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Information on EC 2.1.1.150 - isoflavone 7-O-methyltransferase
for references in articles please use BRENDA:EC2.1.1.150
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EC Tree 2 Transferases
2.1 Transferring one-carbon groups
2.1.1 Methyltransferases
2.1.1.150 isoflavone 7-O-methyltransferase
IUBMB Comments
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4′-trihydroxyisoflavone but not flavones or flavanones.
The enzyme appears in viruses and cellular organisms
- 2.1.1.150
-
isoflavonoids
- alfalfa
-
phytoalexins
- daidzein
-
medicago
- sativa
- medicarpin
- formononetin
-
pisum
-
phenylpropanoid
-
regiospecific
-
leguminous
- chalcone
-
4'-o-methylated
-
b-rings
-
7-hydroxyl
-
caffeic
-
4'-o-methyltransferase
-
7-o-methylation
-
cinnamic
-
4\'-hydroxyl
-
elicitor-induced
- chickpea
-
pterocarpan
-
elicitor-treated
Reaction Schemes
showSynonyms
hi4'omt, isoflavone o-methyltransferase, iomt8, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
HI4'OMT
-
-
-
-
IOMT
-
-
-
-
IOMT8
isoflavone methyltransferase
-
-
-
-
isoflavone O-methyltransferase
methyltransferase, 4'-hydroxyisoflavone
-
-
-
-
isoflavone O-methyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
isoflavonoid biosynthesis I, isoflavonoid biosynthesis II
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
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CAS REGISTRY NUMBER
COMMENTARY
136111-54-1
-
55071-80-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3'-hydroxydaidzein
S-adenosyl-L-homocysteine + 7,4'-dihydroxy-3'-methoxyisoflavone
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
S-adenosyl-L-methionine + 6-hydroxydaidzein
S-adenosyl-L-homocysteine + 7,4'-dihydroxy-6-methoxyisoflavone
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,2'-dihydroxy-4'-methoxyisoflavone
S-adenosyl-L-homocysteine + 2-hydroxy-7,4'-dimethoxyisoflavone
-
Substrates: i.e. 2'-hydroxyformononetin. 55% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,3',4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 3',4'-dihydroxy-7-methoxyisoflavone
S-adenosyl-L-methionine + 7,4'-dihydroxy-6-methoxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-6,7-dimethoxyisoflavone
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavanone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavanone
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
S-adenosyl-L-methionine + 8-hydroxydaidzein
S-adenosyl-L-homocysteine + 7,4'-dihydroxy-8-methoxyisoflavone
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
Substrates: i.e. genistein, about 7% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
Substrates: i.e. genistein. 50% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
Substrates: i.e. genistein. 12.9% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
Substrates: i.e. genistein. 49% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
Substrates: i.e. genistein. 61% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
Substrates: (i.e. glycitein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
Substrates: i.e. glycitein. 178% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
Substrates: 136% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
Substrates: 44% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
Substrates: 35% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
Substrates: preferred substrate
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,3',4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 3',4'-dihydroxy-7-methoxyisoflavone
Substrates: 41% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,3',4'-trihydroxyisoflavone
S-adenosyl-L-homocysteine + 3',4'-dihydroxy-7-methoxyisoflavone
Substrates: 43% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,4'-dihydroxy-6-methoxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-6,7-dimethoxyisoflavone
Substrates: i.e. glycitein, preferred substrate
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,4'-dihydroxy-6-methoxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-6,7-dimethoxyisoflavone
Substrates: i.e. glycitein, 39% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,4'-dihydroxy-6-methoxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-6,7-dimethoxyisoflavone
Substrates: i.e. glycitein, 52% the activity with 6,7,4'-trihydroxyisoflavone (i.e. daidzein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavanone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavanone
Substrates: i.e. dihydrodaidzein. 15.6% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavanone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavanone
Substrates: i.e. dihydrodaidzein. 70% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein)
Products: -
Products: -
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein). Key enzyme in the biosynthesis of the phytoalexin medicarpin in Medicago sativa
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
Substrates: i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein
Products: i.e. isoformononetin
Products: i.e. isoformononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulumassociated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein)
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
Substrates: i.e. daidzein
Products: i.e. isoformononetin
Products: i.e. isoformononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
Substrates: i.e. daidzein
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. The activity of alfalfa IOMT as an elicitor-inducible, operationally soluble enzyme with 7-position specificity for daidzein in vitro
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
Substrates: i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
Substrates: i.e. daidzein
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulumassociated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. The activity of alfalfa IOMT as an elicitor-inducible, operationally soluble enzyme with 7-position specificity for daidzein in vitro
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. 21% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein)
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. Preferred substrate
Products: -
Products: -
?
additional information
?
