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Information on EC 2.4.1.237 - flavonol 7-O-beta-glucosyltransferase
for references in articles please use BRENDA:EC2.4.1.237
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.237 flavonol 7-O-beta-glucosyltransferase
IUBMB Comments
Acts on the flavonols gossypetin (8-hydroxyquercetin) and to a lesser extent on quercetin, kaempferol and myricetin.
The enzyme appears in viruses and cellular organisms
- 2.4.1.237
- thiolactomycin
- flavonols
-
glucosylation
-
beta-ketoacyl-acp
-
mycolic
- petals
-
condensing
- flavanones
- coumarins
-
7-hydroxyl
showSynonyms
ntgt2, ugt73a4, ugt71f1, udp-glucose:flavonoid 7-o-glucosyltransferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
flavonol 7-O-glucosyltransferase
Q7OGT
quercetin 7OGT
quercetin-7-O-GT
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a flavonol = UDP + a flavonol 7-O-beta-D-glucoside
acts on the flavonols gossypetin (8-hydroxyquercetin) and to a lesser extend on quercetin, kaempferol and myricetin
-
UDP-glucose + a flavonol = UDP + a flavonol 7-O-beta-D-glucoside
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PATHWAY SOURCE
PATHWAYS
MetaCyc
eupatolitin 3-O-glucoside biosynthesis, gossypetin metabolism, kaempferol glycoside biosynthesis (Arabidopsis), quercetin glucoside biosynthesis (Allium), quercetin glycoside biosynthesis (Arabidopsis)
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:flavonol 7-O-beta-D-glucosyltransferase
Acts on the flavonols gossypetin (8-hydroxyquercetin) and to a lesser extent on quercetin, kaempferol and myricetin.
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CAS REGISTRY NUMBER
COMMENTARY
83682-90-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
-
Substrates: 17% of the activity with quercetin, UGT71F1
Products: -
Products: -
?
UDP-alpha-D-glucose + luteolin
UDP + luteolin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + naringenin
UDP + naringenin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside + quercetin 3-O-beta-D-glucoside
-
Substrates: UGT71F1
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside + quercetin 4'-O-beta-D-glucoside
-
Substrates: 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4
Products: -
Products: -
?
UDP-glucose + 1-naphthol
UDP + naphthol 1-O-beta-D-glucoside
-
Substrates: 44% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + 2-coumaric acid
UDP + ?
-
Substrates: 41% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + 2-naphthol
UDP + naphthol 2-O-beta-D-glucoside
-
Substrates: 14% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + 3,5,7-trihydroxyflavanone
UDP + 3,5,7-trihydroxyflavanone 7-O-beta-D-glucoside
-
Substrates: pinobanksin, 55% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + 3-hydroxycoumarin
UDP + coumarin 3-O-beta-D-glucoside
-
Substrates: 310% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + 3-hydroxyflavone
UDP + 3-hydroxyflavone 7-O-beta-D-glucoside
-
Substrates: 12% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + 4-coumaric acid
UDP + ?
-
Substrates: 43% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + 6-hydroxyflavone
UDP + 6-hydroxyflavone 7-O-beta-D-glucoside
-
Substrates: 24% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + 7,4'-dihydroxyflavonol
UDP + 7,4'-dihydroxyflavonol 7-O-beta-D-glucoside
-
Substrates: 53% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + 7-hydroxyflavone
UDP + 7-hydroxyflavone 7-O-beta-D-glucoside
-
Substrates: 53% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + betanidin
UDP + betanidin 5-O-beta-D-glucoside + betanidin 6-O-beta-D-glucoside
-
Substrates: 28% of the activity with quercetin, UGT71F1
Products: -
Products: -
?
UDP-glucose + caffeic acid
UDP + caffeic acid 4-O-beta-D-glucoside
-
Substrates: 14% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + caffeic acid
UDP + caffeoyl 3-O-beta-D-glucoside
-
Substrates: 90% of the activity with quercetin, UGT73A4
Products: -
Products: -
?
UDP-glucose + chrysin
UDP + chrysin 7-O-beta-D-glucoside
-
Substrates: 70% of the activity with quercetinUGT73A4
Products: -
Products: -
?
UDP-glucose + esculetin
UDP + esculetin 6-O-beta-D-glucoside
-
Substrates: 77% of the activity with quercetin, UGT73A4
Products: -
Products: -
?
