Activating Compound | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 107.6% activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
MgSO4 | 112.6% activity at 30 mM | Bifidobacterium animalis subsp. lactis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1 Blue cells | Bifidobacterium animalis subsp. lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 91.2% residual activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
Cu2+ | 1.4% residual activity at 5 mM | Bifidobacterium animalis subsp. lactis | |
EDTA | 75.7% residual activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
Hg2+ | 2.2% residual activity at 0.2 mM | Bifidobacterium animalis subsp. lactis | |
iodoacetamide | 75.8% residual activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
periodic acid | 0.6% residual activity at 3 mM | Bifidobacterium animalis subsp. lactis | |
phenylmethylsulfonyl fluoride | 90.9% residual activity at 0.5 mM | Bifidobacterium animalis subsp. lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Bifidobacterium animalis subsp. lactis |
120000 | 140000 | gel filtration | Bifidobacterium animalis subsp. lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 75% activity compared to glycocholic acid | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | about 75% activity compared to glycocholic acid | chenodeoxycholate + glycine | - |
? | |
glycocholic acid + H2O | Bifidobacterium animalis subsp. lactis | 100% activity | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
glycocholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | 100% activity | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
glycodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 80% activity compared to glycocholic acid | deoxycholate + glycine | - |
? | |
additional information | Bifidobacterium animalis subsp. lactis | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | ? | - |
? | |
additional information | Bifidobacterium animalis subsp. lactis Bi30 | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | ? | - |
? | |
taurochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 15% activity compared to glycocholic acid | chenodeoxycholate + taurine | - |
? | |
taurochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | about 15% activity compared to glycocholic acid | chenodeoxycholate + taurine | - |
? | |
taurocholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 20% activity compared to glycocholic acid | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
taurocholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | about 20% activity compared to glycocholic acid | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
taurodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | less than 20% activity compared to glycocholic acid | deoxycholate + taurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium animalis subsp. lactis | G0YYC2 | - |
- |
Bifidobacterium animalis subsp. lactis Bi30 | G0YYC2 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Sephacryl S-300 gel filtration | Bifidobacterium animalis subsp. lactis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycochenodeoxycholic acid + H2O | about 75% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | chenodeoxycholate + glycine | - |
? | |
glycochenodeoxycholic acid + H2O | about 75% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis Bi30 | chenodeoxycholate + glycine | - |
? | |
glycocholic acid + H2O | 100% activity | Bifidobacterium animalis subsp. lactis | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
glycocholic acid + H2O | 100% activity | Bifidobacterium animalis subsp. lactis Bi30 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
glycodeoxycholic acid + H2O | about 80% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | deoxycholate + glycine | - |
? | |
additional information | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | Bifidobacterium animalis subsp. lactis | ? | - |
? | |
additional information | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | Bifidobacterium animalis subsp. lactis Bi30 | ? | - |
? | |
taurochenodeoxycholic acid + H2O | about 15% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | chenodeoxycholate + taurine | - |
? | |
taurochenodeoxycholic acid + H2O | about 15% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis Bi30 | chenodeoxycholate + taurine | - |
? | |
taurocholic acid + H2O | about 20% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
taurocholic acid + H2O | about 20% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis Bi30 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
taurodeoxycholic acid + H2O | less than 20% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | deoxycholate + taurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, SDS-PAGE | Bifidobacterium animalis subsp. lactis |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Bifidobacterium animalis subsp. lactis |
BSH | - |
Bifidobacterium animalis subsp. lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
around 50°C | Bifidobacterium animalis subsp. lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | 85% and 82% of maximum activity at 45°C and 55°C, respectively | Bifidobacterium animalis subsp. lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | a significant decrease in enzyme activity is observed at temperatures higher than 60°C. At 70°C, 90% of the catalytic activity is lost | Bifidobacterium animalis subsp. lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | - |
- |
Bifidobacterium animalis subsp. lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | 6.5 | about 50% activity at pH 4.7 and 6.5 | Bifidobacterium animalis subsp. lactis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bifidobacterium animalis subsp. lactis | isoelectric focusing | - |
4.9 |