Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | addition to the assay system causes 2.5fold higher reaction rate | Glebionis segetum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 98% inhibition with quercetin as substrate, 85% inhibition with gossypetin as substrate | Glebionis segetum | |
Cu2+ | 1 mM, 99% inhibition with quercetin as substrate | Glebionis segetum | |
diethyldicarbonate | 5 mM, 98% inhibition with quercetin or gossypetin as substrate | Glebionis segetum | |
Fe2+ | 1 mM, 98% inhibition with quercetin as substrate | Glebionis segetum | |
Hg2+ | 1 mM, 99% inhibition with quercetin as substrate | Glebionis segetum | |
additional information | not inhibited by EDTA | Glebionis segetum | |
N-ethylmaleimide | strong, 1 mM, 98% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects, but addition after 15 min does not restore activity | Glebionis segetum | |
p-hydroxymercuribenzoate | strong, 0.1 mM, 99.3% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects and addition after 15 min also reduces the inhibitory effect | Glebionis segetum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
kaempferol | pH 7.8, 25°C | Glebionis segetum | |
0.032 | - |
gossypetin | pH 6.9, 25°C | Glebionis segetum | |
0.049 | - |
quercetin | pH 8.3, 25°C | Glebionis segetum | |
0.067 | - |
myricetin | pH 8.1, 25°C | Glebionis segetum | |
0.071 | - |
eriodictyol | pH 7.5, 25°C | Glebionis segetum | |
0.073 | - |
naringenin | pH 6.9, 25°C | Glebionis segetum | |
0.078 | - |
apigenin | pH 7.7, 25°C | Glebionis segetum | |
0.084 | - |
luteolin | pH 8.1, 25°C | Glebionis segetum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight stimulation | Glebionis segetum | |
KCN | slight stimulation | Glebionis segetum | |
Mg2+ | slight stimulation | Glebionis segetum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + quercetin | Glebionis segetum | gossypetin and quercetin are presumably the natural substrates | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + gossypetin | Glebionis segetum | gossypetin and quercetin are presumably the natural substrates | UDP + gossypetin 7-O-beta-D-glucoside | gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glebionis segetum | - |
cv. Prado and Stern des Orients | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + a flavonol = UDP + a flavonol 7-O-beta-D-glucoside | acts on the flavonols gossypetin (8-hydroxyquercetin) and to a lesser extend on quercetin, kaempferol and myricetin | Glebionis segetum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
petal | - |
Glebionis segetum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities for the 7-O-glucosylation reaction with several substrates, cv. Stern des Orients: 3times higher specific activity with gossypetin than with quercetin as substrate | Glebionis segetum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + kaempferol | cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin | Glebionis segetum | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | gossypetin and quercetin are presumably the natural substrates | Glebionis segetum | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | cv. Prado: gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times lower specific activity than with gossypetin as substrate | Glebionis segetum | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavonol | - |
Glebionis segetum | UDP + a flavonol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + apigenin | cv. Prado | Glebionis segetum | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + eriodictyol | cv. Prado | Glebionis segetum | UDP + eriodictyol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + gossypetin | gossypetin and quercetin are presumably the natural substrates | Glebionis segetum | UDP + gossypetin 7-O-beta-D-glucoside | gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals | ? | |
UDP-glucose + gossypetin | cv. Prado: best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times higher specific activity than with quercetin as substrate | Glebionis segetum | UDP + gossypetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + luteolin | cv. Prado | Glebionis segetum | UDP + luteolin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + myricetin | cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin | Glebionis segetum | UDP + myricetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + naringenin | cv. Prado | Glebionis segetum | UDP + naringenin 7-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose:flavonol 7-O-glucosyltransferase | - |
Glebionis segetum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Glebionis segetum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
7-O-glucosylation also proceeds at low temperatures, e.g. 0°C | Glebionis segetum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, no influence on the reaction up to 40°C, higher temperatures lead to loss of activity | Glebionis segetum |
60 | - |
10 min, incubation without UDP-glucose, complete loss of activity | Glebionis segetum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the pH-optimum is highly dependent on the substrate used | Glebionis segetum |
6.9 | - |
gossypetin or naringenin as substrate | Glebionis segetum |
7.5 | - |
eriodictyol as substrate | Glebionis segetum |
7.7 | - |
apigenin as substrate | Glebionis segetum |
7.8 | - |
kaempferol as substrate | Glebionis segetum |
8.1 | - |
myricetin or luteolin as substrate | Glebionis segetum |
8.3 | - |
quercetin as substrate | Glebionis segetum |