Information on EC 2.7.12.1 - dual-specificity kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
2.7.12.1
-
RECOMMENDED NAME
GeneOntology No.
dual-specificity kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (Ser/Thr- and Tyr-phosphorylating)
This family of enzymes can phosphorylate both Ser/Thr and Tyr residues.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biliverdin reductase
-
-
biliverdin reductase
-
human biliverdin reductase is a dual-specificity kinase (Ser/Thr and Tyr)
cdc2/CDC28-like protein kinase
P49759
-
Clik1
-
-
-
-
Clk/Sty protein kinase
P22518
-
DPK
Q84U74, Q8GV28, Q8GV29, Q8GV30
-
dual specificity protein kinase
Q7XJ65
-
dual specificity protein kinase TTK
P33981
-
dual specificity protein kinase TTK
P35761
-
dual specificity tyrosine phosphorylated and regulated kinase 1B
-
-
dual specificity tyrosine phosphorylated and regulated kinase 1B
Q9Z188
-
dual specificity tyrosine phosphorylation regulated kinase 1A
-
-
dual-specificity protein kinase
-
-
dual-specificity protein kinase
Q84U74, Q8GV28, Q8GV29, Q8GV30
-
dual-specificity tyrosine phosphorylation-regulated kinase
-
-
dual-specificity tyrosine phosphorylation-regulated kinase 1A
-
-
dual-specificity tyrosine-phosphorylation regulated kinase 1A
Q13627
-
dual-specificity tyrosine-phosphorylation regulated kinase 1A
-
-
dual-specificity tyrosine-phosphorylation regulated kinase 1A
Q61214
-
dual-specificity tyrosine-phosphorylation regulated kinase 1A
Q63470
-
dual-specificity tyrosine-phosphorylation regulated kinase 1B
Q9Y463
-
dual-specificity tyrosine-phosphorylation regulated kinase 2
Q92630
-
dual-specificity tyrosine-phosphorylation regulated kinase 3
O43781
-
dual-specificity tyrosine-phosphorylation-regulated kinase
-
-
dual-specificity tyrosine-phosphorylation-regulated kinase 2
-
-
dual-specificity tyrosine-regulated kinase
-
-
dual-specificity tyrosine-regulated kinase 1A
-
-
DYRK
Q13627
-
DYRK
Q63470
-
DYRK1A
-
-
DYRK1A
-
; isoform
DYRK1A
Q61214
-
DYRK1A
Q63470
-
Dyrk1A kinase
-
-
DYRK1B
-
-
DYRK1B
-
-
DYRK1B
-
isoform
DYRK1B
Q9Y463
-
DYRK1B
Q9Z188
-
DYRK2
-
-
DYRK3
Q922Y0
-
Esk kinase
P35761
-
minibrain kinase/dual-specificity tyrosine phosphorylated and regulated kinase 1A
-
-
non-receptor tyrosine kinase spore lysis A
P18160
-
protein kinase AFC1
P51566
-
protein kinase AFC2
P51567
-
protein kinase AFC3
P51568
-
protein kinase CLK1
P49759
-
protein kinase CLK1
P22518
-
protein kinase CLK2
P49760
-
protein kinase CLK2
O35491
-
protein kinase CLK3
P49761
-
protein kinase CLK3
O35492
-
protein kinase CLK3
Q63117
-
protein kinase CLK4
O35493
-
protein kinase Doa
P49762
-
protein kinase gene DYRK3
O43781
-
protein kinase KNS1
P32350
-
protein kinase lkh1
Q10156
-
protein kinase YAK1
P14680
-
protein tyrosine kinase 2
-
-
Rad53 protein kinase
P22216
-
receptor serine/threonine/tyrosine kinase 1
-
-
Ser/Thr/Tyr kinase
Mycobacterium bovis AF2
-
-
-
Ser/Thr/Tyr kinase
-
-
Ser/Thr/Tyr kinase
-
-
-
serine/threonine protein kinase minibrain
P49657
-
serine/threonine protein kinase MPS1
P54199
-
serine/threonine/tyrosine kinase
-
-
serine/threonine/tyrosine protein kinase
-
-
STY protein
P22518
-
STY protein kinase
-
-
STYK
Mycobacterium bovis AF2
-
-
-
STYK1/NOK
-
-
TTK
P33981
-
Yak1p protein kinase
P14680
-
MNB protein
Q13627
-
additional information
-
the enzyme belongs to the insulin receptor substrate family
CAS REGISTRY NUMBER
COMMENTARY
134549-83-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ATCC 27405
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
adult patients with Down syndrome
SwissProt
Manually annotated by BRENDA team
at least two splicing variants
-
-
Manually annotated by BRENDA team
Homo sapiens; adult individuals with Down syndrome
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
at least two splicing variants
SwissProt
Manually annotated by BRENDA team
wild-type and transgenic mice, C57BL/6J strain, EpoR-HM mice
SwissProt
Manually annotated by BRENDA team
wild-type and transgenic mice, mice heterozygous for a Dyrk1A targeted mutation (Dyrk1A+/2), maintained in a C57BL/6J-129Ola (C57-129) mixed genetic background
SwissProt
Manually annotated by BRENDA team
Mycobacterium bovis AF2
-
-
-
Manually annotated by BRENDA team
strain CDC1551 and H37Rv
-
-
Manually annotated by BRENDA team
strain CDC1551 and H37Rv
-
-
Manually annotated by BRENDA team
strain PCC 7120
-
-
Manually annotated by BRENDA team
isozyme DPK1; ssp. japonica, cv. Jiucaiqing, four isozymes DPK1-DPK4
SwissProt
Manually annotated by BRENDA team
isozyme DPK2; ssp. japonica, cv. Jiucaiqing, four isozymes DPK1-DPK4
SwissProt
Manually annotated by BRENDA team
isozyme DPK4; ssp. japonica, cv. Jiucaiqing, four isozymes DPK1-DPK4
SwissProt
Manually annotated by BRENDA team
isozyme DPPK3; ssp. japonica, cv. Jiucaiqing, four isozymes DPK1-DPK4
SwissProt
Manually annotated by BRENDA team
Thermospora curvatum
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
overexpressed DYRK1A contributes to neurofibrillary degeneration in Down syndrome more significantly than in subjects with two copies of the DYRK1A gene and sporadic Alzheimers disease
malfunction
-
Dyrk1A participates in the pathogenic mechanisms underlying the mental and other physical symptoms of Down syndrome, alterations in Dyrk1A expression are frequently associated with Down syndrome phenotypes. Knock-out mice are embryonic lethal and heterozygous Dyrk1A mice exhibit decreased neonatal viability, developmental delay, and altered neocortical pyramidal cells
malfunction
-
dyrk1b antisense morpholino knockdown animals reveal modestly expanded expression of the dorsal axial mesoderm marker gsc while the pan-mesodermal marker bik is largely unaffected, show moderately reduced endodermal markers cas and sox17, and reduced expression of the pharyngeal pouch marker edn1
malfunction
-
knockdown of DYRK1A in cortical neurons disrupts neuritogenesis and alters microtubule stability
malfunction
-
heterozygous mice for Dyrk1A show marked alterations in traction ability, prehensile reflex and balance, but only present a slight impairment of visuo-spatial memory even though they show a robust decrease of CA1, CA2, CA3 and dentate gyrus cells
physiological function
-
human biliverdin reductase is an upstream activator of the insulin/insulin growth factor-1 and mitogen-activated protein kinase signaling pathways, and also a basic-leucine-zipper DNA/chromatin-binding transcription factor, an activator and anchor protein for translocation of protein kinase C betaII and zeta isozymes within cell compartments, and a kinase kinase for their activation. Biliverdin reductase is essential for mitogen-activated protein kinase-extracellular signal-regulated kinase 1/2-eukaryotic-like protein kinase signaling and has been identified as the cytoplasm-nuclear heme transporter of extracellular signal-regulated kinase 1/2 and hematin
physiological function
-
the zebrafish dyrk1b gene is important for endoderm formation, craniofacial patterning, and ndr2 expression
physiological function
-
DYRK1A primes S1388, a glycogen synthase kinase 3beta phosphorylation sites on microtubule associated protein 1B
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + amphiphysin I
ADP + phosphorylated amphiphysin I
show the reaction diagram
-
-
-
-
?
