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IUBMB Comments This enzyme, isolated from the bacterium Geobacillus thermodenitrificans , participates in the pathway of tryptophan degradation. The enzyme is part of a system that also includes a bifunctional riboflavin kinase/FMN adenylyltransferase and an FAD reductase, which ensures ample supply of FAD to the monooxygenase.
Synonyms
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FADH2-utilizing monooxygenase
FADH2-utilizing monooxygenase
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FADH2-utilizing monooxygenase
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GTNG_3160
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anthranilate + FADH2 + O2 = 3-hydroxyanthranilate + FAD + H2O
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MetaCyc
anthranilate degradation IV (aerobic)
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anthranilate,FAD:oxygen oxidoreductase (3-hydroxylating)
This enzyme, isolated from the bacterium Geobacillus thermodenitrificans, participates in the pathway of tryptophan degradation. The enzyme is part of a system that also includes a bifunctional riboflavin kinase/FMN adenylyltransferase and an FAD reductase, which ensures ample supply of FAD to the monooxygenase.
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2-hydroxyphenylacetate + FADH2 + O2
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Substrates: 62.79% relative activity
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2-hydroxyphenylacetate + FADH2 + O2
? + FAD + H2O
4-hydroxyphenylacetate + FADH2 + O2
?
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Substrates: 42.7% relative activity
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4-hydroxyphenylacetate + FADH2 + O2
? + FAD + H2O
anthranilate + FADH2 + O2
3-hydroxyanthranilate + FAD
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Substrates: highest activity
?
anthranilate + FADH2 + O2
3-hydroxyanthranilate + FAD + H2O
salicylate + FADH2 + O2
?
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Substrates: 49% relative activity
?
salicylate + FADH2 + O2
? + FAD + H2O
additional information
?
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2-hydroxyphenylacetate + FADH2 + O2
? + FAD + H2O
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Substrates: 67.8% of the activity with anthranilate
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2-hydroxyphenylacetate + FADH2 + O2
? + FAD + H2O
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Substrates: 67.8% of the activity with anthranilate
?
4-hydroxyphenylacetate + FADH2 + O2
? + FAD + H2O
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Substrates: 42.7% of the activity with anthranilate
?
4-hydroxyphenylacetate + FADH2 + O2
? + FAD + H2O
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Substrates: 42.7% of the activity with anthranilate
?
anthranilate + FADH2 + O2
3-hydroxyanthranilate + FAD + H2O
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Substrates: -
?
anthranilate + FADH2 + O2
3-hydroxyanthranilate + FAD + H2O
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Substrates: -
?
salicylate + FADH2 + O2
? + FAD + H2O
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Substrates: 49.6% of the activity with anthranilate
?
salicylate + FADH2 + O2
? + FAD + H2O
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Substrates: 49.6% of the activity with anthranilate
?
additional information
?
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Substrates: no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene
?
additional information
?
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Substrates: no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene
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additional information
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no cofactor: FMNH2
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FADH2
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FADH2
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FADH2-utilizing monooxygenase, no reaction with FMNH2
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151.3
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substrate anthranilate, pH 9.0, 60°C
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9
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9
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assay at, highest activity
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60
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60
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assay at, highest activity
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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HPAH_GEOTN
Geobacillus thermodenitrificans (strain NG80-2)
494
0
56164
Swiss-Prot
other Location (Reliability: 2 )
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using Ni-NTA chromatography
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expressed in Escherichia coli
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expression in Escherichia coli
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transcription level increases highly when anthranilate is used as the sole carbon source
transcription level increases highly when anthranilate is used as the sole carbon source
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transcription level increases highly when anthranilate is used as the sole carbon source
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Liu, X.; Dong, Y.; Li, X.; Ren, Y.; Li, Y.; Wang, W.; Wang, L.; Feng, L.
Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2
Microbiology
156
589-595
2010
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
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