Information on EC 3.5.1.24 - choloylglycine hydrolase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.5.1.24
-
RECOMMENDED NAME
GeneOntology No.
choloylglycine hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycocholate + H2O = cholate + glycine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
hydrolysis
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bile acid biosynthesis, neutral pathway
-
-
bile acids degradation
-
-
glycocholate metabolism (bacteria)
-
-
Metabolic pathways
-
-
Primary bile acid biosynthesis
-
-
Secondary bile acid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine amidohydrolase
Also acts on the 3alpha,12alpha-dihydroxy-derivative, and on choloyl-taurine.
CAS REGISTRY NUMBER
COMMENTARY hide
37289-07-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ssp. fragilis ATCC 25285
-
-
Manually annotated by BRENDA team
isolated from bovine rumen
UniProt
Manually annotated by BRENDA team
subsp. lactis, isolated from yoghurt
UniProt
Manually annotated by BRENDA team
subps. animalis , isolated from rat feces
UniProt
Manually annotated by BRENDA team
isolated from stool of breast-fed infant
UniProt
Manually annotated by BRENDA team
isolated from intestine of infant
UniProt
Manually annotated by BRENDA team
Bifidobacterium breve NRRL B-41408
isolated from intestine of infant
UniProt
Manually annotated by BRENDA team
subsp. infantis, isolated from intestine of infant
UniProt
Manually annotated by BRENDA team
subsp. suis, isolated from pig feces
UniProt
Manually annotated by BRENDA team
subsp. longum, isolated from intestine of adult
-
-
Manually annotated by BRENDA team
strain KL412
-
-
Manually annotated by BRENDA team
isolated from sewage
UniProt
Manually annotated by BRENDA team
isolated from sewage
UniProt
Manually annotated by BRENDA team
isolated from sewage
UniProt
Manually annotated by BRENDA team
subsp. pseudolongum, isolated from bovine rumen
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Brevibacillus sp.
-
-
-
Manually annotated by BRENDA team
strain 2308
Uniprot
Manually annotated by BRENDA team
strain 2308
Uniprot
Manually annotated by BRENDA team
activity only detecable in stationary phase cells
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isolated from swine
UniProt
Manually annotated by BRENDA team
activity only detectable in stationary phase cells
-
-
Manually annotated by BRENDA team
strain 21, strain 18
-
-
Manually annotated by BRENDA team
gene bsh
UniProt
Manually annotated by BRENDA team
strain 100-100
-
-
Manually annotated by BRENDA team
ssp. paracasei
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain Lp80
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isolated from chicken
UniProt
Manually annotated by BRENDA team
strain 100-100
-
-
Manually annotated by BRENDA team
strain 100-14
-
-
Manually annotated by BRENDA team
strain 100-93 has no bile salt hydrolase activity
-
-
Manually annotated by BRENDA team
Lactobacillus spp.