ATP + casein
ADP + phosphorylated casein
show the reaction diagram
-
-
-
-
?
ATP + caspase-9
ADP + phosphorylated caspase-9
show the reaction diagram
-
-
-
-
?
ATP + CREB
ADP + phosphorylated CREB
show the reaction diagram
-
-
-
-
?
ATP + cyclin L2
ADP + phosphorylated cyclin L2
show the reaction diagram
-
-
-
-
?
ATP + dynamin 1
ADP + phosphorylated dynamin 1
show the reaction diagram
-
-
-
-
?
ATP + dynamin I
ADP + phosphorylated dynamin I
show the reaction diagram
-
-
-
-
?
ATP + eIF2Bepsilon
ADP + phosphorylated eIF2Bepsilon
show the reaction diagram
-
-
-
-
?
ATP + FKHR
ADP + phosphorylated FKHR
show the reaction diagram
-
-
-
-
?
ATP + Gli1 protein
ADP + phosphorylated Gli1 protein
show the reaction diagram
-
-
-
-
?
ATP + glycogen synthase
ADP + phosphorylated glycogen synthase
show the reaction diagram
-
-
-
-
?
ATP + histone
ADP + phosphorylated histone
show the reaction diagram
-
recombinant glutathione S-transferase-Dyrk/fusion protein catalyzes histone phosphorylation on tyrosine and Ser/Thr residues
-
-
?
ATP + histone H1
ADP + phosphorylated histone H1
show the reaction diagram
-
-
-
-
?
ATP + insulin receptor kinase substrate 1
ADP + phosphorylated insulin receptor kinase substrate 1
show the reaction diagram
-
phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway, phosphorylation at serine residues leading to blockage of insulin action, overview
-
-
?
ATP + microtubule associated protein 1B
ADP + phosphorylated microtubule associated protein 1B
show the reaction diagram
-
DYRK1A phosphorylates the S1392 site on microtubule associated protein 1B
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
-
-
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
-
phosphorylation on a C-terminal Ser residue
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
P35761
phosphorylation on serine, threonine, and tyrosine residues
-
-
?
ATP + neurodegeneration-related septin 4
ADP + phosphorylated neurodegeneration-related septin 4
show the reaction diagram
-
SEPT4, also known as Pnutl2/CDCrel-2
-
-
?
ATP + NFAT
ADP + phosphorylated NFAT
show the reaction diagram
-
-
-
-
?
ATP + Notch protein
ADP + phosphorylated Notch protein
show the reaction diagram
-
-
-
-
?
ATP + peptide DYRKtide
ADP + phosphorylated peptide DYRKtide
show the reaction diagram
-
synthetic peptide substrate
-
-
?
ATP + peptide DYRKtide
ADP + phosphorylated peptide DYRKtide
show the reaction diagram
Q9Z188
synthetic peptide substrate
-
-
?
ATP + poly-(Tyr-Glu)
ADP + phospho-poly-(Tyr-Glu)
show the reaction diagram
-
tyrosine kinase substrate
-
-
?
ATP + Pop2p
ADP + phosphorylated Pop2p
show the reaction diagram
P14680
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
O35491, O35492, O35493
autophosphorylation
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
autophosphorylation
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
Q63470
autophosphorylation
-
-
-
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
autophosphorylation
-
-
-
ATP + protein
ADP + phosphoprotein
show the reaction diagram
Q92630
autophosphorylation
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
autophosphorylation
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
the enzyme phosphorylates proteins on serine, threonine, and tyrosine
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
Rad53 autophosphorylation activity depends on trans phosphorylation mediated by Mec1 and does not require physical association with other proteins
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
P49760
autophosphorylates on Ser/Thr and Tyr residues
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
autophosphorylates on Ser/Thr and Tyr residues
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
autophosphorylation on Tyr residues
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
Q63117
recombinant glutathione S-transferase-Dyrk fusion protein catalyzed autophosphorylation on tyrosine and serine/threonine residues
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
Q63470
when expressed in E. coli the enzyme catalyzes autophosphorylation on Tyr residues
-
-
-
ATP + protein
ADP + phosphoprotein
show the reaction diagram
Q92630
when expressed in E. coli the enzyme catalyzes autophosphorylation on Tyr residues
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
P35761
autophosphorylation on serine, threonine, and tyrosine residues
-
-
-
ATP + protein
ADP + phosphoprotein
show the reaction diagram
P33981
can phosphorylate serine, threonine, and tyrosine hydroxyamino acids
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
-
kinase can phosphorylate serine, threonine and tyrosine residues
-
-
?
ATP + protein tyrosine
ADP + protein tyrosine phosphate
show the reaction diagram
P18160
autophosphorylated on Ser and Thr residues
-
-
?
ATP + Ser/Arg-rich splicing factors
ADP + phosphorylated Ser/Arg-rich splicing factor
show the reaction diagram
P22518
-
-
-
?
ATP + SF3B1 protein-L-Thr434
ADP + [SF3B1 protein]-L-Thr434 phosphate
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 2
ADP + phosphorylated splicing factor 2
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 3
ADP + phosphorylated splicing factor 3
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 3B1
ADP + phosphorylated splicing factor 3B1
show the reaction diagram
-
splicing factor 3B1 is poshorylated at Thr434
-
-
?
