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The enzyme appears in viruses and cellular organisms
Synonyms
3-keto-L-gulonate dehydrogenase, 3-ketogulonate dehydrogenase, more
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3-keto-L-gulonate dehydrogenase
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3-ketogulonate dehydrogenase
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3-dehydro-L-gulonate + NAD(P)+ = (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H + H+
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MetaCyc
L-ascorbate degradation II (bacterial, aerobic)
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3-dehydro-L-gulonate:NAD(P)+ 2-oxidoreductase
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(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
3-dehydro-L-gulonate + NAD(P)+
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2,3-diketogulonic acid + NADH + H+
3-dehydro-L-gulonate + NAD+
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r
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(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
3-dehydro-L-gulonate + NAD(P)+
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NADH
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twice effective compared to NADPH
NADPH
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NADH 2fold more effective
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p-chloromercuribenzoate
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32% inhibition at 1 mM, not reversal by GSH
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1.16
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate
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5.2 - 8.4
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50% of maximal activity at pH 5.2 and pH 8.4
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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0°C, 0.03 M Tris-HCl, pH 7.7, 0.62 mg protein/ml, 85% retaining activity in 4 weeks
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Volk, W.A.; Larsen, J.L.
beta-Keto-L-gulonic acid as an intermediate in the bacterial metabolism of ascorbic acid
J. Biol. Chem.
237
2454-2457
1962
Klebsiella aerogenes
brenda
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