2.7.1.68: 1-phosphatidylinositol-4-phosphate 5-kinase
This is an abbreviated version!
For detailed information about 1-phosphatidylinositol-4-phosphate 5-kinase, go to the full flat file.
Word Map on EC 2.7.1.68
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2.7.1.68
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4,5-bisphosphate
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phosphoinositide
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fracture
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pip2
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actin
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femoral
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phospholipase
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ptdins4,5p2
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phospholipid
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cytoskeleton
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dislocation
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phosphatidylinositol-4,5-bisphosphate
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phosphatidic
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posterior
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gtpases
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diacylglycerol
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4-kinase
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polyphosphoinositides
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acetabular
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accident
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ossification
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talin
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avascular
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rac1
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fracture-dislocation
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bisphosphate
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screw
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arf6
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5-phosphatase
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heterotopic
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arthroplasty
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3,4,5-trisphosphate
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ptdins5p
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trochanteric
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ovcar-5
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osteonecrosis
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inositide
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osteotomy
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kinase-dead
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osteochondral
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tiazofurin
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3hinositol
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insps
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merle
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ptdins3,4,5p3
- 2.7.1.68
- 4,5-bisphosphate
- phosphoinositide
- fracture
- pip2
- actin
- femoral
- phospholipase
-
ptdins4,5p2
- phospholipid
- cytoskeleton
- dislocation
- phosphatidylinositol-4,5-bisphosphate
-
phosphatidic
-
posterior
- gtpases
- diacylglycerol
-
4-kinase
-
polyphosphoinositides
-
acetabular
-
accident
-
ossification
- talin
-
avascular
- rac1
-
fracture-dislocation
- bisphosphate
-
screw
- arf6
-
5-phosphatase
-
heterotopic
-
arthroplasty
- 3,4,5-trisphosphate
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ptdins5p
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trochanteric
-
ovcar-5
- osteonecrosis
-
inositide
-
osteotomy
-
kinase-dead
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osteochondral
- tiazofurin
-
3hinositol
-
insps
-
merle
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ptdins3,4,5p3
Reaction
Synonyms
Diphosphoinositide kinase, More, Mss4, MSS4p, phosphatidylinositol 4,5-biphosphate kinase, phosphatidylinositol 4-phosphate 5-kinase, phosphatidylinositol 4-phosphate 5-kinase 2, phosphatidylinositol 4-phosphate 5-kinase alpha, phosphatidylinositol 4-phosphate 5-kinase beta, phosphatidylinositol 4-phosphate 5-kinase gamma, phosphatidylinositol 4-phosphate 5-kinase gamma661, phosphatidylinositol 4-phosphate 5-kinase Igamma, phosphatidylinositol 4-phosphate 5-kinase Igamma b, phosphatidylinositol 4-phosphate 5-kinase Igamma_i2, phosphatidylinositol 4-phosphate 5-kinase Igamma_v3, phosphatidylinositol 4-phosphate 5-kinase Igamma_v6, phosphatidylinositol 4-phosphate 5-kinase-Ibeta, phosphatidylinositol 4-phosphate kinase, phosphatidylinositol 5-kinase gamma, phosphatidylinositol kinase type I, phosphatidylinositol monophosphate 5-kinase, phosphatidylinositol phosphate 5-kinase, phosphatidylinositol phosphate 5-kinase 1, phosphatidylinositol phosphate 5-kinase 10, phosphatidylinositol phosphate 5-kinase Ibeta, phosphatidylinositol phosphate 5-kinase Igammai2, phosphatidylinositol phosphate kinase type I gamma, phosphatidylinositol-4-phosphate 5' kinase, phosphatidylinositol-4-phosphate 5-kinase, phosphatidylinositol-4-phosphate 5-kinase gamma 635, phosphatidylinositol-4-phosphate 5-kinase1alpha, phosphatidylinositol4-phosphate 5-kinase, phosphoinositol-4-phosphate-5-kinase, PI 4-phosphate 5-kinase, PI(4)P 5-kinase, PI(4)P5-K, PI4P 5-kinase, PI4P5K, PIP kinase, PIP-5kin, PIP5K, PIP5K type I, PIP5K type Ialpha, PIP5K-Ib, PIP5K1, PIP5K1A, PIP5K1alpha, PIP5K1gamma, PIP5K2, PIP5K3, PIP5K9, PIP5Kalpha, PIP5Kbeta, PIP5Kgamma, PIP5Kgamma635, PIP5Kgamma661, PIP5KI, PIP5KI gamma, PIP5KIalpha, PIP5KIbeta, PIP5KIgamma, PIP5KIgamma_i2, PIP5KIgamma_v3, PIP5KIgamma_v6, PIP5Ks, PIPK, PIPK1, PIPK10, PIPK1gamma-87, PIPKalpha, PIPKI, PIPKIalpha, PIPKIgamma, PIPKIgammaI2, PIPKIgammai5, PIPkin, PIPkin Igammab, PIPkinIgamma93, ppk-1, PtdIns(4)P 5-kinase, PtdIns(4)P kinase, PtdIns4P5K, PtdInsP, type 1 phosphatidylinositol-4-phosphate 5-kinase, type I gamma phosphatidylinositol phosphate 5-kinase i5, type I phosphatidylinositol 4-phosphate 5-kinase, type I phosphatidylinositol 4-phosphate 5-kinase gamma, type I phosphatidylinositol phosphate 5-kinase, type I phosphatidylinositol-4-phosphate 5-kinase, type I phosphatidylinositol-4-phosphate 5-kinase alpha, type I phosphatidylinositol-4-phosphate 5-kinase gamma, type I PI 4-phosphate 5-kinase, type I PtdInsP kinase, type Igamma phosphatidylinositol 4-phosphate 5-kinase, type Igamma PIP 5-kinase
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2.7.1.68 1-phosphatidylinositol-4-phosphate 5-kinaseAdvanced search results