ATP + SR protein
ADP + hyperphosphorylated SR protein
show the reaction diagram
O35491, O35492, O35493
i.e. serine-rich and arginine-rich proteins
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
-
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
Q61214
-
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
Q9Y463
enzyme is involved in the regulation of nuclear functions
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
DYRK may be involved in the abnormal neurogenesis found in Down syndrome
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
functions as a negative regulator of the cell cycle in Saccharomyces cerevisiae, acting downstream of the cAMP-dependent protein kinase
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
might be a component of a signaling pathway regulating nuclear functions
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
enzyme may be involved in cell cycle control
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
P49657
the enzyme is required in distinct neuroblast proliferation centers during postembryonic neurogenesis
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
enzyme is a good candidate to mediate some of the pleiotropic effects of Down syndrome
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
Yak1 acts downstream from, or on a parallel pathway to, the kinase step in the Ras/cAMP pathway
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
MNB protein may play a significant role in a signaling pathway regulating nuclear functions of neuronal cell proliferation, contributing to certain features of Down syndrome
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
Q63117
enzyme regulates a predominately testicular function
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
P14680
Yak1p and Pop2p are part of a novel glucose-sensing system in yeast that is involved in growth control in response to glucose availability
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
O35491, O35492, O35493
enzyme may be constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
P22518
the enzyme phosphorylates SR splicing factors and regulates their intranuclear distribution
-
-
?
ATP + STAT3
ADP + phosphorylated STAT3
show the reaction diagram
-
-
-
-
?
ATP + synaptojanin I
ADP + phosphorylated synaptojanin I
show the reaction diagram
-
-
-
-
?
ATP + tau protein-L-Thr434
ADP + [tau protein]-L-Thr434 phosphate
show the reaction diagram
-
-
-
-
?
biliverdin + ?
bilirubin + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
autophosphorylation
-
-
-
additional information
?
-
-
activity is dependent on tyrosine residues between subdomains VII and VIII
-
-
-
additional information
?
-
Q63117
a glutathione S-transferase fusion protein of Clk3 catalyzes autophosphorylation of the kinase but not phosphorylation of the exogenous substrates histone or casein
-
-
-
additional information
?
-
-
the enzyme activates STE12-dependent processes in Saccharomyces cerevisiae
-
-
-
additional information
?
-
-
a role for tyrosine phosphorylation in controlling Dictyostelium development
-
-
-
additional information
?
-
P18160
regulates the differentiation of spore cells
-
-
-
additional information
?
-
-
required for the execution of checkpoint arrest at multiple stages of the cell cycle. Rad53 modulates the lagging strand replication apparatus by controlling phosphorylation of the DNA polymerase alpha-primase complex in response to intra-S DNA damage
-
-
-
additional information
?
-
P22216
Rad53 exerts its role in checkpoint control through regulation of the Polo kinase Cdc5
-
-
-
additional information
?
-
-
the SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast
-
-
-
additional information
?
-
-
the enzyme is not essential for cell growth and a variety of other cellular processes in yeast
-
-
-
additional information
?
-
P32350
negative regulation of filamentous growth and flocculation
-
-
-
additional information
?
-
P35761
Esk kinase may play some role in the control of cell proliferation or differentiation
-
-
-
additional information
?
-
P33981
the enzyme is associated with cell proliferation
-
-
-
additional information
?
-
-
may function in a checkpoint control which couples DNA replication to mitosis. The level of the RPK1 transcript is extremely low and constant throughout the mitotic cycle. However it is regulated during cellular differentiation, being decreased in alpha-factor-treated cells and increased late in meiosis in alpha diploids. Rpk1 is involved in a pathway that coordinates cell proliferation and differentiation
-
-
-
additional information
?
-
-
the enzyme is associated with nitrate dissimilation involving the NirV domain
-
-
-
additional information
?
-
-
the enzyme is developmentally regulated
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of Mg-chelatase and chlorophyll biosynthesis
-
-
-
additional information
?
-
Q84U74, Q8GV28, Q8GV29, Q8GV30
the enzyme is involved in response to plant abiotic and biotic stresses
-
-
-
additional information
?
-
-
the enzyme of strain PCC 7120 contains a pectinesterase domain catalyzing the hydrolysis of pectin, the enzyme probably interacts with chaperonic proteins via tetratrico peptide repeats TPR
-
-
-
additional information
?
-
-
the enzyme performs autophosphorylation at Thr202, Tyr72 and Tyr83, the enzyme also shows biliverdin reductase activity
-
-
-
additional information
?
-
-
the enzyme probably interacts with chaperonic proteins via tetratrico peptide repeats TPR
-
-
-
additional information
?
-
-
DYRK1A-mediated phosphorylation of the beta-amyloid (Ab) precursor protein APP
-
-
-
additional information
?
-
Q922Y0
DYRK3 attenuates red cell production selectively during anemia
-
-
-
additional information
?
-
Q13627
increased dosage of Dyrk1A in brains of individuals with Down syndrome (DS) correlates to increased level of 3R-tau protein, abnormal hyperphosphorylation and aggregation of tau proteins, leads to neurofibrillary degeneration in Down syndrome
-
-
-
additional information
?
-
Q13627
participation in regulating tau phosphorylation, provides a novel mechanism leading to neurofibrillary pathology in Down syndrome
-
-
-
additional information
?
-
-
phosphorylation of viral protein E7 of human papillomvirus HPV16, DYRK1A increases the transforming potential of HPV16-infected cells because of the greater stability of phosphorylated HPV16E7
-
-
-
additional information
?
-
Q61214
role of Dyrk1A in cognitive phenotypes of monosomy 21
-
-
-
additional information
?
-
-
Dyrk1A undergoes self-activation through autophosphorylation at Tyr 321
-
-
-
additional information
?
-
-
neither clathrin heavy chain nor endophilin 1 is phosphorylated by the Dyrk1A
-
-
-
additional information
?
-
-
the enzyme probably interacts with chaperonic proteins via tetratrico peptide repeats TPR
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + amphiphysin I
ADP + phosphorylated amphiphysin I
show the reaction diagram
-
-
-
-
?
ATP + caspase-9
ADP + phosphorylated caspase-9
show the reaction diagram
-
-
-
-
?
ATP + CREB
ADP + phosphorylated CREB
show the reaction diagram
-
-
-
-
?
ATP + cyclin L2
ADP + phosphorylated cyclin L2
show the reaction diagram
-
-
-
-
?
ATP + dynamin 1
ADP + phosphorylated dynamin 1
show the reaction diagram
-
-
-
-
?