UDP-glucose + esculetin
UDP + esculetin 7-O-beta-D-glucoside
-
Substrates: 24% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + euonymin
UDP + euonymin 7-O-beta-D-glucoside
-
Substrates: 16% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + hydroquinone
UDP + hydroquinone O-beta-D-glucoside
-
Substrates: 10% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + isoquercitrin
UDP + isoquercitrin 7-O-beta-D-glucoside
-
Substrates: 13% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferide
UDP + kaempferide 7-O-beta-D-glucoside
-
Substrates: 104% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol 3,4'-di-O-glucoside
UDP + kaempferol 3,7,4'-tri-O-glucoside
-
Substrates: 12% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol 3,4'-dimethylether
UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside
UDP-glucose + kaempferol 3-O-glucoside
UDP + kaempferol 3,7-O-beta-D-diglucoside
-
Substrates: astragalin, 17% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol 3-O-glucoside
UDP + kaempferol 3,7-O-diglucoside
-
Substrates: astragalin, 20% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol 3-O-glucoside
UDP + kaempferol 3,7beta-O-diglucoside
-
Substrates: 45% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + kaempferol 3-O-rhamnosylglucoside
UDP + kaempferol 3-O-rhamnosylglucoside 7-O-beta-D-glucoside
-
Substrates: 11% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol 5,7,4'-trimethylether
UDP + kaempferol 5,7,4'-trimethylether 3-O-beta-D-glucoside
-
Substrates: 9% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol-4'-methylether
UDP + kaempferol-4'-methylether 7-O-beta-D-glucoside
-
Substrates: 42% of the activity with quercetin, UGT73A4
Products: -
Products: -
?
UDP-glucose + myricetin
UDP + myricetin 4'-O-beta-D-glucoside
-
Substrates: 80% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4
Products: -
Products: -
?
UDP-glucose + p-coumaric acid
UDP + (2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
-
Substrates: 9% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + quercetagetin
UDP + quercetagetin 3-O-beta-D-glucoside + quercetagetin 7-O-beta-D-glucoside
-
Substrates: 84% of the activity with quercetin, UGT71F1
Products: -
Products: -
?
UDP-glucose + quercetagetin
UDP + quercetagetin 4'-O-beta-D-glucoside
-
Substrates: 28% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4
Products: -
Products: -
?
UDP-glucose + quercetin 3-O-glucoside
UDP + quercetin 3,7-O-beta-D-diglucoside
-
Substrates: isoquercetin, 32% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + quercetin 5-methylether
UDP + quercetin 5-methylether 7-O-beta-D-glucoside
-
Substrates: 13% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + rutin
UDP + rutin 7-O-beta-D-glucoside
-
Substrates: 21% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: 81% of the activity with quercetin, UGT73A4
Products: -
Products: -
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: good substrate
Products: -
Products: -
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin
Products: -
Products: -
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: preferred substrate
Products: -
Products: -
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: best substrate
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: 29% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: gossypetin and quercetin are presumably the natural substrates
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: cv. Prado: gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times lower specific activity than with gossypetin as substrate
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: 19% of the activity with kaempferol
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: 45% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
Substrates: -
Products: F7GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some other flavonols, highly specific for UDP-D-glucose as glucosyl donor
Products: F7GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some other flavonols, highly specific for UDP-D-glucose as glucosyl donor
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
Substrates: plays a role in the glycosylation steps of flavonols
Products: -
Products: -
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
Substrates: F 7 GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some flavonols, highly specific for UDP-D-glucose
Products: -
Products: -
?
UDP-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
-
Substrates: 90% of the activity with quercetin, UGT73A4
Products: -
Products: -
?
UDP-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
-
Substrates: 40% of the activity with quercetin, UGT71F1
Products: -
Products: -
?
UDP-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
-
Substrates: cv. Prado
Products: -
Products: -
?
UDP-glucose + eriodictyol
UDP + eriodictyol 7-O-beta-D-glucoside
-
Substrates: 48% of the activity with quercetin, UGT73A4
Products: -
Products: -
?
UDP-glucose + eriodictyol
UDP + eriodictyol 7-O-beta-D-glucoside
-
Substrates: cv. Prado
Products: -
Products: -
?
UDP-glucose + gossypetin
UDP + gossypetin 7-O-beta-D-glucoside
-
Substrates: gossypetin and quercetin are presumably the natural substrates
Products: gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals
Products: gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals
?
UDP-glucose + gossypetin
UDP + gossypetin 7-O-beta-D-glucoside
-
Substrates: cv. Prado: best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times higher specific activity than with quercetin as substrate
Products: -
Products: -
?
