Information on EC 3.5.1.24 - choloylglycine hydrolase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
3.5.1.24
-
RECOMMENDED NAME
GeneOntology No.
choloylglycine hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glycocholate + H2O = cholate + glycine
show the reaction diagram
-
-
-
-
glycocholate + H2O = cholate + glycine
show the reaction diagram
substrate binding pocket and active site structure, taurine has a reversed orientation pointing with its sulfo group towards Cys2 and leaving the active site with its amino group ahead, overview
-
glycocholate + H2O = cholate + glycine
show the reaction diagram
substrate specificity and active site structure, residues Cys1, Arg17, Asp20, Asn174, and Arg227 are important for catalysis, Trp21 plays a selective role in binding of bile salt while it suppresses productive binding of substrate analogue penicillin V
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bile acid biosynthesis, neutral pathway
-
-
glycocholate metabolism (bacteria)
-
-
Metabolic pathways
-
-
Primary bile acid biosynthesis
-
-
Secondary bile acid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine amidohydrolase
Also acts on the 3alpha,12alpha-dihydroxy-derivative, and on choloyl-taurine.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
bile salt hydrolase
-
-
-
-
BSH
-
-
-
-
CBAH
-
-
-
-
Conjugated bile acid hydrolase
-
-
-
-
glycocholase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37289-07-9
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ssp. fragilis ATCC 25285
-
-
Manually annotated by BRENDA team
gene bsh, strain ATCC 15705
SwissProt
Manually annotated by BRENDA team
Bifidobacterium animalis subsp. lactis Bi30
-
UniProt
Manually annotated by BRENDA team
strain ATCC 11863
-
-
Manually annotated by BRENDA team
strain ATCC 11863
-
-
Manually annotated by BRENDA team
strains ATCC 15708, KL507, and KL515
-
-
Manually annotated by BRENDA team
Bifidobacterium longum subsp. infantis KL412
strain KL412
-
-
Manually annotated by BRENDA team
Brevibacillus sp.
-
-
-
Manually annotated by BRENDA team
strain 2308
Uniprot
Manually annotated by BRENDA team
strain 2308
Uniprot
Manually annotated by BRENDA team
ATCC 19574, Sinai No. 43-F-4
-
-
Manually annotated by BRENDA team
enzyme is expressed constitutively
-
-
Manually annotated by BRENDA team
activity only detecable in stationary phase cells
-
-
Manually annotated by BRENDA team
enzyme is expressed constitutively
-
-
Manually annotated by BRENDA team
Enterococcus faecium CRL 183
-
-
-
Manually annotated by BRENDA team
human intestinal strain 016, human intestinal strain L1, porcine intestinal strain ATCC 43121
-
-
Manually annotated by BRENDA team
strain NCFM with genes bshA and bshB, and strain PF01
-
-
Manually annotated by BRENDA team
Lactobacillus acidophilus CRL 44
-
-
-
Manually annotated by BRENDA team
activity only detectable in stationary phase cells
-
-
Manually annotated by BRENDA team
strains ATCC 4005 and JCM1069
-
-
Manually annotated by BRENDA team
strains ATCC 4005 and NCIM 2357
-
-
Manually annotated by BRENDA team
ssp. casein
-
-
Manually annotated by BRENDA team
strain 21, strain 18
-
-
Manually annotated by BRENDA team
alpha subunit and beta subunit
Q9F660 and P97038
UniProt
Manually annotated by BRENDA team
strain 100-100, two different antigenic conjugated BSHs, BSHalpha and BSHbeta
-
-
Manually annotated by BRENDA team
Lactobacillus johnsonii 100-100
strain 100-100
-
-
Manually annotated by BRENDA team
ssp. paracasei
-
-
Manually annotated by BRENDA team
strain Lp80
UniProt
Manually annotated by BRENDA team
strain WCFS1 with four bsh genes, and strains CK102 and 80
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum Lp80
strain Lp80
UniProt
Manually annotated by BRENDA team
Lactobacillus reuteri CRL 1098
-
-
-
Manually annotated by BRENDA team
Lactobacillus reuteri CRL 1100
-
-
-
Manually annotated by BRENDA team
Lactobacillus reuteri CRL 1101
-
-
-
Manually annotated by BRENDA team
activity only detectable in stationary phase cells
-
-
Manually annotated by BRENDA team
ssp. salivarius
-
-
Manually annotated by BRENDA team
diverse strains, indigenous isolates from Pakistan, overview
-
-
Manually annotated by BRENDA team
strain 100-100
-
-
Manually annotated by BRENDA team
strain 100-14; strain 100-93 has no bile salt hydrolase activity
-
-
Manually annotated by BRENDA team
Lactobacillus sp. 100-100
strain 100-100
-
-
Manually annotated by BRENDA team
Lactobacillus sp. 100-14
strain 100-14
-
-
Manually annotated by BRENDA team
Lactobacillus sp. 100-93
strain 100-93 has no bile salt hydrolase activity
-
-
Manually annotated by BRENDA team
Lactobacillus spp.
