Any feedback?
No. of entries
Information on EC 2.4.1.247 - beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase
for references in articles please use BRENDA:EC2.4.1.247
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.247 beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase
IUBMB Comments
The enzyme from Clostridium phytofermentans is also active towards towards β-D-galactosyl derivatives of L-mannose, L-lyxose, D-glucose, 2-deoxy-D-glucose, and D-galactose in this order. Differs from 1,3-β-galactosyl-N-acetylhexosamine phosphorylase (EC 2.4.1.211) in being active towards L-rhamnose and inactive towards N-acetyl hexosamine derivatives.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
showSynonyms
cphy1920 protein, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
GalRhaP
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
beta-D-galactosyl-(1->4)-L-rhamnose + phosphate = L-rhamnose + alpha-D-galactose 1-phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
beta-D-galactosyl-(1->4)-L-rhamnose:phosphate 1-alpha-D-galactosyltransferase
The enzyme from Clostridium phytofermentans is also active towards towards beta-D-galactosyl derivatives of L-mannose, L-lyxose, D-glucose, 2-deoxy-D-glucose, and D-galactose in this order. Differs from 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase (EC 2.4.1.211) in being active towards L-rhamnose and inactive towards N-acetyl hexosamine derivatives.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-2-deoxy-D-glucose + phosphate
-
Substrates: reverse (synthetic) reaction
Products: -
Products: -
r
2-deoxy-D-glucose + alpha-D-galactose 1-phosphate
D-galactosyl-beta-1,3-2-deoxy-D-glucose + phosphate
Substrates: when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides
Products: -
Products: -
r
alpha-D-galactose 1-phosphate + L-rhamnose
D-galactosyl-beta-1,4-L-rhamnose + phosphate
Substrates: highest activity
Products: -
Products: -
r
beta-D-galactosyl-(1->3)-D-glucose + phosphate
D-glucose + alpha-D-galactose 1-phosphate
-
Substrates: lower activity
Products: -
Products: -
r
beta-D-galactosyl-(1->4)-L-rhamnose + phosphate
L-rhamnose + alpha-D-galactose 1-phosphate
D-galactose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-D-galactose + phosphate
-
Substrates: reverse (synthetic) reaction
Products: -
Products: -
r
D-galactose + alpha-D-galactose 1-phosphate
D-galactosyl-beta-1,3-D-galactose + phosphate
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-D-glucose + phosphate
-
Substrates: reverse (synthetic) reaction
Products: -
Products: -
r
D-glucose + alpha-D-galactose 1-phosphate
D-galactosyl-beta-1,3-D-glucose + phosphate
Substrates: when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides
Products: -
Products: -
r
D-xylose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-D-xylose + phosphate
-
Substrates: reverse (synthetic) reaction
Products: -
Products: -
r
L-mannose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-L-mannose + phosphate
-
Substrates: reverse (synthetic) reaction
Products: -
Products: -
r
L-mannose + alpha-D-galactose 1-phosphate
D-galactosyl-beta-1,4-L-mannose + phosphate
Substrates: -
Products: -
Products: -
r
L-rhamnose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-L-rhamnose + phosphate
-
Substrates: reverse (synthetic) reaction
Products: -
Products: -
r
beta-D-galactosyl-(1->4)-L-rhamnose + phosphate
L-rhamnose + alpha-D-galactose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
beta-D-galactosyl-(1->4)-L-rhamnose + phosphate
L-rhamnose + alpha-D-galactose 1-phosphate
-
Substrates: preferred substrate
Products: -
Products: -
r
L-lyxose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-L-lyxose + phosphate
Substrates: -
Products: -
Products: -
r
L-lyxose + alpha-D-galactose 1-phosphate
beta-D-galactosyl-(1->4)-L-lyxose + phosphate
-
Substrates: reverse (synthetic) reaction
Products: -
Products: -
r
additional information
?
-
Substrates: phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP
Products: -
Products: -
?
additional information
?
