Sequence of NCASE_PSEAE
EC Number:3.5.1.23
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
ceramidase
Q9I596
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
670
73373
Reaction
a ceramide + H2O = a carboxylate + sphingosine
Other sequences found for EC No. 3.5.1.23
General information:
Sequence
0 MSRSAFTALL LSCVLLALSM PARADDLPYR FGLGKADITG EAAEVGMMGY SSLEQKTAGI
60 HMRQWARAFV IEEAASGRRL VYVNTDLGMI FQAVHLKVLA RLKAKYPGVY DENNVMLAAT
120 HTHSGPGGFS HYAMYNLSVL GFQEKTFNAI VDGIVRSIER AQARLQPGRL FYGSGELRNA
180 NRNRSLLSHL KNPDIVGYED GIDPQMSVLS FVDANGELAG AISWFPVHST SMTNANHLIS
240 PDNKGYASYH WEHDVSRKSG FVAAFAQTNA GNLSPNLNLK PGSGPFDNEF DNTREIGLRQ
300 FAKAYEIAGQ AQEEVLGELD SRFRFVDFTR LPIRPEFTDG QPRQLCTAAI GTSLAAGSTE
360 DGPGPLGLEE GNNPFLSALG GLLTGVPPQE LVQCQAEKTI LADTGNKKPY PWTPTVLPIQ
420 MFRIGQLELL GAPAEFTVMA GVRIRRAVQA ASEAAGIRHV VFNGYANAYA SYVTTREEYA
480 AQEYEGGSTL YGPWTQAAYQ QLFVDMAVAL RERLPVETSA IAPDLSCCQM NFQTGVVADD
540 PYIGKSFGDV LQQPRESYRI GDKVTVAFVT GHPKNDLRTE KTFLEVVNIG KDGKQTPETV
600 ATDNDWDTQY RWERVGISAS KATISWSIPP GTEPGHYYIR HYGNAKNFWT QKISEIGGST
660 RSFEVLGTTP
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
64381
Okino N.,Ichinose S.,Omori A.,Imayama S.,Nakamura T.,Ito M.
Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. Cloning of a ceramidase homologue from Mycobacterium tuberculosis.
J. Biol. Chem.
274
36616-36622
1999
64382
Stover C.K.,Pham X.-Q.T.,Erwin A.L.,Mizoguchi S.D.,Warrener P.,Hickey M.J.,Brinkman F.S.L.,Hufnagle W.O.,Kowalik D.J.,Lagrou M.,Garber R.L.,Goltry L.,Tolentino E.,Westbrock-Wadman S.,Yuan Y.,Brody L.L.,Coulter S.N.,Folger K.R.,Kas A.,Larbig K.,Lim R.M.,Smith K.A.,Spencer D.H.,Wong G.K.-S.,Wu Z.,Paulsen I.T.,Reizer J.,Saier M.H. Jr.,Hancock R.E.W.,Lory S.,Olson M.V.
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.
Nature
406
959-964
2000
64383
Nieuwenhuizen W.F.,van Leeuwen S.,Jack R.W.,Egmond M.R.,Goetz F.
Molecular cloning and characterization of the alkaline ceramidase from Pseudomonas aeruginosa PA01.
Protein Expr. Purif.
30
94-104
2003
64384
Okino N.,Tani M.,Imayama S.,Ito M.
Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa.
J. Biol. Chem.
273
14368-14373
1998
64385
Kita K.,Sueyoshi N.,Okino N.,Inagaki M.,Ishida H.,Kiso M.,Imayama S.,Nakamura T.,Ito M.
Activation of bacterial ceramidase by anionic glycerophospholipids: possible involvement in ceramide hydrolysis on atopic skin by Pseudomonas ceramidase.
Biochem. J.
362
619-626
2002
64386
Tani M.,Okino N.,Sueyoshi N.,Ito M.
Conserved amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization and enzyme activity of neutral ceramidase.
J. Biol. Chem.
279
29351-29358
2004
64387
Inoue T.,Okino N.,Kakuta Y.,Hijikata A.,Okano H.,Goda H.M.,Tani M.,Sueyoshi N.,Kambayashi K.,Matsumura H.,Kai Y.,Ito M.
Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase.
J. Biol. Chem.
284
9566-9577
2009