49 strains tested
-
-
Manually annotated by BRENDA team
no activity in Bifidobacterium asteroides strain DSM 20089 isolated from hindgut of honeybee
-
-
-
Manually annotated by BRENDA team
no activity in Bifidobacterium coryneforme
-
-
-
Manually annotated by BRENDA team
no activity in Bifidobacterium coryneforme strain DSM 20216 isolated from hindgut of honeybee
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus acidophilus strain CRL 1072
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus casei strain CRL 203
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus delbrueckii strain CRL 454
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus delbrueckii strain CRL 494
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus plantarum strain CRL 573
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus rhamnosus strain NRRL B-442
-
-
-
Manually annotated by BRENDA team
no activity in Lactococcus lactis
-
-
-
Manually annotated by BRENDA team
no activity in Leuconostoc mesenteroides
-
-
-
Manually annotated by BRENDA team
no activity in Streptococcus thermophilus
-
-
-
Manually annotated by BRENDA team
strain CBS 897.97, i.e. Stenotrophomonas maltophilia
-
-
Manually annotated by BRENDA team
strain CBS 897.97, i.e. Stenotrophomonas maltophilia
-
-
Manually annotated by BRENDA team
enzyme is expressed constitutively
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,12-dioxo-5beta-cholanoylglycine + H2O
3,12-dioxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3,12-dioxo-5beta-cholanoyltaurine + H2O
3,12-dioxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3,7-dioxo-5beta-cholanoylglycine + H2O
3,7-dioxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3,7-dioxo-5beta-cholanoyltaurine + H2O
3,7-dioxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3-oxo-5beta-cholanoylglycine + H2O
3-oxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3-oxo-5beta-cholanoyltaurine + H2O
3-oxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oylglycine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyltaurine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholan-24-oylglycine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholanate + glycine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholan-24-oyltaurine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-alpha-aminomethanesulfonic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + aminomethanesulfonic acid
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-beta-Ala + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + beta-Ala
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-DL-Ala + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + DL-Ala
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoylsarcosine + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + sarcosine
show the reaction diagram
-
low activity
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholestan-26-oylglycine + H2O
3alpha,7alpha-dihydroxy-5beta-cholestanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholestan-26-oyltaurine + H2O
3alpha,7alpha-dihydroxy-5beta-cholestanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-homocholanoylglycine + H2O
3alpha,7alpha-dihydroxy-5beta-homocholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-homocholanoyltaurine + H2O
3alpha,7alpha-dihydroxy-5beta-homocholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoylglycine + H2O
3alpha,7beta-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyltaurine + H2O
3alpha,7beta-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-12-oxo-5beta-cholanoylglycine + H2O
3alpha-hydroxy-12-oxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-12-oxo-5beta-cholanoyltaurine + H2O
3alpha-hydroxy-12-oxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-7-oxo-5beta-cholanylglycine + H2O
3alpha-hydroxy-7-oxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-7-oxo-5beta-cholanyltaurine + H2O
3alpha-hydroxy-7-oxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoylglycine + H2O
3beta,7alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoyltaurine + H2O
3beta,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholanoylglycine + H2O
3beta,7beta-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholanoyltaurine + H2O
3beta,7beta-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
cholic acid + H2O
? + glycine
show the reaction diagram
-
-
-
-
?
cholyl-alpha-aminomethanesulfonic acid + H2O
cholic acid + aminomethanesulfonic acid
show the reaction diagram
-
-
-
-
?
cholyl-beta-Ala + H2O
cholic acid + beta-Ala
show the reaction diagram
-
-
-
-
?
cholyl-DL-Ala + H2O
cholic acid + DL-Ala
show the reaction diagram
-
-
-
-
?
cholylsarcosine + H2O
cholic acid + sarcosine
show the reaction diagram
-
low activity
-
-
?
cholyltaurine + H2O
cholic acid + taurine
show the reaction diagram
-
-
-
-
?
glyco-conjugated bile acid + H2O
?
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
glycochenodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
glycocholate + H2O
cholate + glycine
show the reaction diagram
glycocholate + H2O
glycine + cholate
show the reaction diagram
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
show the reaction diagram
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
glycocholic acid + H2O
?
show the reaction diagram
-
-
-
?
glycocholic acid + H2O
cholate + glycine
show the reaction diagram
glycocholic acid + H2O
cholic acid + glycine
show the reaction diagram
glycodeoxycholate + H2O
3alpha,12alpha-dihydroxy-5-beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
show the reaction diagram
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
glycohyodeoxycholate + H2O
glycine + hyodeoxycholate
show the reaction diagram
glycoursodeoxycholate + H2O
glycine + ursodeoxycholate
show the reaction diagram
tauro-conjugated bile acid + H2O
?