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results (Engineering
Engineering on EC 2.7.1.68 - 1-phosphatidylinositol-4-phosphate 5-kinase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D309N
D203A
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catalytically inactive. Expression in Xenopus laevis oocyte has no effect on epithelial sodium channel activity
D227A
D253A
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mutant lacking kinase activity. Overexpression compromises uropod formation and rear retraction during chemotaxis
D266A
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site-directed mtagenesis, mutation of the putative ATP and 1-phosphatidyl-1D-myo-inositol 4-phosphate binding site, cells form extremely extended bipolar neurits even in presence of serum, failed to interfere with cell flattening and neurite extension
D309N
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greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate
D309N/R427Q
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greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate
D322A
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isozyme PIP5Kalpha mutant that shows reduced activity compared to the wild-type enzyme
K181A
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greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate
L202I
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the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme
L210I
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the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme
R427Q
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greatly reduced kinase activity towards 1-phosphatidyl-1D-myo-inositol 4-phosphate
D316K
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site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a
K188A
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site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a
additional information
D227A
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catalytically inactive. Expression in Xenopus laevis oocyte has no effect on epithelial sodium channel activity
additional information
T-DNA insertion mutant pip5k9-d shows enhanced PIP5K9 transcript levels
additional information
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T-DNA insertion mutant pip5k9-d shows enhanced PIP5K9 transcript levels
additional information
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mutant plants homozygous for T-DNA-disrupted PIP5K3 alleles are compromised in root hair formation. Root hair-specific PIP5K3 overexpression results in root hair deformation and loss of cell polarity with increasing accumulation of PIP5K3 transcript. An N-terminal domain containing membrane occupation and recognition nexus repeats, which is not required for catalytic activity, is essential for the establishment of root hair growth. Transient heterologous expression of full-length PIP5K3 in Nicotiana tabacum pollen tubes increases plasma membrane association of a phosphatidylinositol 4,5-bisphoshate-specific reporter
additional information
overexpression of isoform PPK-1 in Caenorhabditis elegans causes an increase in 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate levels in vivo. In developing neurons, PPK-1 overexpression leads to growth cones that become stalled, produce ectopic membrane projections, and branched axons. Once neurons are established, PPK-1 overexpression results in progressive membrane overgrowth and degeneration during adulthood
additional information
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overexpression of isoform PPK-1 in Caenorhabditis elegans causes an increase in 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate levels in vivo. In developing neurons, PPK-1 overexpression leads to growth cones that become stalled, produce ectopic membrane projections, and branched axons. Once neurons are established, PPK-1 overexpression results in progressive membrane overgrowth and degeneration during adulthood
additional information
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chimeric protein in which the core kinase domain of phosphatidylinositol 4-phosphate 5-kinase 1alpha has been fused to a beta-arrestin mutant deficient in phosphatidylinositol 4,5-bisphosphate binding rescues internalization of beta2-adrenergic receptor
additional information
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knockdown of phosphatidylinositol 4-phosphate 5-kinase beta with siRNA inhibits cell polarization and impairs cell directionality during dHL60 chemotaxis
additional information
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PIP5Kgamma isozyme knockout by siRNA. Transfection of HK2 cells with PIP5Kgamma siRNA prevents advanced glycation end products-bovine serum albumin inhibition of renal Na+/K+ ATPase activity
additional information
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construction of N-terminal deletion mutants DELTA68, DELTA 72, and DELTA74 are still localized to the plasma membrane, while the N-terminal deletion mutants DELTA82, DELTA 97 are mislocated to the cytosol, construction of C-terminal deletion mutant DELTA446 is still localized to the plasma membrane, while the N-terminal deletion mutant DELTA444 is mislocated to the cytosol, mutant fusion protein DELTA68-DELTA444 localizes to the plasma membrane
additional information
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expression of phosphatidylinositol 4-phosphate 5-kinase alpha in Xenopus laevis oocytes decreases epithelial sodium channel currents by 80%, entirely due to a decrease in cell surface epithelial sodium channel levels. Expression of catalytically inactive mutants has no effect on epithelial sodium channel activity. Knock-down of phosphatidylinositol 4-phosphate 5-kinase alpha with isoform-specific siRNA results in a 4fold enhancement of epithelial sodium channel activity
additional information
construction of PIP5Kalpha knockdown BV-2 cells by shRNA
additional information
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construction of single knockout mutants of isozymes Pip5k1a and Pip5k1b, and double knockout mutants
additional information
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mutant its3-1 shows defective PIP5K activity and sensitivity to micafungin, aculeacin A, and BE49385A
additional information
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PIP5Kgamma isozyme knockout by siRNA. Transfection of HK2 cells with PIP5Kgamma siRNA prevents advanced glycation end products-bovine serum albumin inhibition of renal Na+/K+ ATPase activity
