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IUBMB CommentsPyrrolidine and 3-pyrroline can also act as acceptors, but more slowly.
The expected taxonomic range for this enzyme is: Piper nigrum
Synonyms
piperidine piperoyltransferase, piperoyl-CoA:piperidine N-piperoyltransferase, more
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piperidine piperoyltransferase
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piperoyl-CoA:piperidine N-piperoyltransferase
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(E,E)-piperoyl-CoA + piperidine = CoA + N-[(E,E)-piperoyl]-piperidine
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Acyl group transfer
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MetaCyc
piperine biosynthesis
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(E,E)-piperoyl-CoA:piperidine N-piperoyltransferase
Pyrrolidine and 3-pyrroline can also act as acceptors, but more slowly.
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(E,E)-piperoyl-CoA + piperidine
CoA + N-[(E,E)-piperoyl]-piperidine
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piperoyl-CoA + 3-pyrroline
CoA + piperoyl-(3-pyrroline)
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Substrates: 25% of activity with piperidine
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piperoyl-CoA + pyrrolidine
CoA + piperoylpyrrolidine
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Substrates: 76% of activity with piperidine
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(E,E)-piperoyl-CoA + piperidine
CoA + N-[(E,E)-piperoyl]-piperidine
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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PS_PIPNI
461
0
51169
Swiss-Prot
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40 - 42
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heat-denaturation
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10 mM 2-mercaptoethanol stabilizes
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preparations are to be carried out at 0-4°C
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cell homogenate has to be supplemented with 50 mM ascorbic acid as antioxidant
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486332
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0-4°C, enzyme stored as ammonium sulfate paste loses 20-25% of activity within one week, completely inactive after two weeks
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diluted enzyme stored after anion-exchange chromatography is unstable
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Geisler, J.G.; Gross, G.G.
The biosynthesis of piperine in Piper nigrum
Phytochemistry
29
489-492
1990
Piper nigrum
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brenda
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