Information on EC 2.5.1.102 - geranyl-pyrophosphate-olivetolic acid geranyltransferase

for references in articles please use BRENDA:EC2.5.1.102

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EC Tree 2 Transferases 2.5 Transferring alkyl or aryl groups, other than methyl groups 2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date) 2.5.1.102 geranyl-pyrophosphate-olivetolic acid geranyltransferase

IUBMB Comments

Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also use neryl diphosphate as substrate, forming cannabinerolate.

The expected taxonomic range for this enzyme is: Cannabis sativa

Reaction Schemes

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geranyl diphosphate

2,4-dihydroxy-6-pentylbenzoate

diphosphate

cannabigerolate

Synonyms
geranylpyrophosphate:olivetolate geranyltransferase, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

geranylpyrophosphate:olivetolate geranyltransferase

GOT

PT4-T

geranylpyrophosphate:olivetolate geranyltransferase

Cannabis sativa

719479

geranylpyrophosphate:olivetolate geranyltransferase

GOT

GOT

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

geranyl diphosphate + 2,4-dihydroxy-6-pentylbenzoate = diphosphate + cannabigerolate

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Go to Pathway Search

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PATHWAY SOURCE

PATHWAYS

KEGG

Biosynthesis of secondary metabolites, Biosynthesis of various secondary metabolites - part 1

MetaCyc

cannabinoid biosynthesis

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SYSTEMATIC NAME

IUBMB Comments

geranyl-diphosphate:olivetolate geranyltransferase

Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also use neryl diphosphate as substrate, forming cannabinerolate.

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

geranyl diphosphate + 2,4-dihydroxy-6-pentylbenzoate

diphosphate + cannabigerolate

2 entries

geranyl diphosphate + olivetolic acid

diphosphate + cannabigerolate

Cannabis sativa

A0A455ZJC3

759845

Substrates: -
Products: -

additional information

Cannabis sativa

719479

Substrates: the enzyme accepts geranyldiphosphate and to a lesser degree also neryldiphosphate as a cosubstrate. Olivetol is inactive as a prenyl acceptor, GOT is specific for olivetolic acid
Products: -

geranyl diphosphate + 2,4-dihydroxy-6-pentylbenzoate

diphosphate + cannabigerolate

Cannabis sativa

719479

Substrates: -
Products: -

geranyl diphosphate + 2,4-dihydroxy-6-pentylbenzoate

diphosphate + cannabigerolate

Cannabis sativa

719479

Substrates: i.e. olivetolate
Products: -

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

geranyl diphosphate + 2,4-dihydroxy-6-pentylbenzoate

diphosphate + cannabigerolate

Cannabis sativa

719479

Substrates: -
Products: -

geranyl diphosphate + olivetolic acid

diphosphate + cannabigerolate

Cannabis sativa

A0A455ZJC3

759845

Substrates: -
Products: -

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mg2+

Cannabis sativa

essentially required

719479

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.00673

olivetolic acid

Cannabis sativa

A0A455ZJC3

at pH 8.5 and 30°C

759845

additional information

Cannabis sativa

Michaelis-Menten kinetics with geranyl diphosphate as substrate

719479

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Cannabis sativa

719479

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Cannabis sativa

719479

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

UniProt

brenda

Cannabis sativa

var. Skunk

719479

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

young

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

metabolism

Cannabis sativa

GOT is the first enzyme in the biosynthesis of DELTA1-tetrahydrocannabinolic acid and cannabinoids

719479

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Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

A0A455ZJC3 pBLAST

A0A455ZJC3_CANSA

Cannabis sativa

398

44928

TrEMBL

Show Sequence

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in yeast

Cannabis sativa

A0A455ZJC3

759845

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

719479

Fellermeier, M.; Zenk, M.

Prenylation of olivetolate by a hemp transferase yields cannabigerolic acid, the precursor of tetrahydrocannabinol

FEBS Lett.

427

283-285

1998

Cannabis sativa

9607329

brenda

759845

Luo, X.; Reiter, M.; d'Espaux, L.; Wong, J.; Denby, C.; Lechner, A.; Zhang, Y.; Grzybowski, A.; Harth, S.; Lin, W.; Lee, H.; Yu, C.; Shin, J.; Deng, K.; Benites, V.; Wang, G.; Baidoo, E.; Chen, Y.; Dev, I.; Petzold, C.; Keasling, J.

Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

Nature

567

123-126

2019

Cannabis sativa (A0A455ZJC3), Cannabis sativa

30814733

brenda

Entries 1 - 20 of 2

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EXTERNAL LINKS (specific for EC-Number 2.5.1.102)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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