The enzyme catalyses a net dephosphorylation and oxidation of UMP to generate 5′-dehydrouridine, the first intermediate in the biosynthesis of the unusual aminoribosyl moiety found in several C7-furanosyl nucleosides such as A-90289s, caprazamycins, liposidomycins, muraymycins and FR-900453. Requires Fe2+.
The expected taxonomic range for this enzyme is: unclassified Streptomyces
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SYSTEMATIC NAME
IUBMB Comments
UMP,2-oxoglutarate:oxygen oxidoreductase
The enzyme catalyses a net dephosphorylation and oxidation of UMP to generate 5'-dehydrouridine, the first intermediate in the biosynthesis of the unusual aminoribosyl moiety found in several C7-furanosyl nucleosides such as A-90289s, caprazamycins, liposidomycins, muraymycins and FR-900453. Requires Fe2+.
Substrates: in the absence of the prime substrate UMP, the enzyme is able to catalyze oxidative decarboxylation of 2-oxoglutarate, although at a significantly reduced rate Products: -
Substrates: uridine and UDP, pyruvate, 2-oxoadipate, 2-oxobutyrate, 2-oxovalerate, and oxaloacetate are not recognized as substrates. The enzyme is unable to catalyze dephosphorylation and aldehyde formation using the remaining canonical, monophosphorylated ribo- or deoxyribonucleotides Products: -
Substrates: in the absence of the prime substrate UMP, the enzyme is able to catalyze oxidative decarboxylation of 2-oxoglutarate, although at a significantly reduced rate Products: -
Substrates: uridine and UDP, pyruvate, 2-oxoadipate, 2-oxobutyrate, 2-oxovalerate, and oxaloacetate are not recognized as substrates. The enzyme is unable to catalyze dephosphorylation and aldehyde formation using the remaining canonical, monophosphorylated ribo- or deoxyribonucleotides Products: -
Substrates: in the absence of the prime substrate UMP, the enzyme is able to catalyze oxidative decarboxylation of 2-oxoglutarate, although at a significantly reduced rate Products: -
Substrates: uridine and UDP, pyruvate, 2-oxoadipate, 2-oxobutyrate, 2-oxovalerate, and oxaloacetate are not recognized as substrates. The enzyme is unable to catalyze dephosphorylation and aldehyde formation using the remaining canonical, monophosphorylated ribo- or deoxyribonucleotides Products: -
Substrates: in the absence of the prime substrate UMP, the enzyme is able to catalyze oxidative decarboxylation of 2-oxoglutarate, although at a significantly reduced rate Products: -
Substrates: uridine and UDP, pyruvate, 2-oxoadipate, 2-oxobutyrate, 2-oxovalerate, and oxaloacetate are not recognized as substrates. The enzyme is unable to catalyze dephosphorylation and aldehyde formation using the remaining canonical, monophosphorylated ribo- or deoxyribonucleotides Products: -
Characterization of LipL as a non-heme, Fe(II)-dependent alpha-ketoglutarate:UMP dioxygenase that generates uridine-5-aldehyde during A-90289 biosynthesis
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