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Literature summary for 3.5.1.24 extracted from

  • Kumar, R.S.; Brannigan, J.A.; Prabhune, A.A.; Pundle, A.V.; Dodson, G.G.; Dodson, E.J.; Suresh, C.G.
    Structural and functional analysis of a conjugated bile salt hydrolase from Bifidobacterium longum reveals an evolutionary relationship with penicillin V acylase (2006), J. Biol. Chem., 281, 32516-32525.
    View publication on PubMed
Search for more literature for 3.5.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene bsh, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Bifidobacterium longum

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and mutant enzymes, X-ray diffraction structure determination and analysis at 2.3-2.5 A resolution, molecular replacement Bifidobacterium longum

Protein Variants

Protein Variants Comment Organism
C1A site-directed mutagenesis, residue Cys1 is situated at the tip of strand beta1, inactive mutant, structure analysis in comparison to the wild-type enzyme Bifidobacterium longum
T2A site-directed mutagenesis, the mutant shows reduced activity and kcat, structure analysis in comparison to the wild-type enzyme Bifidobacterium longum

Inhibitors

Inhibitors Comment Organism Structure
4-amino-phenylacetic acid competitive inhibition Bifidobacterium longum
benzyl penicillin mixed inhibition Bifidobacterium longum
cholic acid competitive inhibition Bifidobacterium longum
deoxycholic acid competitive inhibition Bifidobacterium longum
phenoxy penicillin mixed inhibition Bifidobacterium longum
phenoxyacetic acid competitive inhibition Bifidobacterium longum
phenylacetic acid competitive inhibition Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Bifidobacterium longum
0.018
-
 glycodeoxycholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.022
-
 glycocholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.024
-
 glycocholate pH 6.5, 40°C, recombinant mutant T2A Bifidobacterium longum
0.028
-
 glycochenodeoxycholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.03
-
 taurocholate pH 6.5, 40°C, recombinant mutant T2A Bifidobacterium longum
0.032
-
 taurocholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.042
-
 taurochenodeoxycholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.049
-
 taurodeoxycholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bifidobacterium longum the enzyme catalyzes the deconjugation of glycine- or taurine-linked bile salts ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the catalytic nucleophil residue has no role in zymogen processing into its corresponding active form, overview Bifidobacterium longum

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) Bifidobacterium longum

Reaction

Reaction Comment Organism Reaction ID
glycocholate + H2O = cholate + glycine substrate specificity and active site structure, residues Cys1, Arg17, Asp20, Asn174, and Arg227 are important for catalysis, Trp21 plays a selective role in binding of bile salt while it suppresses productive binding of substrate analogue penicillin V Bifidobacterium longum
a

Source Tissue

Source Tissue Comment Organism Textmining
small intestine microflora Bifidobacterium longum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycochenodeoxycholate + H2O
-
 Bifidobacterium longum glycine + chenodeoxycholate
-
 ?
glycocholate + H2O
-
 Bifidobacterium longum glycine + cholate
-
 ?
glycodeoxycholate + H2O
-
 Bifidobacterium longum glycine + deoxycholate
-
 ?
additional information the enzyme catalyzes the deconjugation of glycine- or taurine-linked bile salts Bifidobacterium longum ?
-
 ?
additional information substrate specificity profile, overview Bifidobacterium longum ?
-
 ?
taurochenodeoxycholate + H2O
-
 Bifidobacterium longum taurine + chenodeoxycholate
-
 ?
taurocholate + H2O
-
 Bifidobacterium longum taurine + cholate
-
 ?
taurodeoxycholate + H2O
-
 Bifidobacterium longum taurine + deoxycholate
-
 ?

Subunits

Subunits Comment Organism
tetramer secondary structure, the enzyme possesses an alphabetabetaalpha tetra-lamellar tertiary structure arrangement, crystal structure analysis Bifidobacterium longum

Synonyms

Synonyms Comment Organism
BSH
-
 Bifidobacterium longum
conjugated bile salt hydrolase
-
 Bifidobacterium longum
More the enzyme is a member of N-terminal nucleophil hydrolase structural superfamily Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Bifidobacterium longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
 taurocholate pH 6.5, 40°C, recombinant mutant T2A Bifidobacterium longum
44
-
 glycocholate pH 6.5, 40°C, recombinant mutant T2A Bifidobacterium longum
76
-
 taurocholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
85
-
 glycocholate pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Bifidobacterium longum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Bifidobacterium longum
0.32
-
 cholic acid pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.46
-
 deoxycholic acid pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.82
-
 phenoxyacetic acid pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.86
-
 4-amino-phenylacetic acid pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
0.94
-
 phenylacetic acid pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
1.2
-
 phenoxy penicillin pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum
2
-
 benzyl penicillin pH 6.5, 40°C, recombinant wild-type enzyme Bifidobacterium longum