)-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
154
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
154
2-acetamido-3-O-[(1R)-2-azido-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
18
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
pH and temperature not specified in the publication
18
2-[(but-3-ynoyl)amino]-3-O-[(1R)-1-carboxyethyl]-2-deoxy-D-glucopyranose
-
at pH 7.9 and 25°C
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
malfunction
-
the deletion of the enzyme results in reduced resistance against fosfomycin
malfunction
-
the deletion of the enzyme results in reduced resistance against fosfomycin
-
physiological function
enzyme is involved in a cell wall sugar-recycling route that channels external MurNAc directly to peptidoglycan biosynthesis. A deletion mutant shows increase fosfomycin sensitivity
physiological function
-
MurNAc accumulates in the cell extract of AmgK mutants. Mutant is not impaired in growth in rich medium. Mutant displays a fourfold increase in fosfomycin susceptibility
physiological function
-
enzyme is involved in a cell wall sugar-recycling route that channels external MurNAc directly to peptidoglycan biosynthesis. A deletion mutant shows increase fosfomycin sensitivity
-
physiological function
-
MurNAc accumulates in the cell extract of AmgK mutants. Mutant is not impaired in growth in rich medium. Mutant displays a fourfold increase in fosfomycin susceptibility
-
Show AA Sequence and Transmembrane Helices (175 entries)
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione Sepharose column chromatography and Superdex S200 gel filtration
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Borisova, M.; Gisin, J.; Mayer, C.
Blocking peptidoglycan recycling in Pseudomonas aeruginosa attenuates intrinsic resistance to fosfomycin
Microb. Drug Resist.
20
231-237
2014
Pseudomonas aeruginosa, Pseudomonas aeruginosa PA01
brenda
Gisin, J.; Schneider, A.; Naegele, B.; Borisova, M.; Mayer, C.
A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis
Nat. Chem. Biol.
9
491-493
2013
Pseudomonas putida (Q88QT3), Pseudomonas putida DSM 6125 (Q88QT3)
brenda
Sonnabend, M.S.; Klein, K.; Beier, S.; Angelov, A.; Kluj, R.; Mayer, C.; Gross, C.; Hofmeister, K.; Beuttner, A.; Willmann, M.; Peter, S.; Oberhettinger, P.; Schmidt, A.; Autenrieth, I.B.; Schuetz, M.; Bohn, E.
Identification of drug resistance determinants in a clinical isolate of Pseudomonas aeruginosa by high-density transposon mutagenesis
Antimicrob. Agents Chemother.
64
e01771-19
2020
Pseudomonas aeruginosa, Pseudomonas aeruginosa ID40
brenda
DeMeester, K.E.; Liang, H.; Jensen, M.R.; Jones, Z.S.; D'Ambrosio, E.A.; Scinto, S.L.; Zhou, J.; Grimes, C.L.
Synthesis of functionalized N-acetyl muramic acids to probe bacterial cell wall recycling and biosynthesis
J. Am. Chem. Soc.
140
9458-9465
2018
Pseudomonas putida
brenda
Ukaegbu, O.I.; DeMeester, K.E.; Liang, H.; Brown, A.R.; Jones, Z.S.; Grimes, C.L.
Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates
Methods Enzymol.
638
1-26
2020
Pseudomonas putida
brenda
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