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholate + H2O
chenodeoxycholate + taurin
show the reaction diagram
taurochenodeoxycholate + H2O
taurine + chenodeoxycholate
show the reaction diagram
taurochenodeoxycholic acid + H2O
3alpha,12alphadihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
taurocholate + H2O
taurine + cholate
show the reaction diagram
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
taurocholic acid + H2O
cholate + taurine
show the reaction diagram
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
taurodeoxycholic acid + H2O
taurine + deoxycholate
show the reaction diagram
-
-
-
-
?
taurohyodeoxycholic acid + H2O
?
show the reaction diagram
-
-
-
-
?
tauroursodeoxycholic acid + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cholic acid + H2O
? + glycine
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
glycocholate + H2O
cholate + glycine
show the reaction diagram
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
glycocholic acid + H2O
cholate + glycine
show the reaction diagram
J7H3P9
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
taurocholate + H2O
taurine + cholate
show the reaction diagram
Q52UN2
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
taurocholic acid + H2O
cholate + taurine
show the reaction diagram
J7H3P9
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
P54965
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3,7,12-triketo-5beta-cholan-24-oylglycine
-
-
3,7,12-Triketo-5beta-cholanoic acid
-
-
4-amino-phenylacetic acid
-
competitive inhibition
4-chloromercuriacetate
over 98% inhibition at 0.01 mM
-
4-chloromercuriacteate
over 98% inhibition at 0.01 mM
5,5'-dithiobis-2-nitrobenzoic acid
Brevibacillus sp.
-
65% residual activity
AgNO3
Brevibacillus sp.
-
77.3% residual activity
BaCl2
Brevibacillus sp.
-
78.4% residual activity
benzyl penicillin
-
mixed inhibition
Ca2+
91.2% residual activity at 30 mM
caffeic acid phenethyl ester
carnosic acid
-
96.8% inhibition at 5 mM
cholate
-
uncompetitive inhibition of glycocholic acid hydrolysis
cholic acid
chrysophanol
-
3.7% inhibition at 1.25 mM
citraconic anhydride
Brevibacillus sp.
-
33.3% residual activity
demeclocycline
deoxycholic acid
-
competitive inhibition
doxycycline
epicatechin monogallate
erythromycin
-
36.5% inhibition at 5 mM
FeSO4
-
96.1% inhibition at 5 mM
gossypetin
HgCl2
Brevibacillus sp.
-
43.2% residual activity
iodoacetamide
iodoacetate
KCl
-
25.9% inhibition at 5 mM
KI
-
36.8% inhibition at 5 mM
Lincomycin
menadione
methacycline
N-bromosuccinimide
Brevibacillus sp.
-
63.6% residual activity
N-ethylmaleimide
Na2SeO3
-
93.1% inhibition at 5 mM
Na2SO4
-
27.7% inhibition at 5 mM
NaCl
-
27.7% inhibition at 5 mM
NaHCO3
-
20.6% inhibition at 5 mM
NaHIO3
NaI
-
31.8% inhibition at 5 mM
NaIO3
-
86.4% inhibition at 5 mM
-
NiSO4
Brevibacillus sp.
-
92.0% residual activity
Oxytetracycline
periodic acid
phenoxy penicillin
-
mixed inhibition
phenoxyacetic acid
-
competitive inhibition
phenylacetic acid
-
competitive inhibition
Phenylglyoxal
Brevibacillus sp.
-
57.4% residual activity
phenylmethylsulfonyl fluoride
90.9% residual activity at 0.5 mM
PMSF
-
28% inhibition at 2 mM, 67% at 10 mM
Purpurogallin
riboflavin
riboflavin c
-
-
roxarsone
Sodium periodate
-
about 99% inhibition at 0.1-2 mM, complete inhibition at 10 mM
streptomycin
-
-
sulfamethazine
-
48.7% inhibition at 5 mM
taurine
-
uncompetitive inhibition of glycocholic acid hydrolysis
taurocholic acid
-
competitive inhibition of glycocholic acid hydrolysis
theaflavanin
-
18.1% inhibition at 5 mM
-
Trinitrobenzenesulfonic acid
Brevibacillus sp.