49 strains tested
-
-
Manually annotated by BRENDA team
no activity in Bifidobacterium coryneforme
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus acidophilus strain CRL 1072
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus casei strain CRL 203
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus delbrueckii strain CRL 454
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus delbrueckii strain CRL 494
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus plantarum strain CRL 573
-
-
-
Manually annotated by BRENDA team
no activity in Lactococcus lactis
-
-
-
Manually annotated by BRENDA team
no activity in Leuconostoc mesenteroides
-
-
-
Manually annotated by BRENDA team
no activity in Streptococcus thermophilus
-
-
-
Manually annotated by BRENDA team
strain CBS 897.97, i.e. Stenotrophomonas maltophilia
-
-
Manually annotated by BRENDA team
Stenotrophomonas maltophilia CBS 897.97
strain CBS 897.97, i.e. Stenotrophomonas maltophilia
-
-
Manually annotated by BRENDA team
enzyme is expressed constitutively
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH plays a role in bile tolerance
evolution
-
the BSH from Bifidobactrium longum demonstrates a evolutionary relationship with penicillin V acylase
additional information
-
effect of oral administration to Wistar rats of the immobilized bile salt hydrolase enzyme on serum cholesterol, triglyceride, high density lipoprotein levels and its application in the therapeutic treatment of hypercholesteremia, overview
additional information
-
probiotic organisms colonizing the host gastrointestinal tract need to be bile salt tolerant
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,12-dioxo-5beta-cholanoylglycine + H2O
3,12-dioxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3,12-dioxo-5beta-cholanoyltaurine + H2O
3,12-dioxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3,7-dioxo-5beta-cholanoylglycine + H2O
3,7-dioxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3,7-dioxo-5beta-cholanoyltaurine + H2O
3,7-dioxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3-oxo-5beta-cholanoylglycine + H2O
3-oxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3-oxo-5beta-cholanoyltaurine + H2O
3-oxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oylglycine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyltaurine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholan-24-oylglycine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholanate + glycine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholan-24-oyltaurine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-alpha-aminomethanesulfonic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + aminomethanesulfonic acid
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-beta-Ala + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + beta-Ala
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-DL-Ala + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + DL-Ala
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoylsarcosine + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + sarcosine
show the reaction diagram
-
low activity
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholestan-26-oylglycine + H2O
3alpha,7alpha-dihydroxy-5beta-cholestanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholestan-26-oyltaurine + H2O
3alpha,7alpha-dihydroxy-5beta-cholestanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-homocholanoylglycine + H2O
3alpha,7alpha-dihydroxy-5beta-homocholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-homocholanoyltaurine + H2O
3alpha,7alpha-dihydroxy-5beta-homocholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoylglycine + H2O
3alpha,7beta-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyltaurine + H2O
3alpha,7beta-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-12-oxo-5beta-cholanoylglycine + H2O
3alpha-hydroxy-12-oxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-12-oxo-5beta-cholanoyltaurine + H2O
3alpha-hydroxy-12-oxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-7-oxo-5beta-cholanylglycine + H2O
3alpha-hydroxy-7-oxo-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3alpha-hydroxy-7-oxo-5beta-cholanyltaurine + H2O
3alpha-hydroxy-7-oxo-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoylglycine + H2O
3beta,7alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoyltaurine + H2O
3beta,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholanoylglycine + H2O
3beta,7beta-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholanoyltaurine + H2O
3beta,7beta-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
cholic acid + H2O
? + glycine
show the reaction diagram
-
-
-
-
?
cholyl-alpha-aminomethanesulfonic acid + H2O
cholic acid + aminomethanesulfonic acid
show the reaction diagram
-
-
-
-
?
cholyl-beta-Ala + H2O
cholic acid + beta-Ala
show the reaction diagram
-
-
-
-
?
cholyl-DL-Ala + H2O
cholic acid + DL-Ala
show the reaction diagram
-
-
-
-
?
cholylsarcosine + H2O
cholic acid + sarcosine
show the reaction diagram
-
low activity
-
-
?
cholyltaurine + H2O
cholic acid + taurine
show the reaction diagram
-
-
-
-
?
glyco-conjugated bile acid + H2O
?
