Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.24 extracted from

  • Jarocki, P.
    Molecular characterization of bile salt hydrolase from Bifidobacterium animalis subsp. lactis Bi30 (2011), J. Microbiol. Biotechnol., 21, 838-845.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
MgCl2 107.6% activity at 30 mM Bifidobacterium animalis subsp. lactis
MgSO4 112.6% activity at 30 mM Bifidobacterium animalis subsp. lactis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1 Blue cells Bifidobacterium animalis subsp. lactis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 91.2% residual activity at 30 mM Bifidobacterium animalis subsp. lactis
Cu2+ 1.4% residual activity at 5 mM Bifidobacterium animalis subsp. lactis
EDTA 75.7% residual activity at 30 mM Bifidobacterium animalis subsp. lactis
Hg2+ 2.2% residual activity at 0.2 mM Bifidobacterium animalis subsp. lactis
iodoacetamide 75.8% residual activity at 30 mM Bifidobacterium animalis subsp. lactis
periodic acid 0.6% residual activity at 3 mM Bifidobacterium animalis subsp. lactis
phenylmethylsulfonyl fluoride 90.9% residual activity at 0.5 mM Bifidobacterium animalis subsp. lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
4 * 35000, SDS-PAGE Bifidobacterium animalis subsp. lactis
120000 140000 gel filtration Bifidobacterium animalis subsp. lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycochenodeoxycholic acid + H2O Bifidobacterium animalis subsp. lactis about 75% activity compared to glycocholic acid chenodeoxycholate + glycine
-
?
glycochenodeoxycholic acid + H2O Bifidobacterium animalis subsp. lactis Bi30 about 75% activity compared to glycocholic acid chenodeoxycholate + glycine
-
?
glycocholic acid + H2O Bifidobacterium animalis subsp. lactis 100% activity 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
?
glycocholic acid + H2O Bifidobacterium animalis subsp. lactis Bi30 100% activity 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
?
glycodeoxycholic acid + H2O Bifidobacterium animalis subsp. lactis about 80% activity compared to glycocholic acid deoxycholate + glycine
-
?
additional information Bifidobacterium animalis subsp. lactis the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms ?
-
?
additional information Bifidobacterium animalis subsp. lactis Bi30 the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms ?
-
?
taurochenodeoxycholic acid + H2O Bifidobacterium animalis subsp. lactis about 15% activity compared to glycocholic acid chenodeoxycholate + taurine
-
?
taurochenodeoxycholic acid + H2O Bifidobacterium animalis subsp. lactis Bi30 about 15% activity compared to glycocholic acid chenodeoxycholate + taurine
-
?
taurocholic acid + H2O Bifidobacterium animalis subsp. lactis about 20% activity compared to glycocholic acid 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
?
taurocholic acid + H2O Bifidobacterium animalis subsp. lactis Bi30 about 20% activity compared to glycocholic acid 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
?
taurodeoxycholic acid + H2O Bifidobacterium animalis subsp. lactis less than 20% activity compared to glycocholic acid deoxycholate + taurine
-
?

Organism

Organism UniProt Comment Textmining
Bifidobacterium animalis subsp. lactis G0YYC2
-
-
Bifidobacterium animalis subsp. lactis Bi30 G0YYC2
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Sephacryl S-300 gel filtration Bifidobacterium animalis subsp. lactis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycochenodeoxycholic acid + H2O about 75% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis chenodeoxycholate + glycine
-
?
glycochenodeoxycholic acid + H2O about 75% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis Bi30 chenodeoxycholate + glycine
-
?
glycocholic acid + H2O 100% activity Bifidobacterium animalis subsp. lactis 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
?
glycocholic acid + H2O 100% activity Bifidobacterium animalis subsp. lactis Bi30 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
?
glycodeoxycholic acid + H2O about 80% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis deoxycholate + glycine
-
?
additional information the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms Bifidobacterium animalis subsp. lactis ?
-
?
additional information the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms Bifidobacterium animalis subsp. lactis Bi30 ?
-
?
taurochenodeoxycholic acid + H2O about 15% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis chenodeoxycholate + taurine
-
?
taurochenodeoxycholic acid + H2O about 15% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis Bi30 chenodeoxycholate + taurine
-
?
taurocholic acid + H2O about 20% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
?
taurocholic acid + H2O about 20% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis Bi30 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
?
taurodeoxycholic acid + H2O less than 20% activity compared to glycocholic acid Bifidobacterium animalis subsp. lactis deoxycholate + taurine
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, SDS-PAGE Bifidobacterium animalis subsp. lactis

Synonyms

Synonyms Comment Organism
bile salt hydrolase
-
Bifidobacterium animalis subsp. lactis
BSH
-
Bifidobacterium animalis subsp. lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
around 50°C Bifidobacterium animalis subsp. lactis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 55 85% and 82% of maximum activity at 45°C and 55°C, respectively Bifidobacterium animalis subsp. lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 a significant decrease in enzyme activity is observed at temperatures higher than 60°C. At 70°C, 90% of the catalytic activity is lost Bifidobacterium animalis subsp. lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
-
Bifidobacterium animalis subsp. lactis

pH Range

pH Minimum pH Maximum Comment Organism
4.7 6.5 about 50% activity at pH 4.7 and 6.5 Bifidobacterium animalis subsp. lactis

pI Value

Organism Comment pI Value Maximum pI Value
Bifidobacterium animalis subsp. lactis isoelectric focusing
-
4.9