early regulation of genes putatively related to nematode damage-associated molecular pattern recognition (e.g. wall-associated receptor kinases), signalling [nucleotide-binding, leucine-rich repeat (NLRs)], pathogenesis-related (PR) genes (PR1, PR10a), defence-related genes (NB-ARC domain-containing genes), as well as a large number of genes involved in secondary metabolites including diterpenoid biosynthesis (CPS2, OsKSL4, OsKSL10, Oscyp71Z2, oryzalexin synthase, and momilactone A synthase) is observed in Meloidogyne graminicola-resistant mutant line-9. After the nematode juveniles penetrate the roots of line-9, early recognition of invading nematodes triggers plant immune responses mediated by phytoalexins, and other defence proteins such as PR proteins inhibit nematode growth and reproduction
the nematode-resistant T-DNA insertion mutant line-9 shows post-penetration resistance to RRKN and reduced nematode multiplication factor compared to the wild-type JBT 36/14 and a popular basmati rice cultivar Pusa Basmati 1121, quantitative real-time PCR enzyme expression analysis of defense-related plant genes, overview