UDP-glucose + isorhamnetin
UDP + isorhamnetin 7-O-beta-D-glucoside
-
Substrates: 128% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + isorhamnetin
UDP + isorhamnetin 7-O-beta-D-glucoside
-
Substrates: 88% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + kaempferol 3,4'-dimethylether
UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside
-
Substrates: 31% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + kaempferol 3,4'-dimethylether
UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside
-
Substrates: 30% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + luteolin
UDP + luteolin 7-O-beta-D-glucoside
-
Substrates: as active as with quercetin, UGT73A4
Products: -
Products: -
?
UDP-glucose + luteolin
UDP + luteolin 7-O-beta-D-glucoside
-
Substrates: 17% of the activity with quercetin, UGT71F1
Products: -
Products: -
?
UDP-glucose + luteolin
UDP + luteolin 7-O-beta-D-glucoside
-
Substrates: cv. Prado
Products: -
Products: -
?
UDP-glucose + myricetin
UDP + myricetin 7-O-beta-D-glucoside
-
Substrates: 36% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + myricetin
UDP + myricetin 7-O-beta-D-glucoside
-
Substrates: cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin
Products: -
Products: -
?
UDP-glucose + myricetin
UDP + myricetin 7-O-beta-D-glucoside
-
Substrates: 26% of the activity with kaempferol as substrate
Products: -
Products: -
?
UDP-glucose + naringenin
UDP + naringenin 7-O-beta-D-glucoside
-
Substrates: 10% of the activity with quercetin, UGT73A4
Products: -
Products: -
?
UDP-glucose + naringenin
UDP + naringenin 7-O-beta-D-glucoside
-
Substrates: cv. Prado
Products: -
Products: -
?
UDP-glucose + pinocembrin
UDP + pinocembrin 7-O-beta-D-glucoside
-
Substrates: 25% of the activity with quercetinUGT73A4
Products: -
Products: -
?
UDP-glucose + pinocembrin
UDP + pinocembrin 7-O-beta-D-glucoside
-
Substrates: 57% of the activity with quercetin, UGT71F1
Products: -
Products: -
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + scopoletin
UDP + scopoletin 7-O-beta-D-glucoside
-
Substrates: 11% of the activity with kaempferol
Products: -
Products: -
?
UDP-glucose + scopoletin
UDP + scopoletin 7-O-beta-D-glucoside
-
Substrates: 8% of the activity with kaempferol as substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: glycosylation of flavonols
Products: -
Products: -
?
additional information
?
-
-
Substrates: no or very poor substrates: fisetin, kaempferol 5,7,4-trimethylether, rhamnetin, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, taxifolin, dihydrokaempferol, ADP-D-glucose, GDP-D-glucose
Products: -
Products: -
?
additional information
?
-
-
Substrates: flavonol glycoside pathway
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrates: 3-/7-O-glucosylated flavonols and UDP-rhamnose
Products: -
Products: -
-
additional information
?
-
-
Substrates: broad substrate specificity, no activity with salicylic acid, cyanidin, and cyanidin 3-O-glucoside, poor activity with 4-hydroxycoumarin and 5-hydroxyflavone
Products: -
Products: -
?
additional information
?
-
-
Substrates: not: rhamnetin, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, ADP-D-glucose, GDP-D-glucose
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity of isozymes with diverse different flavonol compounds, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity of isozymes with diverse different flavonol compounds, overview
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: gossypetin and quercetin are presumably the natural substrates
Products: -
Products: -
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
Substrates: plays a role in the glycosylation steps of flavonols
Products: -
Products: -
?
UDP-glucose + gossypetin
UDP + gossypetin 7-O-beta-D-glucoside
-
Substrates: gossypetin and quercetin are presumably the natural substrates
Products: gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals
Products: gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: glycosylation of flavonols
Products: -
Products: -
?
additional information
?
