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Literature summary for 3.5.1.24 extracted from

  • Delpino, M.V.; Marchesini, M.I.; Estein, S.M.; Comerci, D.J.; Cassataro, J.; Fossati, C.A.; Baldi, P.C.
    A bile salt hydrolase of Brucella abortus contributes to the establishment of a successful infection through the oral route in mice (2007), Infect. Immun., 75, 299-305.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene cgh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3) Brucella abortus

Protein Variants

Protein Variants Comment Organism
additional information construction of an isogenic deletion mutant strain DELTAcgh, the growth of the the cgh mutant is inhibited by addition of 5% or 10% bile acid, but is similar to the wild-type strain in absence of bile acids, the mutant enzyme does not confer resistance to bile acids to the organism Brucella abortus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Brucella abortus the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts ?
-
 ?
additional information Brucella abortus 2308 the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts ?
-
 ?

Organism

Organism UniProt Comment Textmining
Brucella abortus Q2YS20
-
-
Brucella abortus 2308 Q2YS20
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, and polymyxin B resin chromatography, the latter to eliminate lipopolysaccharide contamination Brucella abortus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycocholate + H2O
-
 Brucella abortus glycine + cholate
-
 ?
glycocholate + H2O
-
 Brucella abortus 2308 glycine + cholate
-
 ?
additional information the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts Brucella abortus ?
-
 ?
additional information the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts Brucella abortus 2308 ?
-
 ?

Synonyms

Synonyms Comment Organism
bile salt hydrolase
-
 Brucella abortus
CGH
-
 Brucella abortus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Brucella abortus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Brucella abortus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity range Brucella abortus