-
30.9% residual activity
tylosin
-
23.8% inhibition at 5 mM
vitamin C
-
21.8% inhibition at 5 mM
Woodward's reagent K
Brevibacillus sp.
-
53.6% residual activity
Zn2+
-
5 mM, 69% inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FeSO4
Brevibacillus sp.
-
114.7% residual activity
MgCl2
107.6% activity at 30 mM
MgSO4
112.6% activity at 30 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.028
glycochenodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.26 - 15.04
glycochenodeoxycholic acid
0.022 - 0.024
glycocholate
0.35 - 15.48
glycocholic acid
0.018
glycodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.00308 - 14.12
glycodeoxycholic acid
0.042
taurochenodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.29 - 17.16
taurochenodeoxycholic acid
0.03 - 0.032
taurocholate
0.45 - 37
taurocholic acid
0.049
taurodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.17 - 18.12
taurodeoxycholic acid
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.6 - 83.77
glycochenodeoxycholic acid
44 - 85
glycocholate
8.87 - 82.59
glycocholic acid
6.37 - 632
glycodeoxycholic acid
8.5 - 93.11
taurochenodeoxycholic acid
40 - 76
taurocholate
9.75 - 93.14
taurocholic acid
13.43 - 99.43
taurodeoxycholic acid
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.58 - 36.84
glycochenodeoxycholic acid
4.53 - 5.49
glycocholic acid
3.45 - 6.81
glycodeoxycholic acid
3.36 - 6.28
taurochenodeoxycholic acid
3.04 - 5.85
taurocholic acid
4.21 - 5.49
taurodeoxycholic acid
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.86
4-amino-phenylacetic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
2
benzyl penicillin
-
pH 6.5, 40C, recombinant wild-type enzyme
4.7
cholate
-
pH 8.0, 50C
0.32
cholic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
0.46
deoxycholic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
1.2
phenoxy penicillin
-
pH 6.5, 40C, recombinant wild-type enzyme
0.82
phenoxyacetic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
0.94
phenylacetic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
2.5
taurine
additional information
additional information
-
inhibition kinetics
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.03
Brevibacillus sp.
-
purification step sonification
4
-
pH 5.4, 37C, purified recombinant enzyme, substrate glycodeoxycholic acid
5.6
-
pH 5.4, 37C, purified recombinant enzyme, substrate taurocholic acid
7.7
-
pH 5.4, 37C, purified recombinant enzyme, substrate glycocholic acid
8
-
pH 5.4, 37C, purified recombinant enzyme, substrate taurochenodeoxycholic acid
8.5
-
pH 5.4, 37C, purified recombinant enzyme, substrate taurodeoxycholic acid
12.2
Brevibacillus sp.
-
purification step first Q-Sepharose chromatograpgy
13.8
Brevibacillus sp.
-
purification step second Q-Sepharose chromatograpgy
17.7
-
pH 5.4, 37C, purified recombinant enzyme, substrate glycochenodeoxycholic acid
30
-
pH 6.0, 37 C
33.09
-
immobilized enzyme, pH 6.5, 37C
51.29
-
free enzyme, pH 6.5, 37C
274.2
-
pH 6.0, 37C, recombinant enzyme
5389
-
purified native enzyme
6168
-
purified native enzyme
7904
-
purified native enzyme from strain KL515
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 4.5
-
strain L1 and ATCC 43121, deconjugation of taurocholate
3.8 - 4.5
-
bile salt hydrolase A and B
4 - 5.5
-
strain ATCC 43121, strain 016 and strain L1, deconjugation of glycocholate
4.2 - 4.5
-
bile salt hydrolase A, B, C or D
5 - 6
-
strain O16, deconjugation of taurocholate
5
isozyme BSH A; isozyme BSH C
5.4
-
recombinant enzyme
5.5 - 6
-
isozyme BSH2
5.5 - 7
-
isozyme BSH1, broad optimum
9
Brevibacillus sp.
-
-