Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | autophosphorylation of a tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity | Homo sapiens | |
additional information | autophosphorylation of a tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity | Mus musculus |
Cloned (Comment) | Organism |
---|---|
gene DYRK4, DNA and amino acid sequence determination and analysis, splicing variants analysis and comparison to the human splicing variants, expression analysis, overview. Recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS and of the GFP-tagged DYRK4 N-terminus in HEK-293T cells | Mus musculus |
gene DYRK4, DNA and amino acid sequence determination and analysis, splicing variants analysis and comparison to the murine splicing variants, expression analysis, overview. Recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS and in insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y246F | site-directed mutagenesis, mutation of the autophosphorylation site. The DYRK4 mutant is neither autophosphorylated nor does it display kinase activity on a synthetic substrate, the exogenous peptide substrate DYRKtide | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | most of the DYRK4 isoforms show a preferential cytoplasmic localization | Homo sapiens | 5737 | - |
cytoplasm | most of the DYRK4 isoforms show a preferential cytoplasmic localization | Mus musculus | 5737 | - |
nucleoplasm | one of several splice variants of DYRK4 contains a nuclear localization signal in its extended N-terminus that mediates its interaction with importin alpha3 and alpha5 and that is capable of targeting a heterologous protein to the nucleus. The nucleocytoplasmic mobility of this variant differs from that of a shorter isoform. The long isoform (mDYRK4642) does not exit the nucleus | Mus musculus | 5654 | - |
nucleoplasm | one of several splice variants of DYRK4, the long isoform, contains a nuclear localization signal in its extended N-terminus that mediates its interaction with importin alpha3 and alpha5 and that is capable of targeting a heterologous protein to the nucleus. The nucleocytoplasmic mobility of this variant differs from that of a shorter isoform | Homo sapiens | 5654 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Homo sapiens | - |
ADP + a phosphoprotein | - |
? | |
ATP + a protein | Mus musculus | - |
ADP + a phosphoprotein | - |
? | |
additional information | Mus musculus | autophosphorylation of a tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity | ? | - |
? | |
additional information | Homo sapiens | autophosphorylation of the tyrosine 246 residue within the activation loop is necessary for full DYRK4 kinase activity, no differences in tyrosine phosphorylation are evident for the long and the short human DYRK4 isoforms | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NR20 | - |
- |
Mus musculus | Q8BI55 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation of a tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity | Homo sapiens |
phosphoprotein | autophosphorylation of a tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | several splice variants of DYRK4 are expressed in tissue-specific patterns, Dyrk4 shows a wide tissue distribution in humans | Homo sapiens | - |
additional information | several splice variants of DYRK4 are expressed in tissue-specific patterns, Dyrk4 shows a wide tissue distribution in humans | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Homo sapiens | ADP + a phosphoprotein | - |
? | |
ATP + a protein | - |
Mus musculus | ADP + a phosphoprotein | - |
? | |
ATP + biotin-Ttds-TPGSRSRTPSLPT | i.e. peptide PEP3 | Homo sapiens | ADP + phosphorylated biotin-Ttds-TPGSRSRTPSLPT | - |
? | |
ATP + biotin-Ttds-TPGSRSRTPSLPT | i.e. peptide PEP3 | Mus musculus | ADP + phosphorylated biotin-Ttds-TPGSRSRTPSLPT | - |
? | |
ATP + biotin-Ttds-VGLLKLASPELER | i.e. peptide PEP285 | Homo sapiens | ADP + phosphorylated biotin-Ttds-VGLLKLASPELER | - |
? | |
ATP + biotin-Ttds-VGLLKLASPELER | i.e. peptide PEP285 | Mus musculus | ADP + phosphorylated biotin-Ttds-VGLLKLASPELER | - |
? | |
ATP + RRARKLTATPTPLGG | i.e. peptide SAPtide | Homo sapiens | ADP + RRARKLTApTPTPLGG | - |
? | |
ATP + RRARKLTATPTPLGG | i.e. peptide SAPtide | Mus musculus | ADP + RRARKLTApTPTPLGG | - |
? | |
ATP + RRRFRPASPLRGPPK | i.e. peptide DYRKtide, designed on the basis of the phosphorylation consensus sequence for DYRK1A | Homo sapiens | ADP + RRRFRPApSPLRGPPK | - |
? | |
ATP + RRRFRPASPLRGPPK | i.e. peptide DYRKtide, designed on the basis of the phosphorylation consensus sequence for DYRK1A | Mus musculus | ADP + RRRFRPApSPLRGPPK | - |
? | |
additional information | autophosphorylation of a tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity | Mus musculus | ? | - |
? | |
additional information | autophosphorylation of the tyrosine 246 residue within the activation loop is necessary for full DYRK4 kinase activity, no differences in tyrosine phosphorylation are evident for the long and the short human DYRK4 isoforms | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dual specificity tyrosine phosphorylation-regulated kinase 4 | - |
Homo sapiens |
dual specificity tyrosine phosphorylation-regulated kinase 4 | - |
Mus musculus |
DYRK4 | - |
Homo sapiens |
DYRK4 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, recombinant enzyme | Homo sapiens |
30 | - |
assay at, recombinant enzyme | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at, recombinant enzyme | Homo sapiens |
7.4 | - |
assay at, recombinant enzyme | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme DYRK4 belongs to the dual specificity tyrosine phosphorylation-regulated kinases, DYRKs, a family of conserved protein kinases that play key roles in the regulation of cell differentiation, proliferation, and survival. DYRKs contain a conserved Tyr-Xaa-Tyr motif in the activation loop. Autophosphorylation of a second tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity and is a defining feature of the DYRK family. Family members DYRK1A, DYRK2, and DYRK4 differ in their target recognition sequence, and preference for an arginine residue at position P -3 is a feature of DYRK1A but not of DYRK2 and DYRK4 | Homo sapiens |
evolution | enzyme DYRK4 belongs to the dual specificity tyrosine phosphorylation-regulated kinases, DYRKs, a family of conserved protein kinases that play key roles in the regulation of cell differentiation, proliferation, and survival. DYRKs contain a conserved Tyr-Xaa-Tyr motif in the activation loop. Autophosphorylation of a second tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity and is a defining feature of the DYRK family. Family members DYRK1A, DYRK2, and DYRK4 differ in their target recognition sequence, and preference for an arginine residue at position P -3 is a feature of DYRK1A but not of DYRK2 and DYRK4 | Mus musculus |
malfunction | some splicing events affect the catalytic domain, including a three-amino acid deletion within subdomain XI that markedly reduces the enzymatic activity of DYRK4 | Homo sapiens |
malfunction | some splicing events affect the catalytic domain, including a three-amino acid deletion within subdomain XI that markedly reduces the enzymatic activity of DYRK4 | Mus musculus |
additional information | both human and murine DYRK4 genes express several transcripts as a result of alternative splicing, with the two main protein products differing in their N-terminal domain | Homo sapiens |
additional information | both human and murine DYRK4 genes express several transcripts as a result of alternative splicing, with the two main protein products differing in their N-terminal domain | Mus musculus |
physiological function | several splice variants of DYRK4 have distinct functional capacities | Homo sapiens |
physiological function | several splice variants of DYRK4 have distinct functional capacities | Mus musculus |