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1.14.14.92: benzoate 4-monooxygenase

This is an abbreviated version!
For detailed information about benzoate 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.92

Reaction

benzoate
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
4-hydroxybenzoate
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

An09g03500, benzoate 1,2-dioxygenase, benzoate 4-hydroxylase, benzoate para-hydroxylase, benzoate-4-hydroxylase, benzoate-p-hydroxylase, benzoic 4-hydroxylase, benzoic acid 4-hydroxylase, BphA, Cyp1, CYP53A15, CYP53B1, EC 1.14.13.12, hydroxylase, benzoate 4-, More, oxygenase, benzoate 4-mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.92 benzoate 4-monooxygenase

Cloned

Cloned on EC 1.14.14.92 - benzoate 4-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of benzoate 4-hydroxylase and cytochrome P450 reductase in Aspergillus niger increases enzyme activity
-
gene CYP1f, DNA and amino acid sequence determination and analysis, five introns and six exons, functional expression in Pichia pastoris
gene CYP53B1, functional overexpression in Yarrowia lipolytica strain UOFS Y-2366, multiple copies of CYP53B1 cDNA cloned under control of POX2 promoter, construction of several clones, feeding of stearic acid to the recombinant yeast leads to high accumulation of 4-hydroxybenzoate
-
His-tagged benzoate 4-monooxygenase is expressed in Escherichia coli C43(DE3) cells
PCR amplification, expression in Escherichia coli BL21 (DE3)