-
-
Substrates: flavonol glycoside pathway
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4,4'-diisothiocyanostilbene-2,2'-disulfonic acid
-
0.01 mM, UGT71F1; 0.01 mM, UGT73A4
diethyldicarbonate
-
5 mM, 98% inhibition with quercetin or gossypetin as substrate
p-chloromercuribenzene sulfonic acid
-
UGT71F1, 0.01mM, 22% inhibition; UGT73A4 activity is completely inhibited at 0.01mM
p-hydroxymercuribenzoate
-
strong, 0.1 mM, 99.3% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects and addition after 15 min also reduces the inhibitory effect
Co2+
-
complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4
Co2+
-
1 mM, 98% inhibition with quercetin as substrate, 85% inhibition with gossypetin as substrate
Cu2+
-
complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4
N-ethylmaleimide
-
1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity
N-ethylmaleimide
-
strong, 1 mM, 98% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects, but addition after 15 min does not restore activity
N-ethylmaleimide
-
1 mM, 94% inhibition, 14 mM 2-mercaptoethanol restores activity
p-chloromercuribenzoate
-
1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity
p-chloromercuribenzoate
-
1 mM, 91% inhibition, 14 mM 2-mercaptoethanol restores activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
bovine serum albumin
-
addition to the assay system causes 2.5fold higher reaction rate
-
additional information
-
enzyme expression is inducible by plant hormones, such as salicylic acid, auxin, 2,4-dichorophenoxyacetic acid, and jasmonate
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
specific activities for the 7-O-glucosylation reaction with several substrates, cv. Stern des Orients: 3times higher specific activity with gossypetin than with quercetin as substrate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
additional information
-
the pH-optimum is highly dependent on the substrate used
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5
5.6
-
calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT71F1
5.8
-
calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT73A4
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
-
Concord flower bud, leaf and flower tissues all have variable levels of Q7GT
brenda
-
chiefly localized in the parenchyma, tissue-specific distribution in leaves
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40
-
10 min, no influence on the reaction up to 40°C, higher temperatures lead to loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 15% loss of activity, 8 days: 60% loss of activity
-
-20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 18% loss of activity, 8 days: 64% loss of activity
-
UGT71F1 can not be frozen nor stored at 4°C as 50% activity is lost within 24 h
-
UGT73A4 can be stored at 4°C or frozen at -20°C or -80°C for several months without requiring addition of stabilizing agents such as bovine serum albumin or glycerol
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT71F1
-
functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT73A4
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
-
Escherichia coli expressing tobacco-derived glucosyltransferase yields 24 mg/l of apigenin 7-O-glucoside, 41 mg/l of luteolin 7-O-glucoside, 118 mg/l of quercetin 7-O-glucoside, 40 mg/l of kaempferol 7-O-glucoside, and 75 mg/l of naringenin 7-O-glucoside through sequential administration of substrates in 4-9 h in scaled-up production
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ishikura, N.; Yang, Z.Q.
Multiple forms of flavonol O-glucosyltransferases in young leaves of Euonymus alatus f. ciliato-denatus
Phytochemistry
36
1139-1145
1994
Euonymus alatus
-
brenda
Ishikura, N.; Yang, Z.Q.; Teramoto, S.
UDP-D-glucose:flavonol 3-O- and 7-O-glucosyl transferases from young leaves of Paederia seandens var. mairei
Z. Naturforsch. C
48
563-569
1993
Paederia scandens
-
brenda
Hrazdina, G.; Marx, G.A.; Hoch, H.C.
Distribution of secondary plant metabolites and their biosynthetic enzymes in pea (Pisum sativum L.) leaves. Anthocyanins and flavonol glycosides
Plant Physiol.
70
745-748
1982
Lathyrus oleraceus
brenda
Stich, K.; Halbwirth, H.; Wurst, F.; Forkmann, G.
UDP-glucose:flavonol 7-O-glucosyltransferase activity in flower extracts of Chrysanthemum segetum
Z. Naturforsch. C
52
153-158
1997
Glebionis segetum
brenda
Taguchi, G.; Ubukata, T.; Hayashida, N.; Yamamoto, H.; Okazaki, M.
Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxylgroup of coumarin from tobacco cells
Arch. Biochem. Biophys.
420
95-102
2003
Nicotiana tabacum
brenda
Isayenkova, J.; Wray, V.; Nimtz, M.; Strack, D.; Vogt, T.
Cloning and functional characterization of two regioselective flavonoid glucosyltransferases from Beta vulgaris
Phytochemistry
67
1598-1612
2006
Beta vulgaris
brenda
Hall, D.; Kim, K.; De Luca, V.
Molecular cloning and biochemical characterization of three Concord grape (Vitis labrusca) flavonol 7-O-glucosyltransferases
Planta
234
1201-1214
2011
Vitis labrusca, Vitis labrusca Concord
brenda
Dorjjugder, N.; Taguchi, G.
Production of flavonoid 7-O-glucosides by bioconversion using Escherichia coli expressing a 7-O-glucosyltransferase from tobacco (Nicotiana tabacum)
Appl. Biochem. Biotechnol.
194
3320-3329
2022
Nicotiana tabacum
brenda
Chen, G.; Li, D.; Yao, P.; Chen, F.; Yuan, J.; Ma, B.; Yang, Z.; Ding, B.; He, N.
Metabolic and transcriptional analysis reveals flavonoid involvement in the drought stress response of mulberry leaves
Int. J. Mol. Sci.
25
7417
2024
Morus alba
brenda
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