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
Stenotrophomonas maltophilia CBS 897.97
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
show the reaction diagram
Bifidobacterium longum subsp. infantis KL412
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
B9V401
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
about 75% activity compared to glycocholic acid
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
-
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
-
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
Q2YS20
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
-
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
-
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
-
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
-
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
Stenotrophomonas maltophilia CBS 897.97
-
-
-
-
?
glycocholate + H2O
glycine + cholate
show the reaction diagram
Bifidobacterium longum subsp. infantis KL412
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
G0YYC2
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
strain CK102
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
Lactobacillus sp. 100-100
-
-
-
-
?
glycocholic acid + H2O
?
show the reaction diagram
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
show the reaction diagram
B9V401
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
activity assay
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
show the reaction diagram
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
show the reaction diagram
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
show the reaction diagram
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
show the reaction diagram
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
show the reaction diagram
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
show the reaction diagram
Stenotrophomonas maltophilia CBS 897.97
-
-
-
-
?
glycodeoxycholate + H2O
3alpha,12alpha-dihydroxy-5-beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
B9V401
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
show the reaction diagram
Lactobacillus sp. 100-100
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
G0YYC2
about 80% activity compared to glycocholic acid
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
-
isoform BSH1 shows much higher hydrolysis on glycodeoxycholic acid than isoforms BSH2, BSH3 and BSH4
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
Lactobacillus reuteri CRL 1100, Lactobacillus reuteri CRL 1098, Enterococcus faecium CRL 183, Lactobacillus acidophilus CRL 44, Lactobacillus reuteri CRL 1101
-
best substrate
-
-
?
glycohyodeoxycholate + H2O
glycine + hyodeoxycholate
show the reaction diagram
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia CBS 897.97
-
-
-
-
?
tauro-conjugated bile acid + H2O
?
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholate + H2O
taurine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholate + H2O
taurine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholate + H2O
taurine + chenodeoxycholate
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
Lactobacillus sp. 100-100
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
B9V401
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
about 15% activity compared to glycocholic acid
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,12alphadihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholate + H2O
taurine + cholate
show the reaction diagram
-
-
-
-
?
taurocholate + H2O
taurine + cholate
show the reaction diagram
-
-
-
-
?
taurocholate + H2O
taurine + cholate
show the reaction diagram
-
-
-
-
?
taurocholate + H2O
taurine + cholate
show the reaction diagram
Q52UN2
-
-
-
?
taurocholate + H2O
taurine + cholate
show the reaction diagram
-
deconjugation
-
-
?
taurocholate + H2O
taurine + cholate
show the reaction diagram
Bifidobacterium longum subsp. infantis KL412
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
B9V401
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
hydrolyzing activity against tauroconjugated bile salts, but not glycoconjugated bile salts
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
about 20% activity compared to glycocholic acid
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
Lactobacillus sp. 100-100
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
P54965
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
Bifidobacterium longum subsp. infantis KL412
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
B9V401
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
show the reaction diagram
Lactobacillus sp. 100-100
-
-
-
-
?
taurodeoxycholic acid + H2O
taurine + deoxycholate
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
G0YYC2
less than 20% activity compared to glycocholic acid
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
Lactobacillus reuteri CRL 1100, Lactobacillus reuteri CRL 1098, Enterococcus faecium CRL 183, Lactobacillus acidophilus CRL 44, Lactobacillus reuteri CRL 1101
-
-
-
-
?
taurohyodeoxycholic acid + H2O
?
show the reaction diagram
-
-
-
-
?
tauroursodeoxycholic acid + H2O
?
show the reaction diagram
-
-
-
-
?
glycoursodeoxycholate + H2O
glycine + ursodeoxycholate
show the reaction diagram
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia CBS 897.97
-
-
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
-
additional information
?
-
Lactobacillus spp.
-
enzyme activity is important for Lactobacillus colonization of human intestine
-
?
additional information
?
-
-
the enzyme catalyzes the deconjugation of glycine- or taurine-linked bile salts
-
-
-
additional information
?
-
Q2YS20
the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts
-
-
-
additional information
?
-
-
substrate specificity profile, overview
-
-
-
additional information
?
-
-
catalyzes the deconjugation of conjugated bile acids to liberate free primary bile acids and amino acids
-
-
-
additional information
?
-
Brevibacillus sp.
-
catalyzes the hydrolysis of glycine- and taurine-conjugated bile salts to amino acid residues and free bile salts
-
-
-
additional information
?
-
-
BSH can hydrolyze all the six major human bile salts and at least two animal bile salts. BSH from five strains show a better deconjugation rate on glycine-conjugated bile salts than on taurine-conjugated forms
-
-
-
additional information
?
