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Literature summary for 2.7.1.221 extracted from

  • Gisin, J.; Schneider, A.; Naegele, B.; Borisova, M.; Mayer, C.
    A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis (2013), Nat. Chem. Biol., 9, 491-493 .
    View publication on PubMed
Search for more literature for 2.7.1.221

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0508
-
 ATP pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
0.08
-
 N-acetyl-D-muramate pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
0.177
-
 N-acetyl-D-glucosamine pH not specified in the publication, temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-galactosamine Pseudomonas putida
-
 ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-galactosamine Pseudomonas putida DSM 6125
-
 ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-glucosamine Pseudomonas putida
-
 ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-glucosamine Pseudomonas putida DSM 6125
-
 ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-muramate Pseudomonas putida
-
 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
ATP + N-acetyl-D-muramate Pseudomonas putida DSM 6125
-
 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
additional information Pseudomonas putida no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine ?
-
 ?
additional information Pseudomonas putida DSM 6125 no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88QT3 member of protein family PF01636
-
Pseudomonas putida DSM 6125 Q88QT3 member of protein family PF01636
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-galactosamine
-
 Pseudomonas putida ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-galactosamine
-
 Pseudomonas putida DSM 6125 ADP + N-acetyl-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-glucosamine
-
 Pseudomonas putida ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-glucosamine
-
 Pseudomonas putida DSM 6125 ADP + N-acetyl-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-muramate
-
 Pseudomonas putida ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
ATP + N-acetyl-D-muramate
-
 Pseudomonas putida DSM 6125 ADP + N-acetyl-alpha-D-muramate 1-phosphate
-
 ?
additional information no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine Pseudomonas putida ?
-
 ?
additional information no substrates: N-acetyl-D-mannosamine, N-deacetylated sugars or glucosamine Pseudomonas putida DSM 6125 ?
-
 ?

Synonyms

Synonyms Comment Organism
amgK
-
 Pseudomonas putida
pp_0405
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.38
-
 ATP pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
5.15
-
 N-acetyl-D-muramate pH not specified in the publication, temperature not specified in the publication Pseudomonas putida
6.64
-
 N-acetyl-D-glucosamine pH not specified in the publication, temperature not specified in the publication Pseudomonas putida

General Information

General Information Comment Organism
physiological function enzyme is involved in a cell wall sugar-recycling route that channels external MurNAc directly to peptidoglycan biosynthesis. A deletion mutant shows increase fosfomycin sensitivity Pseudomonas putida