ATP + dynamin I
ADP + phosphorylated dynamin I
show the reaction diagram
-
-
-
-
?
ATP + eIF2Bepsilon
ADP + phosphorylated eIF2Bepsilon
show the reaction diagram
-
-
-
-
?
ATP + FKHR
ADP + phosphorylated FKHR
show the reaction diagram
-
-
-
-
?
ATP + Gli1 protein
ADP + phosphorylated Gli1 protein
show the reaction diagram
-
-
-
-
?
ATP + glycogen synthase
ADP + phosphorylated glycogen synthase
show the reaction diagram
-
-
-
-
?
ATP + insulin receptor kinase substrate 1
ADP + phosphorylated insulin receptor kinase substrate 1
show the reaction diagram
-
phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway
-
-
?
ATP + microtubule associated protein 1B
ADP + phosphorylated microtubule associated protein 1B
show the reaction diagram
-
DYRK1A phosphorylates the S1392 site on microtubule associated protein 1B
-
-
?
ATP + neurodegeneration-related septin 4
ADP + phosphorylated neurodegeneration-related septin 4
show the reaction diagram
-
SEPT4, also known as Pnutl2/CDCrel-2
-
-
?
ATP + NFAT
ADP + phosphorylated NFAT
show the reaction diagram
-
-
-
-
?
ATP + Notch protein
ADP + phosphorylated Notch protein
show the reaction diagram
-
-
-
-
?
ATP + SF3B1 protein-L-Thr434
ADP + [SF3B1 protein]-L-Thr434 phosphate
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 2
ADP + phosphorylated splicing factor 2
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 3
ADP + phosphorylated splicing factor 3
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 3B1
ADP + phosphorylated splicing factor 3B1
show the reaction diagram
-
splicing factor 3B1 is poshorylated at Thr434
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
-
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
Q61214
-
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
Q9Y463
enzyme is involved in the regulation of nuclear functions
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
DYRK may be involved in the abnormal neurogenesis found in Down syndrome
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
functions as a negative regulator of the cell cycle in Saccharomyces cerevisiae, acting downstream of the cAMP-dependent protein kinase
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
might be a component of a signaling pathway regulating nuclear functions
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
enzyme may be involved in cell cycle control
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
P49657
the enzyme is required in distinct neuroblast proliferation centers during postembryonic neurogenesis
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
enzyme is a good candidate to mediate some of the pleiotropic effects of Down syndrome
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
Yak1 acts downstream from, or on a parallel pathway to, the kinase step in the Ras/cAMP pathway
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
-
MNB protein may play a significant role in a signaling pathway regulating nuclear functions of neuronal cell proliferation, contributing to certain features of Down syndrome
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
Q63117
enzyme regulates a predominately testicular function
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
P14680
Yak1p and Pop2p are part of a novel glucose-sensing system in yeast that is involved in growth control in response to glucose availability
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
O35491, O35492, O35493
enzyme may be constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
P22518
the enzyme phosphorylates SR splicing factors and regulates their intranuclear distribution
-
-
?
ATP + STAT3
ADP + phosphorylated STAT3
show the reaction diagram
-
-
-
-
?
ATP + synaptojanin I
ADP + phosphorylated synaptojanin I
show the reaction diagram
-
-
-
-
?
ATP + tau protein-L-Thr434
ADP + [tau protein]-L-Thr434 phosphate
show the reaction diagram
-
-
-
-
?
biliverdin + ?
bilirubin + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
a role for tyrosine phosphorylation in controlling Dictyostelium development
-
-
-
additional information
?
-
P18160
regulates the differentiation of spore cells
-
-
-
additional information
?
-
-
required for the execution of checkpoint arrest at multiple stages of the cell cycle. Rad53 modulates the lagging strand replication apparatus by controlling phosphorylation of the DNA polymerase alpha-primase complex in response to intra-S DNA damage
-
-
-
additional information
?
-
P22216
Rad53 exerts its role in checkpoint control through regulation of the Polo kinase Cdc5
-
-
-
additional information
?
-
-
the SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast
-
-
-
additional information
?
-
-
the enzyme is not essential for cell growth and a variety of other cellular processes in yeast
-
-
-
additional information
?
-
P32350
negative regulation of filamentous growth and flocculation
-
-
-
additional information
?
-
P35761
Esk kinase may play some role in the control of cell proliferation or differentiation
-
-
-
additional information
?
-
P33981
the enzyme is associated with cell proliferation
-
-
-
additional information
?
-
-
may function in a checkpoint control which couples DNA replication to mitosis. The level of the RPK1 transcript is extremely low and constant throughout the mitotic cycle. However it is regulated during cellular differentiation, being decreased in alpha-factor-treated cells and increased late in meiosis in alpha diploids. Rpk1 is involved in a pathway that coordinates cell proliferation and differentiation
-
-
-
additional information
?
-
-
the enzyme is associated with nitrate dissimilation involving the NirV domain
-
-
-
additional information
?
-
-
the enzyme is developmentally regulated
-
-
-
additional information
?
-
-
the enzyme is involved in regulation of Mg-chelatase and chlorophyll biosynthesis
-
-
-
additional information
?
-
Q84U74, Q8GV28, Q8GV29, Q8GV30
the enzyme is involved in response to plant abiotic and biotic stresses
-
-
-
additional information
?
-
-
Dyrk1A undergoes self-activation through autophosphorylation at Tyr 321
-
-
-
additional information
?