-
-
BSH catalyzes the hydrolysis of amide bond in conjugated bile salts and free amino acids are released, which form the deconjugated bile acid, mainly cholic and quenodeoxycholic
-
-
-
additional information
?
-
-
oxgall is a substrate for the enzyme from different strains, overview
-
-
-
additional information
?
-
-
strain JCM1069 exhibits hydrolase activity against the taurodeoxycholic acid but not against the taurocholic acid, although both acids have taurine as their amino acid moiety but vary in their steroid moieties at 7alpha position
-
-
-
additional information
?
-
Brevibacillus sp.
-
the enzyme hydrolyzes all of the six major human bile salts
-
-
-
additional information
?
-
-
BSHs BSHs is highly substrate-specific and can identify its substrate, bile acids, on amino acid groups, glycine/taurine, and also on cholate steroid nucleus. BSH recognizes the cholate group
-
-
-
additional information
?
-
-
BSHs is highly substrate-specific and can identify its substrate, bile acids, on amino acid groups, glycine/taurine, and also on cholate steroid nucleus. BSH recognizes the cholate group. No activity with taurocholic acid
-
-
-
additional information
?
-
-
BSHs is highly substrate-specific, the two isozymes, encoded by two bsh genes, show different substrate specificities. BSHs can identify its substrate, bile acids, on amino acid groups, glycine/taurine, and also on cholate steroid nucleus. BSH recognizes the cholate group. The recombinant BSH from strain PF01 is active with tauroconjugated bile salts, but not with glycoconjugated bile slats
-
-
-
additional information
?
-
-
the SH group in the N-terminal cysteine is responsible for BSH activity, and amino acids, viz. Asp20, Tyr82, Asn175, and Arg228, are believed to take part actively along with Cys in catalysis of bile salts
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
additional information
?
-
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms
-
-
-
additional information
?
-
Bifidobacterium longum subsp. infantis KL412
-
substrate specificity, overview
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cholic acid + H2O
? + glycine
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
about 75% activity compared to glycocholic acid
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
G0YYC2
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
strain CK102
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
show the reaction diagram
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
G0YYC2
about 80% activity compared to glycocholic acid
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
-
isoform BSH1 shows much higher hydrolysis on glycodeoxycholic acid than isoforms BSH2, BSH3 and BSH4
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
about 15% activity compared to glycocholic acid
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurocholate + H2O
taurine + cholate
show the reaction diagram
Q52UN2
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
about 20% activity compared to glycocholic acid
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
show the reaction diagram
P54965
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
G0YYC2
less than 20% activity compared to glycocholic acid
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
show the reaction diagram
Lactobacillus reuteri CRL 1100, Lactobacillus reuteri CRL 1098, Enterococcus faecium CRL 183, Lactobacillus acidophilus CRL 44, Lactobacillus reuteri CRL 1101
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
show the reaction diagram
Lactobacillus reuteri CRL 1100, Lactobacillus reuteri CRL 1098, Enterococcus faecium CRL 183, Lactobacillus acidophilus CRL 44, Lactobacillus reuteri CRL 1101
-
best substrate
-
-
?
additional information
?
-
Lactobacillus spp.
-
enzyme activity is important for Lactobacillus colonization of human intestine
-
?
additional information
?
-
-
the enzyme catalyzes the deconjugation of glycine- or taurine-linked bile salts
-
-
-
additional information
?
-
Q2YS20
the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts
-
-
-
additional information
?
-
-
catalyzes the deconjugation of conjugated bile acids to liberate free primary bile acids and amino acids
-
-
-
additional information
?
-
Brevibacillus sp.
-
catalyzes the hydrolysis of glycine- and taurine-conjugated bile salts to amino acid residues and free bile salts
-
-
-
additional information
?
-
-
BSH can hydrolyze all the six major human bile salts and at least two animal bile salts. BSH from five strains show a better deconjugation rate on glycine-conjugated bile salts than on taurine-conjugated forms
-
-
-
additional information
?
-
-
BSH catalyzes the hydrolysis of amide bond in conjugated bile salts and free amino acids are released, which form the deconjugated bile acid, mainly cholic and quenodeoxycholic
-
-
-
additional information
?
-
-
oxgall is a substrate for the enzyme from different strains, overview
-
-
-
additional information
?
-
-
strain JCM1069 exhibits hydrolase activity against the taurodeoxycholic acid but not against the taurocholic acid, although both acids have taurine as their amino acid moiety but vary in their steroid moieties at 7alpha position
-
-
-
additional information
?
-
Brevibacillus sp.
-
the enzyme hydrolyzes all of the six major human bile salts
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
additional information
?
