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Literature summary for 2.4.1.247 extracted from
- Characterization of three beta-galactoside phosphorylases from clostridium phytofermentans: discovery of D-galactosyl-beta1,4-L-rhamnose phosphorylase (2009), J. Biol. Chem., 284, 19220-19227.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | suitable catalyst for practical syntheses of oligosaccharides | Lachnoclostridium phytofermentans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Lachnoclostridium phytofermentans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
L-rhamnose | - |
Lachnoclostridium phytofermentans |  |
| 7.9 | - |
D-galactosyl-beta-1,4-L-rhamnose | - |
Lachnoclostridium phytofermentans | |
| 78 | - |
L-Mannose | - |
Lachnoclostridium phytofermentans | |
| 220 | - |
L-Lyxose | - |
Lachnoclostridium phytofermentans | |
| 290 | - |
2-deoxy-D-glucose | - |
Lachnoclostridium phytofermentans | |
| 460 | - |
D-glucose | - |
Lachnoclostridium phytofermentans | |
| 610 | - |
D-galactose | - |
Lachnoclostridium phytofermentans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | proteins are predicted not to possess N-terminal signal peptides | Lachnoclostridium phytofermentans | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
SDS-PAGE | Lachnoclostridium phytofermentans |
| 84230 | - |
calculated | Lachnoclostridium phytofermentans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lachnoclostridium phytofermentans | A9KHK4 | bacterium possesses three GalHexNAcP homologous genes, cphy1920 gene; anaerobic cellulolytic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lachnoclostridium phytofermentans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose + alpha-D-galactose 1-phosphate | when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-2-deoxy-D-glucose + phosphate | - |
r | |
| alpha-D-galactose 1-phosphate + L-rhamnose | highest activity | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,4-L-rhamnose + phosphate | - |
r | |
| D-galactose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-D-galactose + phosphate | - |
r | |
| D-glucose + alpha-D-galactose 1-phosphate | when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-D-glucose + phosphate | - |
r | |
| L-lyxose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | beta-D-galactosyl-(1->4)-L-lyxose + phosphate | - |
r | |
| L-mannose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | D-galactosyl-beta-1,4-L-mannose + phosphate | - |
r | |
| additional information | phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP | Lachnoclostridium phytofermentans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cphy1920 protein | - |
Lachnoclostridium phytofermentans |
| D-galactosyl-beta-1,4-L-rhamnose phosphorylase | - |
Lachnoclostridium phytofermentans |
| GalRhaP | - |
Lachnoclostridium phytofermentans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
protein is thermostable up to 37°C | Lachnoclostridium phytofermentans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 8 | 2-deoxy-D-glucose | - |
Lachnoclostridium phytofermentans | |
| 24 | - |
D-galactose | - |
Lachnoclostridium phytofermentans | |
| 45 | - |
D-galactosyl-beta-1,4-L-rhamnose | - |
Lachnoclostridium phytofermentans | |
| 74 | - |
L-rhamnose | - |
Lachnoclostridium phytofermentans | |
| 85 | - |
D-glucose | - |
Lachnoclostridium phytofermentans | |
| 120 | - |
L-Lyxose | - |
Lachnoclostridium phytofermentans | |
| 170 | - |
L-Mannose | - |
Lachnoclostridium phytofermentans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | optimum activity at neutral pH | Lachnoclostridium phytofermentans |
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