-
-
neither clathrin heavy chain nor endophilin 1 is phosphorylated by the Dyrk1A
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ATP
-
binding site residue Tyr317 is important
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
-
Mg2+
-
-
Mg2+
Q9Z188
-
Mg2+
-
required for kinase activity
Mn2+
-
-
Mn2+
Q9Z188
-
Mn2+
-
essential for autophosposphorylation activity, cannot be substituted by Mg2+, Ca2+, or Zn2+
additional information
-, Q7XJ65
the expression level of the enzyme is not influenced by high salinity stress
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(4Z)-1-(3,4-dichlorophenyl)-4-(4-hydroxy-3-methoxybenzylidene)pyrazolidine-3,5-dione
-
82.6% inhibition at 0.01 mM
(4Z)-1-(3,4-dichlorophenyl)-4-(4-hydroxy-3-nitrobenzylidene)pyrazolidine-3,5-dione
-
76% inhibition at 0.01 mM
(4Z)-1-(3,4-dichlorophenyl)-4-(4-hydroxybenzylidene)pyrazolidine-3,5-dione
-
73% inhibition at 0.01 mM
(4Z)-1-(4-fluorophenyl)-4-(4-hydroxy-3-methoxybenzylidene)pyrazolidine-3,5-dione
-
44% inhibition at 0.01 mM
(4Z)-4-(4-hydroxy-3-methoxybenzylidene)-1-(4-methoxyphenyl)pyrazolidine-3,5-dione
-
55% inhibition at 0.01 mM
4-[(4Z)-4-(4-hydroxy-3-methoxybenzylidene)-3,5-dioxopyrazolidin-1-yl]benzonitrile
-
78% inhibition at 0.01 mM
genistein
-
protein tyrosine kinase inhibitor
genistein
-
competitive inhibition versus ATP, uncompetitive inhibition versus histone H1
genistein
-
-
harmine
-
potent and specific inhibitor of DYRK1A both in vitro and in cultured cells, harmine inhibits DYRK1A substrate phosphorylation more potently than it inhibits tyrosine autophosphorylation; potent and specific inhibitor of DYRK1A, inhibits DYRK1A substrate phosphorylation more potently than it inhibits tyrosine autophosphorylation
harmine
-
specific inhibitor of DYRK1A
N6-Dimethylaminopurine
-
unspecific inhibitor of protein kinases
roscovitine
-
63% inhibition at 0.01 mM
staurosporine
-
unspecific inhibitor of protein kinases
staurosporine
-
competitive versus ATP, mixed inhibition versus histone H1
tyrphostin
-
competitive versus ATP, mixed inhibition versus histone H1
Zn2+
-
inhibits the autophosphoylation, which cannot be reversed by Mn2+ addition
HCD160
-
82.6% inhibition at 0.01 mM
additional information
-
molecular docking with tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes
-
additional information
-, Q7XJ65
drought stress decreased the expression level
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
abscisic acid
Q84U74, Q8GV28, Q8GV29, Q8GV30
induces the expression of isozymes DPK1-DPK3 in seedlings; induces the expression of isozymes DPK1-DPK3 in seedlings; induces the expression of isozymes DPK1-DPK3 in seedlings
Sodium arsenite
-
the kinase activity of biliverdin reductase is stimulated by generators of free radicals such as sodium arsenite
H2O2
-
the kinase activity of biliverdin reductase is stimulated by generators of free radicals such as H2O2
additional information
-
activation of Rad53 in response to DNA damage in G(1) requires the Rad9, Mec3, Ddc1, Rad17 and Rad24 checkpoint factors, while this dependence is greatly reduced in S phase cells. Furthermore, during recovery from checkpoint activation, Rad53 activity decreases through a process that does not require protein synthesis
-
additional information
-, Q7XJ65
cold stress increased the expression level
-
additional information
-
the enzyme is induced by abiotic stresses, the activation loop is located between subdomains VII and VIII, phosphorylation at Tyr317, Tyr297, and Tyr148 activates the enzyme
-
additional information
Q84U74, Q8GV28, Q8GV29, Q8GV30
high salinity, drought, and blast by fungus Magnaporthe grisea induce the expression of isozymes DPK1-DPK3 in seedlings; high salinity, drought, and blast by fungus Magnaporthe grisea induce the expression of isozymes DPK1-DPK3 in seedlings; high salinity, drought, and blast by fungus Magnaporthe grisea induce the expression of isozymes DPK1-DPK3 in seedlings
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.019
-
ATP
-
recombinant mutant Y213F
0.034
-
ATP
-
recombinant wild-type enzyme
0.059
-
ATP
-
recombinant mutant Y317F
0.081
-
ATP
-
recombinant mutant Y297F
0.107
-
ATP
-
recombinant mutant Y148F
0.006
-
histone H1
-
recombinant mutant Y213F
-
0.007
-
histone H1
-
recombinant wild-type enzyme
-
0.009
-
histone H1
-
recombinant wild-type enzyme
-
0.011
-
histone H1
-
recombinant mutants Y148F and Y297F
-
0.012
-
histone H1
-
recombinant mutant Y317F
-
additional information
-
additional information
-
kinetics
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.26
-
ATP
-
recombinant mutant Y317F
0.54
-
ATP
-
recombinant mutant Y297F
1.02
-
ATP
-
recombinant mutant Y148F
5.25
-
ATP
-
recombinant wild-type enzyme
14.5
-
ATP
-
recombinant mutant Y213F
3.09
-
histone H1
-
recombinant mutant Y148F
-
3.3
-
histone H1
-
recombinant mutant Y317F
-
3.71
-
histone H1
-
recombinant mutant Y297F
-
4.74
-
histone H1
-
recombinant mutant Y317F
-
18.4
-
histone H1
-
recombinant mutant Y213F
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.7e-05
-
genistein
-
versus ATP, pH 7.5, 30C, recombinant mutant Y213F
2e-05
-
genistein
-
versus ATP, pH 7.5, 30C, recombinant wild-type enzyme
5.9e-05
-
genistein
-
versus ATP, pH 7.5, 30C, recombinant mutant Y317F
0.000191
-
genistein
-
versus ATP, pH 7.5, 30C, recombinant mutant Y148F
0.000194
-
genistein
-
versus ATP, pH 7.5, 30C, recombinant mutant Y297F
5.6e-05
-
staurosporine
-
pH 7.5, 30C, recombinant mutant Y213F
6.9e-05
-
staurosporine
-
versus ATP, pH 7.5, 30C, recombinant wild-type enzyme
0.000112
-
staurosporine
-
pH 7.5, 30C, recombinant mutant Y317F
0.000455
-
staurosporine
-
versus histone H1, pH 7.5, 30C, recombinant wild-type enzyme
0.000464
-
staurosporine
-
pH 7.5, 30C, recombinant mutant Y297F
0.000549
-
staurosporine
-
versus histone H1, pH 7.5, 30C, recombinant mutant Y213F
0.000571
-
staurosporine
-
pH 7.5, 30C, recombinant mutant Y148F
0.000599
-
staurosporine
-
versus histone H1, pH 7.5, 30C, recombinant mutant Y317F
0.000621
-
staurosporine
-
versus histone H1, pH 7.5, 30C, recombinant mutant Y148F
0.000694
-
staurosporine
-
versus histone H1, pH 7.5, 30C, recombinant mutant Y297F
1.4e-05
-
tyrphostin
-
versus ATP, pH 7.5, 30C, recombinant mutant Y213F
1.8e-05
-
tyrphostin
-
versus ATP, pH 7.5, 30C, recombinant wild-type enzyme
5.5e-05
-
tyrphostin
-
versus ATP, pH 7.5, 30C, recombinant mutant Y317F
0.000186
-
tyrphostin
-
versus ATP, pH 7.5, 30C, recombinant mutant Y297F
0.000187
-
tyrphostin
-
versus ATP, pH 7.5, 30C, recombinant mutant Y148F
0.000202
-
tyrphostin
-
versus histone H1, pH 7.5, 30C, recombinant wild-type enzyme
0.000426
-
genistein
-
versus histone H1, pH 7.5, 30C, recombinant wild-type enzyme
additional information
-
additional information
-
inhibition kinetics of tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0025
-
(4Z)-1-(3,4-dichlorophenyl)-4-(4-hydroxy-3-methoxybenzylidene)pyrazolidine-3,5-dione
-
for autophosphorylation
0.0006
-
(4Z)-1-(3,4-dichlorophenyl)-4-(4-hydroxy-3-nitrobenzylidene)pyrazolidine-3,5-dione
-
for autophosphorylation
