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Literature summary for 2.1.1.150 extracted from

  • Dalkin, K.; Edwards, R.; Edington, B.; Dixon, R.A.
    Stress responses in alfalfa (Medicago sativa L.). I. Induction of phenylpropanoid biosynthesis and hydrolytic enzymes in elicitor-treated cell suspension cultures (1990), Plant Physiol., 92, 440-446.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol 10-20 mM, up to 39% inhibition Medicago sativa
dithiothreitol 10-20 mM, up to 39% inhibition Medicago sativa

General Stability

General Stability Organism
stabilized in the presence of EDTA and thiol-containing reagents Medicago sativa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme activity was not affected by inclusion of 5 mM Mn2+, Mg2+, or EDTA in the assay mixture Medicago sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 1 * 41000, SDS-PAGE Medicago sativa

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Medicago sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information IOMT II does not catalyse the methylation of apigenin, kaempferol, flavonoids mono-hydroxylated in the 2’,3,4’,5, or 7 positions, flavanones such as naringenin or coumestans such as coumestrol Medicago sativa ?
-
 ?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein. 50% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone i.e. glycitein. 178% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
S-adenosyl-L-methionine + 7,2'-dihydroxy-4'-methoxyisoflavone i.e. 2'-hydroxyformononetin. 55% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 2-hydroxy-7,4'-dimethoxyisoflavone
-
 ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. isoformononetin ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Medicago sativa

Synonyms

Synonyms Comment Organism
IOMT II
-
 Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 Tris buffer Medicago sativa
8.7
-
 glycine-NaOH buffer Medicago sativa

pI Value

Organism Comment pI Value Maximum pI Value
Medicago sativa IOMT IIB, chromatography
-
 4.3
Medicago sativa IOMT IIA, chromatography
-
 4.9

Expression

Organism Comment Expression
Medicago sativa treatment of alfalfa cell suspension cultures with elicitor preparations from Saccharomyces cerevisiae or from cell walls of Colletotrichum lindemuthianum results in a ca 200fold induction of isoflavone 0-methyltransferase activity up