-
-
Substrates: phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP
Products: -
Products: -
?
additional information
?
-
-
Substrates: in the reverse reaction, the enzyme shows higher activity on L-rhamnose derivatives than on D-glucose derivatives, no activity with lactose. The enzyme only exhibits activity on Gal 1-phosphate as a donor and not on alpha-D-glucose 1-phosphate and alpha-N-acetyl-D-glucosamine 1-phosphate. Various acceptors in the presence of Gal 1-P act as a donor using a reverse (synthetic) reaction with highest activity on L-Rha.The enzyme also exhibits activity on derivatives of L-Rha, but Oter 1377 protein shows no or very poor activity on D-GlcNAc, D-GalNAc, D-Man, D-glucosamine, L-Glc, D-Fru, D-Rib, L-Fuc, L-Ara, D-Lyx, D-glucal, sucrose, or maltose, substrate specificity, overview
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-D-galactosyl-(1->4)-L-rhamnose + phosphate
L-rhamnose + alpha-D-galactose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
60
beta-D-galactosyl-(1->4)-L-rhamnose
-
recombinant enzyme, pH 7.0, 37°C, forward reaction
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.17
-
recombinant enzyme, substrate D-galactose, reverse reaction, pH 7.0, 37°C
0.37
-
recombinant enzyme, substrate D-xylose, reverse reaction, pH 7.0, 37°C
0.41
-
recombinant enzyme, substrate 2-deoxy-D-glucose, reverse reaction, pH 7.0, 37°C
0.62
-
recombinant enzyme, substrate D-glucose, reverse reaction, pH 7.0, 37°C
27
-
purified recombinant enzyme, substrate beta-D-galactosyl-(1->4)-L-rhamnose, forward reaction, pH 7.0, 37°C
29
-
recombinant enzyme, substrate L-rhamnose, reverse reaction, pH 7.0, 37°C
8.4
-
recombinant enzyme, substrate L-mannose, reverse reaction, pH 7.0, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 7
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bacterium possesses three GalHexNAcP homologous genes, cphy1920 gene; anaerobic cellulolytic bacterium
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
proteins are predicted not to possess N-terminal signal peptides
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
GalRhaPOt may be involved in rhamnogalacturonan I, in pectin, metabolism
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GLRP_LACP7
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
722
0
83279
Swiss-Prot
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
80000
84847
-
x * 80000, recombinant C-terminally His6-tagged Oter 1377 protein, SDS-PAGE, x * 84847, recombinant C-terminally His6-tagged Oter 1377 protein, sequence calculation
80000
-
x * 80000, recombinant C-terminally His6-tagged Oter 1377 protein, SDS-PAGE, x * 84847, recombinant C-terminally His6-tagged Oter 1377 protein, sequence calculation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 80000, recombinant C-terminally His6-tagged Oter 1377 protein, SDS-PAGE, x * 84847, recombinant C-terminally His6-tagged Oter 1377 protein, sequence calculation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
-
recombinant C-terminally His6-tagged Oter 1377 protein, 30 min, 90% activity remaining
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His6-tagged Oter 1377 protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic tree, cloning of the modified oter 1377 gene from plasmid pUC57 to pET-30a(+) and expression as C-terminally His6-tagged protein in Escherichia coli strain BL21(DE3)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
suitable catalyst for practical syntheses of oligosaccharides
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakajima, M.; Nishimoto, M.; Kitaoka, M.
Characterization of three beta-galactoside phosphorylases from clostridium phytofermentans: discovery of D-galactosyl-beta1,4-L-rhamnose phosphorylase
J. Biol. Chem.
284
19220-19227
2009
Lachnoclostridium phytofermentans (A9KHK4), Lachnoclostridium phytofermentans
brenda
Nakajima, M.; Nishimoto, M.; Kitaoka, M.
Characterization of D-galactosyl-beta1->4-L-rhamnose phosphorylase from Opitutus terrae
Enzyme Microb. Technol.
46
315-319
2010
Opitutus terrae
-
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.247)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