-
Substrates: IOMT1 displays almost the same activity with 6-hydroxydaidzein and 8-hydroxydaidzein. The activity with 3'-hydroxydaidzein is 2-fold weaker
Products: -
Products: -
-
additional information
?
-
Substrates: no activity with apigenin (a flavone), naringenine (a falvanone)
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with apigenin (a flavone), naringenine (a falvanone)
Products: -
Products: -
?
additional information
?
-
-
Substrates: IOMT II does not catalyse the methylation of apigenin, kaempferol, flavonoids mono-hydroxylated in the 2,3,4,5, or 7 positions, flavanones such as naringenin or coumestans such as coumestrol
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 6,7-dihydroxy-4'-methoxyisoflavone
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with 6,7-dihydroxy-4'-methoxyisoflavone
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with 6,7-dihydroxy-4'-methoxyisoflavone
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein). Key enzyme in the biosynthesis of the phytoalexin medicarpin in Medicago sativa
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
Substrates: i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein
Products: i.e. isoformononetin
Products: i.e. isoformononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulumassociated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
Substrates: i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
Products: i.e. formononetin
Products: i.e. formononetin
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
Substrates: i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulumassociated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion
Products: i.e. formononetin
Products: i.e. formononetin
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
enzyme activity was not affected by inclusion of 5 mM Mn2+, Mg2+, or EDTA in the assay mixture
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
IOMT colocalizes with the endoplasmic reticulumassociated isoflavone synthase cytochrome P450
brenda
-
IOMT colocalizes with the endoplasmic reticulumassociated isoflavone synthase cytochrome P450
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
IOMT1 overexpression increases the levels of glycitein derivatives in soybean hairy roots when flavonoid 6-hydroxylase is coexpressed
Show AA Sequence and Transmembrane Helices (399 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
41000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method. Crystal structure of IOMT in complex with the products S-adenosyl-L-homocysteine and isoformononetin (4'-hydroxy-7-methoxyisoflavone) refined to 1.4 A
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
up
IOMT transcripts are rapidly induced following elicitation, prior to the increase in IOMT activity
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
treatment of alfalfa cell suspension cultures with elicitor preparations from Saccharomyces cerevisiae or from cell walls of Colletotrichum lindemuthianum results in a ca 200fold induction of isoflavone 0-methyltransferase activity
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dalkin, K.; Edwards, R.; Edington, B.; Dixon, R.A.
Stress responses in alfalfa (Medicago sativa L.). I. Induction of phenylpropanoid biosynthesis and hydrolytic enzymes in elicitor-treated cell suspension cultures
Plant Physiol.
92
440-446
1990
Medicago sativa
brenda
He, X.Z.; Dixon, R.A.
Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa
Plant Cell
12
1689-1702
2000
Medicago sativa, Medicago sativa cv Regen SY
brenda
He, X.Z.; Dixon, R.A.
Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.)
Arch. Biochem. Biophys.
336
121-129
1996
Medicago sativa (O24529), Medicago sativa
brenda
He, X.Z.; Reddy, J.T.; Dixon, R.A.
Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase
Plant Mol. Biol.
36
43-54
1998
Medicago sativa (O24529), Medicago sativa
brenda
Liu, C.J.; Dixon, R.A.
Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis
Plant Cell
13
2643-2658
2001
Medicago sativa (O24529), Medicago sativa, Medicago sativa cv Regen SY (O24529)
brenda
Zubieta, C.; He, X.Z.; Dixon, R.A.; Noel, J.P.
Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases
Nat. Struct. Biol.
8
271-279
2001
Medicago sativa (O24529), Medicago sativa
brenda
Deavours, B.E.; Liu, C.J.; Naoumkina, M.A.; Tang, Y.; Farag, M.A.; Sumner, L.W.; Noel, J.P.; Dixon, R.A.
Functional analysis of members of the isoflavone and isoflavanone O-methyltransferase enzyme families from the model legume Medicago truncatula
Plant Mol. Biol.
62
715-733
2006
Medicago truncatula, Medicago truncatula (Q06YR4), Medicago truncatula (Q06YR3)
brenda
Uchida, K.; Sawada, Y.; Ochiai, K.; Sato, M.; Inaba, J.; Hirai, M.Y.
Identification of a unique type of isoflavone O-methyltransferase, GmIOMT1, based on multi-omics analysis of soybean under biotic stress
Plant Cell Physiol.
61
1974-1985
2020
Glycine max (C6T2E2)
brenda
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