-
Bifidobacterium animalis subsp. lactis, Bifidobacterium animalis subsp. lactis Bi30
G0YYC2
the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3,7,12-triketo-5beta-cholan-24-oylglycine
-
-
3,7,12-Triketo-5beta-cholanoic acid
-
-
4-amino-phenylacetic acid
-
competitive inhibition
5,5'-dithiobis-2-nitrobenzoic acid
Brevibacillus sp.
-
65% residual activity
AgNO3
Brevibacillus sp.
-
77.3% residual activity
BaCl2
Brevibacillus sp.
-
78.4% residual activity
benzyl penicillin
-
mixed inhibition
Ca2+
G0YYC2
91.2% residual activity at 30 mM
cholate
-
uncompetitive inhibition of glycocholic acid hydrolysis
cholic acid
-
competitive product inhibition
cholic acid
-
competitive inhibition
citraconic anhydride
Brevibacillus sp.
-
33.3% residual activity
CoCl2
Brevibacillus sp.
-
90.9% residual activity
Cu2+
-
0.3 mM, 45% inhibition
Cu2+
G0YYC2
1.4% residual activity at 5 mM
CuSO4
Brevibacillus sp.
-
0% residual activity
deoxycholic acid
-
competitive inhibition
EDTA
Brevibacillus sp.
-
80.69% residual activity
EDTA
G0YYC2
75.7% residual activity at 30 mM
Hg2+
-
0.0001 mM, 67% inhibition
Hg2+
G0YYC2
2.2% residual activity at 0.2 mM
HgCl2
Brevibacillus sp.
-
43.2% residual activity
iodoacetamide
G0YYC2
75.8% residual activity at 30 mM
iodoacetate
-
strong inhibition
MgSO4
Brevibacillus sp.
-
87.5% residual activity
N-bromosuccinimide
Brevibacillus sp.
-
63.6% residual activity
NiSO4
Brevibacillus sp.
-
92.0% residual activity
periodic acid
-
strong inhibition
periodic acid
G0YYC2
0.6% residual activity at 3 mM
phenoxy penicillin
-
mixed inhibition
phenoxyacetic acid
-
competitive inhibition
phenylacetic acid
-
competitive inhibition
Phenylglyoxal
Brevibacillus sp.
-
57.4% residual activity
phenylmethylsulfonyl fluoride
G0YYC2
90.9% residual activity at 0.5 mM
taurine
-
uncompetitive inhibition of glycocholic acid hydrolysis
taurocholic acid
-
competitive inhibition of glycocholic acid hydrolysis
Trinitrobenzenesulfonic acid
Brevibacillus sp.
-
30.9% residual activity
Woodward's reagent K
Brevibacillus sp.
-
53.6% residual activity
Zn2+
-
5 mM, 69% inhibition
ZnSO4
Brevibacillus sp.
-
76.1% residual activity
MnSO4
Brevibacillus sp.
-
95.5% residual activity
additional information
-
BSH is vigorously inhibited by thiol enzyme inhibitors
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FeSO4
Brevibacillus sp.
-
114.7% residual activity
MgCl2
G0YYC2
107.6% activity at 30 mM
MgSO4
G0YYC2
112.6% activity at 30 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.028
glycochenodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.26
glycochenodeoxycholic acid
-
-
14
glycochenodeoxycholic acid
-
-
0.022
glycocholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.024
glycocholate
-
pH 6.5, 40C, recombinant mutant T2A
0.35
glycocholic acid
-
-
0.5
glycocholic acid
-
pH 5.8-6.3, temperature not specified in the publication
3.6
glycocholic acid
-
-
8
glycocholic acid
-
-
0.018
Glycodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.00308
glycodeoxycholic acid
Brevibacillus sp.
-
apparent value, calculated from a Lineweaver-Burk plot
0.00308
glycodeoxycholic acid
Brevibacillus sp.
-
pH not specified in the publication, temperature not specified in the publication
0.2
glycodeoxycholic acid
-
-
0.042
taurochenodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.29
taurochenodeoxycholic acid
-
-
3
taurochenodeoxycholic acid
-
-
0.03
taurocholate
-
pH 6.5, 40C, recombinant mutant T2A
0.032
taurocholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.45
taurocholic acid
-
-
0.76
taurocholic acid
-
hydrolase A
0.95
taurocholic acid
-
hydrolase B
37
taurocholic acid
-
-
0.049
taurodeoxycholate
-
pH 6.5, 40C, recombinant wild-type enzyme
0.17
taurodeoxycholic acid
-
-
3.5
taurodeoxycholic acid
-
-
1.2
glycodeoxycholic acid
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Michaelis-Menten kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
44
glycocholate
-
pH 6.5, 40C, recombinant mutant T2A
85
glycocholate
-
pH 6.5, 40C, recombinant wild-type enzyme
632
glycodeoxycholic acid
Brevibacillus sp.