0.0025
-
(4Z)-1-(3,4-dichlorophenyl)-4-(4-hydroxybenzylidene)pyrazolidine-3,5-dione
-
for autophosphorylation
0.0025
-
(4Z)-1-(4-fluorophenyl)-4-(4-hydroxy-3-methoxybenzylidene)pyrazolidine-3,5-dione
-
for autophosphorylation
0.0012
-
(4Z)-4-(4-hydroxy-3-methoxybenzylidene)-1-(4-methoxyphenyl)pyrazolidine-3,5-dione
-
for autophosphorylation
0.0006
-
4-[(4Z)-4-(4-hydroxy-3-methoxybenzylidene)-3,5-dioxopyrazolidin-1-yl]benzonitrile
-
for autophosphorylation
3.3e-05
-
harmine
-
in 25 mM HEPES, pH 7.0, 5 mM MgCl2, 0.5 mmM dithiothreitol, at 30C; isoform DYRK1A
0.000166
-
harmine
-
isoform DYRK1B
0.001
-
harmine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview
additional information
-
Q13627
presence of an extra copy of the Dyrk1A gene due to trisomy 21 results in overexpression of Dyrk1A and elevated kinase activity in brain of Down syndrome patients, tau phosphorylation, enzyme kinetics, and Dyrk1A kinase assays, nuclear and cytoplasmic Dyrk1A may contribute to Down syndrome via different mechanisms
additional information
-
-
stabilization of viral oncoproteins E6 and E7 of HPV16 by phosphorylation the threonine 5 and threonine 7 residues, substitution mutations, in vitro and in vivo interaction with HPV16E7, half-life of E7 protein extended by replacing threonine 5 and 7 residues with aspartic acid, DYRK1A-induced phosphorylation protects E7 from degradation, as a consequence inactivation of the tumor suppressor pRb is increased
additional information
-
Q13627
imbalance of 3R-tau and 4R-tau proteins attributed to Dyrk1A-induced dysregulation of alternative splicing factor (ASF), Dyrk1A phosphorylates alternative splicing factor (ASF) at Ser-227, Ser-234, and Ser-238, Dyrk1A inhibits the role of alternative splicing factor (ASF) in tau exon 10 inclusion, splicing of exon 10 of tau protein quantified, interaction between alternative splicing factor (ASF) and Dyrk1A, model for the inhibitory role of Dyrk1A in tau exon 10 inclusion via phosphorylation of ASF
additional information
-
Q922Y0
increased reticulocyte production in DYRK3-knockout mice in response to hemolytic anemia due to DYRK3 deficiency, elevated reticulocyte and red cell formation in DYRK3-knockout mice after 5-fluorouracil-induced anemia, short term transplant experiments, DYRK3-knockout progenitors support enhanced erythroblast formation, erythropoietic advantages due to DYRK3-deficiency observed in 5-fluorouracil-treated mice expressing a compromised erythropoietin receptor EPOR-HM allele, transgenic pA2gata1-DYRK3 mice produce fewer reticulocytes during hemolytic anemia, pA2gata1-DYRK3 progenitors compromised in late pro-erythroblast formation ex vivo, DYRK3 inhibits transcriptional response pathways mediated by nuclear factor of activated T cells (NFAT) in erythroid K562 cells
additional information
-
-
phosphorylation of the beta-amyloid (Ab) precursor protein APP at the threonine residue 668 by DYRK1A shown in vitro and in mammalian cells, overexpression of the human DYRK1A protein in transgenic mice reveals increased amounts of phophorylated APP precursor protein, increased amounts of phosphorylated APP precursor protein in brains of Down syndrome patients
additional information
-
Q61214
mice heterozygous for a Dyrk1A targeted mutation (Dyrk1A+/2) and wild-type, behavioral tests, non-searching and learning strategies in the Morris water maze test, swimming test, object recognition test, profound effect on phenotype by removal of single gene, haploinsufficiency of DYRK1A might contribute to an impairment of cognitive functions and stress coping behavior in human monosomy 21
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
assay at
7.4
-
-
assay at
7.4
-
Q9Z188
assay at
7.4
-
-
assay at
7.5
-
-
assay at
8.4
-
-
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
30
-
Q9Z188
assay at
30
-
-
assay at
30
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
overexpression in Down syndrome
Manually annotated by BRENDA team
-
fetal and adult
Manually annotated by BRENDA team
-
expressed in the neuronal regions affected in Down syndrome
Manually annotated by BRENDA team
-
expression pattern in frontal brain nuclei during murine embryogenesis
Manually annotated by BRENDA team
Q13627
expression analysis in
Manually annotated by BRENDA team
-
expressed in
Manually annotated by BRENDA team
-
transfection analysis
Manually annotated by BRENDA team
-
most malignant tumors assessed express TTK mRNA, as well, all rapidly proliferating cell lines tested express TTK mRNA
Manually annotated by BRENDA team
P35761
embryonal carcinoma cell line
Manually annotated by BRENDA team
Q922Y0
DYRK3 expression in, ex vivo analysis of erythroid progenitor cells of DYRK3-knockout mice
Manually annotated by BRENDA team
Q13627
co-transfection studies in
Manually annotated by BRENDA team
-
transfection analysis
Manually annotated by BRENDA team
Q13627
co-localization study in
Manually annotated by BRENDA team
-, Q7XJ65
low expression level
Manually annotated by BRENDA team
Q84U74, Q8GV28, Q8GV29, Q8GV30
high expression of DPK1; low expression of DPK4; moderate expression of DPK2; moderate expression of DPK3
Manually annotated by BRENDA team
Q9Y463
predominately expressed in muscle and testis
Manually annotated by BRENDA team
-
transfection analysis
Manually annotated by BRENDA team
Q84U74, Q8GV28, Q8GV29, Q8GV30
low expression of DPK4; moderate expression of DPK3
Manually annotated by BRENDA team
Q84U74, Q8GV28, Q8GV29, Q8GV30
-
Manually annotated by BRENDA team
Q13627
co-expression experiments in
Manually annotated by BRENDA team
Q84U74, Q8GV28, Q8GV29, Q8GV30
high expression of DPK1 in immature spikes; low expression of DPK4
Manually annotated by BRENDA team
Q84U74, Q8GV28, Q8GV29, Q8GV30
low expression of DPK2; low expression of DPK4
Manually annotated by BRENDA team
Q92630
; predominately expressed in testis
Manually annotated by BRENDA team
Q63117
predominately expressed in testis
Manually annotated by BRENDA team
Q9Y463
predominately expressed in muscle and testis
Manually annotated by BRENDA team
Q922Y0
DYRK3 expression in
Manually annotated by BRENDA team
-
transfection analysis, degradation of the tumor suppressor pRb in a dose-dependent manner based on the accumulation of HPV16E7 generated by DYRK1A transfection
Manually annotated by BRENDA team
additional information
Q9Z188
the enzyme is expressed in most tissues
Manually annotated by BRENDA team
additional information
Q84U74, Q8GV28, Q8GV29, Q8GV30
tissue-specific expression of isozymes; tissue-specific expression of isozymes; tissue-specific expression of isozymes; tissue-specific expression of isozymes
Manually annotated by BRENDA team
additional information
-
MDA-MB-435S cells show no detectable levels of STYK1/NOK
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
enzyme contains a nuclear targeting signal sequence
Manually annotated by BRENDA team
additional information
Q92630
nuclear localization of DYRK1A is mediated by its nuclear targeting signal, amino acids 105-139, but ist characteristic subnuclear distribution depends on additional N-terminal elements, amino acids 1-104
