Information on EC 2.6.1.93 - neamine transaminase

for references in articles please use BRENDA:EC2.6.1.93
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.6.1.93
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RECOMMENDED NAME
GeneOntology No.
neamine transaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
neamine + 2-oxoglutarate = 6'-dehydroparomamine + L-glutamate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
kanamycin biosynthesis
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ribostamycin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
neamine:2-oxoglutarate aminotransferase
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin B, butirosin, neomycin and ribostamycin. Works in combination with EC 1.1.3.43, paromamine 6-oxidase, to replace the 6'-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces kanamyceticus can also catalyse EC 2.6.1.94, 2'-deamino-2'-hydroxyneamine transaminase, which leads to production of kanamycin A [3]. The enzyme from the bacterium Streptomyces fradiae can also catalyse EC 2.6.1.95, leading to production of neomycin C [2].
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6'-dehydroparomamine + L-glutamate
neamine + 2-oxoglutarate
show the reaction diagram
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PDB
SCOP
CATH
UNIPROT
ORGANISM
Streptomyces fradiae;
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48060
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Neo-18, calculated from amino acid sequence; Neo-18, electrospray ionization mass spectrometry
52070
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BtrB, calculated from amino acid sequence; BtrB, electrospray ionization mass spectrometry
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA resin column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expressed in Streptomyces venezuelae strain PAR
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LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.93)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)