-
apparent value, calculated from a Lineweaver-Burk plot
632
glycodeoxycholic acid
Brevibacillus sp.
-
pH not specified in the publication, temperature not specified in the publication
40
taurocholate
-
pH 6.5, 40C, recombinant mutant T2A
76
taurocholate
-
pH 6.5, 40C, recombinant wild-type enzyme
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.86
4-amino-phenylacetic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
2
benzyl penicillin
-
pH 6.5, 40C, recombinant wild-type enzyme
4.7
cholate
-
pH 8.0, 50C
0.32
cholic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
1.2
phenoxy penicillin
-
pH 6.5, 40C, recombinant wild-type enzyme
0.82
phenoxyacetic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
0.94
phenylacetic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
2.5
taurine
-
pH 8.0, 50C
2.5
taurocholic acid
-
pH 8.0, 50C
0.46
deoxycholic acid
-
pH 6.5, 40C, recombinant wild-type enzyme
additional information
additional information
-
inhibition kinetics
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1.03
Brevibacillus sp.
-
purification step sonification
12.2
Brevibacillus sp.
-
purification step first Q-Sepharose chromatograpgy
13.8
Brevibacillus sp.
-
purification step second Q-Sepharose chromatograpgy
30
-
pH 6.0, 37 C
33.09
-
immobilized enzyme, pH 6.5, 37C
51.29
-
free enzyme, pH 6.5, 37C
5389
-
purified native enzyme
6168
-
purified native enzyme
7904
-
purified native enzyme from strain KL515
additional information
-
-
additional information
-
-
additional information
Brevibacillus sp.
-
glycocholic acid 2.2 U, glycodeoxycholic acid 2.7 U, glycochenodeoxycholic acid 1.7 U, taurocholic acid 1.7 U, taurodeoxycholic acid 1.7 U, taurochenodeoxycholic acid 1.7 U
additional information
-
bile salt hydrolase activity 1.0 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information
-
bile salt hydrolase activity 1.1 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information
-
bile salt hydrolase activity 1.0 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information
-
BSH activity 1.9 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information
-
BSH activity in different strains, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.5 - 4.5
-
strain L1 and ATCC 43121, deconjugation of taurocholate
3.8 - 4.5
-
bile salt hydrolase A and B
4 - 5.5
-
strain ATCC 43121, strain 016 and strain L1, deconjugation of glycocholate
4.2 - 4.5
-
bile salt hydrolase A, B, C or D
5 - 6
-
strain O16, deconjugation of taurocholate
6.5
Q52UN2
assay at
6.5
Brevibacillus sp.
-
bile salt hydrolase assay
6.5
B9V401
activity assay
6.5
Brevibacillus sp.
-
activity assay
7 - 8
Q2YS20
-
9
Brevibacillus sp.
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3 - 11
-
activity range, profile overview
4.7 - 6.5
G0YYC2
about 50% activity at pH 4.7 and 6.5
6 - 9
Q2YS20
activity range
6 - 9
Brevibacillus sp.
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
no activity at 5C and 60C, thermal inactivation of enzyme is irreversible
37
Q2YS20
assay at
37
B9V401
activity assay
40
Brevibacillus sp.
-
bile salt hydrolase assay
40
Brevibacillus sp.
-
activity assay
60
Brevibacillus sp.
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 60
-
activity range, profile overview
40 - 65
Brevibacillus sp.
-
-
45 - 55
G0YYC2
85% and 82% of maximum activity at 45C and 55C, respectively
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.45
-
isoelectric focusing
4.45
-
BSH type B
4.65
-
isoelectric focusing, enzymes from strains KL507 and KL515
4.65
-
isoelectric focusing
4.65
-
BSH types A and C
4.9
G0YYC2
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
activity detected only in stationary-phase cells
Manually annotated by BRENDA team
-
optimization of culture conditions for the production of bile salt hydrolase, overview. Porduction is highest in sucrose- and peptone-grown cells
Manually annotated by BRENDA team
Lactobacillus sp. 100-100
-
activity detected only in stationary-phase cells
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Lactobacillus johnsonii 100-100
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Clostridium perfringens (strain 13 / Type A)
Clostridium perfringens (strain 13 / Type A)
Clostridium perfringens (strain 13 / Type A)
Clostridium perfringens (strain 13 / Type A)
Clostridium perfringens (strain 13 / Type A)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
28000
Brevibacillus sp.