-
Manually annotated by BRENDA team
additional information
-
STY protein contains a putative nuclear localization signal
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55000
-
-
recombinant His-tagged wild-type enzyme, gel filtration
90000
-
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Q9Z188
x * 65000, splicing variant 1, SDS-PAGE, x * 70000, splicing variant 2, SDS-PAGE, x * 75000, splicing variant 3 from skeletal muscle, SDS-PAGE
additional information
-
structure modeling of wild-type and mutant enzymes based on the X-ray structure of homologous sequences, computational docking and molecular dynamics simulations, overview
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains an universal stress induced protein associated domain, USPA, and a C-terminal GUN4 domain, the latter binds magnesium-protoporphyrin IX
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains an universal stress induced protein associated domain, USPA
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains a C-terminal NirV domain and a TPR domain, an N-terminal protein kinase domain, overview
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains WD repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains N-terminal TPR repeats, followed by a GGDEF domain at the C-terminus, overview
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains PQQ family repeats, which form beta-propeller-like structures and can serve as sites for the interaction with target proteins, the enzyme also contains three to six NHL repeats, following the kinase domain, which are known to serve as protein interaction surfaces and might possess enzymic activity
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains a O-sialoglycoprotein endopeptidase, OSGP, domain at the N-terminus, the enzyme contains an N-terminal ABC1 kinase domain and a putative transmembrane spanning segment at the C-terminus
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains an universal stress induced protein associated domain, USPA
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains a O-sialoglycoprotein endopeptidase, OSGP, domain at the N-terminus
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains an N-terminal ABC1 kinase domain and a putative transmembrane spanning segment at the C-terminus
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains a O-sialoglycoprotein endopeptidase, OSGP, domain at the N-terminus
additional information
-
enzyme domain determination and analysis, unique modular organization, the enzyme contains an N-terminal protein kinase domain, a central transmembrane domain, and a C-terminal peptidyl-prolyl cis-trans isomerase domain
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains an N-terminal protein kinase domain and a hydrophobin-like region at the C-terminus, the latter plays a role in the recognition of D-alanyl-D-alanine dipeptides
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats, and three to six NHL repeats, following the kinase domain, which are known to serve as protein interaction surfaces and might possess enzymic activity, the enzyme also contains an N-terminal protein kinase domain and a hydrophobin-like region at the C-terminus, the latter plays a role in the recognition of D-alanyl D-alanine dipeptides
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains an universal stress induced protein associated domain, USPA, the enzyme contains an N-terminal ABC1 kinase domain and a putative transmembrane spanning segment at the C-terminus
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains WD repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats and a C-terminal GUN4 domain, the latter binds magnesium-protoporphyrin IX, the enzyme of strain PCC 7120 contains a pectinesterase domain catalyzing the hydrolysis of pectin, and WD repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains a PP2C-like domain at the N-terminus, and 11 STYK-like sequences, overview
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains a PP2C-like domain at the N-terminus
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats, the enzyme contains PQQ family repeats, which form beta-propeller-like structures and can serve as sites for the interaction with target proteins
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains PQQ family repeats, which form beta-propeller-like structures and can serve as sites for the interaction with target proteins
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats, the enzyme contains an N-terminal ABC1 kinase domain and a putative transmembrane spanning segment at the C-terminus
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains WD repeats
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains a O-sialoglycoprotein endopeptidase, OSGP, domain at the N-terminus, the enzyme contains an N-terminal ABC1 kinase domain and a putative transmembrane spanning segment at the C-terminus
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains an N-terminal ABC1 kinase domain and a putative transmembrane spanning segment at the C-terminus
additional information
Thermospora curvatum, Trichodesmium erythraeum
-
enzyme domain determination and analysis, modular organization, the enzyme contains WD repeats
additional information
-
analysis of sequence motifs
additional information
Mycobacterium bovis AF2
-
enzyme domain determination and analysis, modular organization, the enzyme contains an N-terminal protein kinase domain and a hydrophobin-like region at the C-terminus, the latter plays a role in the recognition of D-alanyl-D-alanine dipeptides
-
additional information
-
enzyme domain determination and analysis, modular organization, the enzyme contains several tetratrico peptide repeats, and three to six NHL repeats, following the kinase domain, which are known to serve as protein interaction surfaces and might possess enzymic activity, the enzyme also contains an N-terminal protein kinase domain and a hydrophobin-like region at the C-terminus, the latter plays a role in the recognition of D-alanyl D-alanine dipeptides
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphoprotein
-
the enzyme performs autophosphorylation, mechanism and phosphorylation sites, overview
phosphoprotein
-
MALDI-MS phosphorylation site mapping, the enzyme performs autophosphorylation within the ATP binding site at Tyr148 and Tyr317, at Tyr213 of the activation loop, and at Tyr297 in the C-terminus
phosphoprotein
-
autophosphorylated on serine and threonine residues
phosphoprotein
-
autophosphorylation
glycoprotein
-
the enzyme contains a O-sialoglycoprotein endopeptidase, OSGP, domain at the N-terminus, and is thus probably sialo-glycosylated
phosphoprotein
Q92630
autophosphorylation
phosphoprotein
-
the enzyme autophosphorylates at Thr202, Tyr72 and Tyr83, the insulin-activated insulin receptor kinase phosphorylates Y198 in the YMKM motif, Y228 in the YLSF motif, and Y291
glycoprotein
-
