Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | 10-20 mM, up to 39% inhibition | Medicago sativa | |
dithiothreitol | 10-20 mM, up to 39% inhibition | Medicago sativa |
General Stability | Organism |
---|---|
stabilized in the presence of EDTA and thiol-containing reagents | Medicago sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity was not affected by inclusion of 5 mM Mn2+, Mg2+, or EDTA in the assay mixture | Medicago sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, SDS-PAGE | Medicago sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Medicago sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Medicago sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | IOMT II does not catalyse the methylation of apigenin, kaempferol, flavonoids mono-hydroxylated in the 2,3,4,5, or 7 positions, flavanones such as naringenin or coumestans such as coumestrol | Medicago sativa | ? | - |
? | |
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone | i.e. genistein. 50% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) | Medicago sativa | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone | - |
? | |
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone | i.e. glycitein. 178% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) | Medicago sativa | S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone | - |
? | |
S-adenosyl-L-methionine + 7,2'-dihydroxy-4'-methoxyisoflavone | i.e. 2'-hydroxyformononetin. 55% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) | Medicago sativa | S-adenosyl-L-homocysteine + 2-hydroxy-7,4'-dimethoxyisoflavone | - |
? | |
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | i.e. daidzein | Medicago sativa | S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone | i.e. isoformononetin | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Medicago sativa |
Synonyms | Comment | Organism |
---|---|---|
IOMT II | - |
Medicago sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
Tris buffer | Medicago sativa |
8.7 | - |
glycine-NaOH buffer | Medicago sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Medicago sativa | IOMT IIB, chromatography | - |
4.3 |
Medicago sativa | IOMT IIA, chromatography | - |
4.9 |
Organism | Comment | Expression |
---|---|---|
Medicago sativa | treatment of alfalfa cell suspension cultures with elicitor preparations from Saccharomyces cerevisiae or from cell walls of Colletotrichum lindemuthianum results in a ca 200fold induction of isoflavone 0-methyltransferase activity | up |