-
monomer, determined by SDS-PAGE
697838
28200
Brevibacillus sp.
-
monomer, determined by MALDI-TOF analysis
697838
56000
Brevibacillus sp.
-
native protein, determined by gel filtration
697838
80000
-
bile salt hydrolase D, nondenaturing PAGE
209041
95000
-
bile salt hydrolase C, nondenaturing PAGE
209041
105000
-
bile salt hydrolase B, nondenaturing PAGE
209041
115000
-
bile salt hydrolase A, nondenaturing PAGE
209041
120000 - 140000
G0YYC2
gel filtration
720209
125000 - 150000
-
-
710913
126000
-
strain O16, L1, and ATCC 43121, gel filtration
209046
140000
-
-
680269
146000
-
gel filtration
649238
250000
-
gel filtration
209038
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 32500, SDS-PAGE
?
-
x * 37000, BSH from strain CK102
?
-
x * 36000, isoform BSH4, SDS-PAGE, x * 37000, isoform BSH1, SDS-PAGE, x * 37000, isoform BSH3, SDS-PAGE, x * 39000, isoform BSH2, SDS-PAGE
?
Bifidobacterium longum subsp. infantis KL412
-
x * 35000, SDS-PAGE
-
?
-
x * 36000, isoform BSH4, SDS-PAGE, x * 37000, isoform BSH1, SDS-PAGE, x * 37000, isoform BSH3, SDS-PAGE, x * 39000, isoform BSH2, SDS-PAGE
-
homodimer
Brevibacillus sp.
-
-
homodimer
Brevibacillus sp.
-
2 * 56000
homotetramer
-
-
homotetramer
G0YYC2
4 * 35000, SDS-PAGE
homotetramer
-
4 * 36500, SDS-PAGE
tetramer
-
secondary structure, the enzyme possesses an alphabetabetaalpha tetra-lamellar tertiary structure arrangement, crystal structure analysis
tetramer
-
the enzyme contains an N-terminal thiol hydrolase activity site, quarternary structure, crystal structure analysis, overview
tetramer
-
4 * 35042
trimer
-
beta3, bile salt hydrolase D, 3 * 38000, SDS-PAGE, alpha3, 3 * 42000, bile salt hydrolase A, SDS-PAGE, alpha1,beta2, bile salt hydrolase C, 1 * 42000 + 2 * 38000, SDS-PAGE, alpha2beta1, bile salt hydrolase B, 3 * 38000, SDS-PAGE
trimer
-
BSHalpha and BSHbeta are combined to form native homo- and heterotrimers
trimer
Lactobacillus johnsonii 100-100
-
beta3, bile salt hydrolase D, 3 * 38000, SDS-PAGE, alpha3, 3 * 42000, bile salt hydrolase A, SDS-PAGE, alpha1,beta2, bile salt hydrolase C, 1 * 42000 + 2 * 38000, SDS-PAGE, alpha2beta1, bile salt hydrolase B, 3 * 38000, SDS-PAGE
-
homotetramer
Bifidobacterium animalis subsp. lactis Bi30
-
4 * 35000, SDS-PAGE
-
additional information
-
computational structure comparisons
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
proteolytic modification
-
the catalytic nucleophil residue has no role in zymogen processing into its corresponding active form, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type and mutant enzymes, X-ray diffraction structure determination and analysis at 2.3-2.5 A resolution, molecular replacement
-
enzyme in complex with substrate taurodeoxycholate and as apoenzyme, complexed enzyme by hanging drop vapour diffusion method, 18C, mixing of 0.003 ml protein solution and 0.002 ml reservoir solution, the latter containing 2.6 M ammonium sulfate, 0.1 M sodium citrate, pH 6.0, and 1 mM taurodeoxycholate, 3 days, apoenzyme crystals by sitting drop vapour diffusion method, 18C, mixing of 0.0015 ml protein solution with 0.001 ml reservoir solution containing 25% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M Bis-Tris, pH 5.5, 3 days, orthorhombic crystals in both cases, X-ray diffraction structure determination and analysis at 1.7 and 2.1 A resolution, respectively
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55
-
75 min, completely stable
670898
60 - 70
G0YYC2
a significant decrease in enzyme activity is observed at temperatures higher than 60C. At 70C, 90% of the catalytic activity is lost
720209
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, pH 7.0, stable for 8 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose column chromatography and Sephacryl S-300 gel filtration
G0YYC2
native enzyme 33.5fold from strain ATCC 11863 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration
-
native enzyme from strains ATCC 15708, KL507, and KL515 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration, 30.3fold from strain KL515
-
recombinant enzyme expressed in Escherichia coli
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
native enzyme 21.6fold from strain KL412 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration
-
purified to homogeneity using Q-Sepharose chromatography
Brevibacillus sp.