the enzyme contains a O-sialoglycoprotein endopeptidase, OSGP, domain at the N-terminus, and is thus probably sialo-glycosylated
phosphoprotein
O35491, O35492, O35493
autophosphorylation; autophosphorylation; autophosphorylation
phosphoprotein
P35761
autophosphorylation on serine, threonine, and tyrosine residues
phosphoprotein
-
autophosphorylation
phosphoprotein
-
autophosphorylation; dual specificity protein kinase that is regulated by tyrosine phosphorylation in the activation loop
phosphoprotein
-
autophosphorylation
phosphoprotein
-
the enzyme performs autophosporylation
glycoprotein
-
the enzyme contains a O-sialoglycoprotein endopeptidase, OSGP, domain at the N-terminus, and is thus probably sialo-glycosylated
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
7.4
-
the kinase is resistant to high salt and Triton X-100 extraction at pH 6.5. At pH 7.4, the kinase becomes soluble to some extent after treatment with Triton X-100 and 1.0 M NaCl
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Triton X-100
-
the kinase is resistant to high salt and Triton X-100 extraction at pH 6.5. At pH 7.4, the kinase becomes soluble to some extent after treatment with Triton X-100 and 1.0 M NaCl
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
gel filtration
-
gel filtration, SDS-PAGE
-
glutathione-Sepharose column chromatography
-
recombinant GST-fusion protein from Escherichia coli strain DH5alpha by glutathione affinity chromatography
-
hydroxylapatite column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sequence analysis for enzyme domain determination
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
single-copy gene, DNA and amino acid sequence determination and analysis, comparison of subdomain sequences with those of Arabidopsis thaliana
-, Q7XJ65
sequence analysis for enzyme domain determination
-
;
P49761
DNA and amino acid sequence determination and analysis, the enzyme occurs in several splicing variants, expression in human fibroblast 3T3-L1 cell line
-
expressed in Escherichia coli BL21(DE3) cells; the catalytic domain of DYRK1A is expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) codon plus RIL, plasmid pcDNA3.1
-
expressed in Mus musculus
-
expression in Escherichia coli strain DH5alpha as GST-fusion protein, expression in 293A cells
-
green fluorescent protein fusion protein of DYRK1B is found mainly in the nucleus of transfected COS-7 cells
Q9Y463
recombinant Dyrk1A-protein expressed in mammalian expression vector pcDNA3, wild-type and mutant
Q13627
transfected into NIH-3T3 cells; wild-type and mutant DNA fragments of DYRK1A, vectors pCMV-Tag2B, pRK5, pCITE4, pGEX-5X-1, pCDNA3-HA
-
DNA and amino acid sequence determination and analysis, the enzyme occurs in several splicing variants, analysis overview, expression in murine hypothalamic GT1-7 cell line, expression of splicing variants in COS_7 cells and in Escherichia coli
Q9Z188
EGFP-tagged DYRK1A is expressed in HEK-293 cell
-
genomic clones of DYRK3 isolated from a 129svJ Lambda phage library, transgenic mice generated, DYRK3 gene deletion in E14 ES cells using a floxed PGKneo targeting vector, targeted cells injected into C57BL/6J blastocysts, transgenic pA2gata1-DYRK3 mice prepared using an Myc epitope-tagged mDYRK3 cDNA within a pA2gata1 vector
Q922Y0
isozyme DPK1, DNA and amino acid sequence determination and analysis, gene mapping, expression analysis under stress conditions; isozyme DPK2, DNA and amino acid sequence determination and analysis, gene mapping, expression analysis under stress conditions; isozyme DPK3, DNA and amino acid sequence determination and analysis, gene mapping, expression analysis under stress conditions; isozyme DPK4, DNA and amino acid sequence determination and analysis, gene mapping, expression analysis under stress conditions
Q84U74, Q8GV28, Q8GV29, Q8GV30
sequence analysis for enzyme domain determination
-
fusion protein of DYRK1A accumulates in the nucleus of transfected COS-7 and HEK293 cells, expression in Escherichia coli
-
phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
there are higher levels of full-length DYRK1A in the brains of patients with Down syndrome when compared to control brains
-
Dyrk1A expression can be reressed by the AP4- geminin complex
-
Dyrk1A expression can be enhanced by treatment with beta-amyloid or overexpression of the transcription factor E2F1, Dyrk1A activity can be increased by binding of 14-3-3 protein
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
K160R
-
site-directed mutagenesis, inactive mutant enzyme
Y148F
-
site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme
Y148F
-
site-directed mutagenesis, ATP binding site residue, the mutant is unable to phosphorylate histone and is not phosphorylated itself
Y213F
-
site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme
Y213F
-
site-directed mutagenesis, TEY motif residue, the mutant shows 4fold increased autophosphorylation and 2.8fold increased activity with ATP and histone compared to the wild-type enzyme
Y297F
-
site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme
Y297F
-
site-directed mutagenesis, inactive mutant enzyme
Y317F
-
site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme
Y317F
-
site-directed mutagenesis, ATP binding site residue, the mutant is unable to phosphorylate histone and is not phosphorylated itself
K188R
-
kinase-dead DYRK1 mutant
K188R
Q13627
kinase-dead Dyrk1A construct
additional information
-
enzyme knock-out leads to increased D-glucose uptake in response to insulin
additional information
-
exchange of two Tyr residues in the activation loop between subdomains VII and VIII for Phe almost completely suppresses the activity and Tyr autophosphorylation of Dyrk. Tyr autophosphorylation is also reduced by exchange of Tyr219 in a tyrosine phosphorylation consensus motif
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
Q13627
molecular mechanism by which neurofibrillary degeneration occurs in adults with Down syndrome
medicine
-
phosphorylation and regulation of the oncogene E7 of human papillomvirus HPV16
medicine
Q13627
involvement of early onset tauopathy in individuals with Down syndrome, research and development of therapeutics for tauopathies
medicine
-
potential link between Down syndrome and Alzheimers disease by activity of DYRK1A, overexpression of DYRK1A in patients with Down syndrome may play a role in accelerating pathogenesis of Alzheimers disease through phosphorylation of the beta-amyloid (Ab) precursor protein APP, therapeutics that inhibit DYRK1A expression and/or kinase activity suggested as a possibility to suppress the early-onset of Alzheimers disease and mental retardation in Down syndrome patients
medicine
-
expression of DYRK2 can be a favorable prognostic marker in pulmonary adenocarcinoma
medicine
Q922Y0
role of DYRK3 as an erythropoietic suppressor selectively during stress erythropoiesis, possible candidate for targeting by small molecule inhibitors as potentially important anti-anemia agents
analysis
-
development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview