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Information on EC 2.3.1.273 - diglucosylglycerate octanoyltransferase
for references in articles please use BRENDA:EC2.3.1.273
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EC Tree 2 Transferases
2.3 Acyltransferases
2.3.1 Transferring groups other than aminoacyl groups
2.3.1.273 diglucosylglycerate octanoyltransferase
IUBMB Comments
The enzyme, characterized from mycobacteria, is involved in the biosynthesis of methylglucose lipopolysaccharide (MGLP). The enzyme can also act on 2-O-(α-D-glucopyranosyl)-D-glycerate, but with lower activity.
The expected taxonomic range for this enzyme is: Mycobacteriaceae
Reaction Schemes
showSynonyms
DGG octanoyltransferase, octT, Rv2418c, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DGG octanoyltransferase
octT
DGG octanoyltransferase
-
-
-
-
octT
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate = CoA + 2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
octanoyl-CoA:2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate octanoyltransferase
The enzyme, characterized from mycobacteria, is involved in the biosynthesis of methylglucose lipopolysaccharide (MGLP). The enzyme can also act on 2-O-(alpha-D-glucopyranosyl)-D-glycerate, but with lower activity.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
hexanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-hexanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
hexanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-hexanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: -
Products: -
Products: -
?
hexanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-hexanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: -
Products: -
Products: -
?
octanoyl-CoA + 2-O-(alpha-D-glucopyranosyl)-D-glycerate
?
Substrates: lower activity than with 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
Products: -
Products: -
?
octanoyl-CoA + 2-O-(alpha-D-glucopyranosyl)-D-glycerate
?
Substrates: lower activity than with 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: -
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: -
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide
Products: -
Products: -
?
octanoyl-CoA + 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate + CoA
Substrates: the enzyme is involved in the biosynthesis of methylglucose lipopolysaccharide
Products: -
Products: -
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
0.4 mM, about 20% inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6.6
2-O-(alpha-D-glucopyranosyl)-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
19
2-O-(alpha-D-glucopyranosyl)-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
9.5
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
9.8
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA
20
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.01
hexanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.02
hexanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
0.01
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.02
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.03
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
0.06
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00033
2-O-(alpha-D-glucopyranosyl)-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.00033
2-O-(alpha-D-glucopyranosyl)-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.0005
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA
0.0017
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.038
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.00017
hexanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.00067
hexanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
0.00017
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.00033
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.002
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
0.037
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00005
2-O-(alpha-D-glucopyranosyl)-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.002
2-O-(alpha-D-glucopyranosyl)-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.00005
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM hexanoyl-CoA
0.00017
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.0018
2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
37°C, pH 8.0, cosubstrate: 0.1 mM octanoyl-CoA
0.022
hexanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.035
hexanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
0.018
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.025
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 45 mM 2-O-(alpha-D-glucopyranosyl)-D-glycerate
0.032
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 20 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
1.43
octanoyl-CoA
37°C, pH 8.0, cosubstrate: 45 mM 2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-D-glycerate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 50
30°C: about 40% of maximal activity, 50°C: about 50% of maximal activity
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
metabolism
the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide
metabolism
the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide
metabolism
the enzyme is essential for gowth of Mycobacterium tuberculosis. It is involved in the biosynthesis of methylglucose lipopolysaccharide
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). OCTT_MYCS2
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
252
0
27762
Swiss-Prot
other Location (Reliability: 2)
OCTT_MYCHD
Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)
240
0
26579
Swiss-Prot
-
OCTT_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
247
0
27225
Swiss-Prot
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a His-tagged enzyme
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Maranha, A.; Moynihan, P.J.; Miranda, V.; Correia Lourenco, E.; Nunes-Costa, D.; Fraga, J.S.; Jose Barbosa Pereira, P.; Macedo-Ribeiro, S.; Ventura, M.R.; Clarke, A.J.; Empadinhas, N.
Octanoylation of early intermediates of mycobacterial methylglucose lipopolysaccharides
Sci. Rep.
5
13610
2015
Mycobacterium tuberculosis (P71725), Mycolicibacterium hassiacum (K5BJH8), Mycolicibacterium smegmatis (A0R109)
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