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, and polymyxin B resin chromatography, the latter to eliminate lipopolysaccharide contamination
Q2YS20
nickel-based affinity chromatography
-
recombinant BSH from strain PF01 purifed from recombinant Escherichia coli strrain BL21(DE3)
-
bile acid hydrolase A, B, C and D
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain DH5alpha intracellularly in absence of bile salts
Q52UN2
expressed in Escherichia coli XL1 Blue cells
G0YYC2
expression in Escherichia coli
-
gene bsh, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
genes bsh are cloned and expressed in Escherichia coli
-
gene cgh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3)
Q2YS20
DNA and amino acid sequence determination, analysis, and comparison of BSH from strain PF01, expression in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
-
gene bsh, DNA and amino acid sequence determination and analysis, real-time PCR expression analysis
-
expressed in Escherichia coli BL21(DE3) cells
-
vector-free engineering by chromosomal integration of an exogenous cbh gene, encoding the conjugated BSH, is cloned and expressed in Lactobacillus casei strain LK1 with the aid of pSMA23-derived vectors
-
into pCR-Blunt and subsequently into pNZ8048 for heterologous overexpression of BSH in Lactococcus lactis cells
B9V401
expression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
rapid induction of BSH expression by bile
-
BSH is upregulated by bile salts in the growth medium
-
rapid induction of BSH expression by bile
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
Lactobacillus spp.
-
enzyme activity and resistance to toxicity of bile salts are unrelated, but enzyme activity is important for Lactobacillus colonization of human intestine
C1A
-
site-directed mutagenesis, residue Cys1 is situated at the tip of strand beta1, inactive mutant, structure analysis in comparison to the wild-type enzyme
additional information
-
substitution of the N-terminal Cys with Ser or Thr results in an inactive enzyme, which emphasizes the importance of the presence of cysteine. The enzyme is immobilized by micro-encapsulation showing low acid resistance
T2A
-
site-directed mutagenesis, the mutant shows reduced activity and kcat, structure analysis in comparison to the wild-type enzyme
additional information
Q2YS20
construction of an isogenic deletion mutant strain DELTAcgh, the growth of the the cgh mutant is inhibted by additon of 5% or 10% bile acid, but is similar to the wild-type strain in absence of bile acids, the mutant enzyme does not confer resistance to bile acids to the organism
additional information
-
knockout of gene bshA gene of strain NCFM decreases the ability to hydrolyze the chenodeoxycholic containing bile salts, e.g., taurochenodeoxycholic acid and glycochenodeoxycholic acid
additional information
-
BSH from ATCC 4005 is immobilized in 0.5% gellan gum gel, method, overview
additional information
-
BSH from strain ATCC 4005 is immobilized on 0.5% gellan gum gel for potential economic utilization
additional information
-
BSH is microencapsulated by foodgrade whey protein-gum arabic, comparison to living Lactobacillus plantarum WCFS1 in vitro, endogenously producing BSH, for its catalytic efficacy. BSH efficacy is better against pancreatin and low gastric pH initially, but its activity decreases further due to proteolytic degradation, whereas WCFS1 cells withstands such conditions. Bile salt deconjugation rates of microencapsulated BSH overproducing cells of strain 80 are 0.0049 mmol/g microcapsule per h towards glycoconjugates and 0.00079 mmol/g microcapsule per h towards tauroconjugates in the simulated gastro-intestine
additional information
-
the enzyme is immobilized by micro-encapsulation. The microcapsules metabolize glyco- and tauroconjugated bile salts at rates of 0.0102 and 0.00185 mmol/g microcapsule per hour, respectively, and show better acid resistance. They exhibit an improved deconjugation with 49.4% of glycoconjugates per h in a simulated human gastrointestinal model and complete deconjugation after 4 h
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
Brevibacillus sp.
-
using bile salt deconjugation to lower serum cholesterol levels in hypercholesteremic patients and prevent hypercholesteremia in normal people is of great interest
medicine
-
protein encapsulation is an excellent tool to protect enzymes during transit through gastric conditions and release enzyme activity in the proximal small intestine
medicine
-
effect of oral administration to Wistar rats of the immobilized bile salt hydrolase enzyme on serum cholesterol, triglyceride, high density lipoprotein levels and its application in the therapeutic treatment of hypercholesteremia, overview
medicine
-
protein encapsulation is an excellent tool to protect enzymes during transit through gastric conditions and release enzyme activity in the proximal small intestine
medicine
-
cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity