EC Number   |
Protein Variants |
Reference  |
|---|
   6.1.1.18 | more |
deletion mutants with C-terminal truncations and N-terminal truncations. A C-terminal deletion mutant exhibits sharp reduction in the specificity constant. Reduced stability of some of these mutants |
236 |
| 6.1.1.18 | more |
strain HAPPY101 allows plasmid-mediated expression of detrimental GlnRS mutants, which cannot complement the chromosomal glnS deletion in Escherichia coli strain X3R2 |
237 |
| 6.1.1.18 | more |
truncated enzyme form lacking the NH2-terminal domain, with modest increase in Km value for glutamine and ATP and no difference in kcat for aminoacylation or Km for tRNA |
242 |
| 6.1.1.18 | more |
3 misacylating mutant enzymes with reduced ability to discriminate between cognate and noncognate base pairs at position 3-70 |
244 |
| 6.1.1.18 | more |
temperature-sensitive mutant enzyme, no change in affinity for glutamine |
247 |
| 6.1.1.18 | more |
mutant enzyme with increased Km for glutamine |
253 |
| 6.1.1.18 | D235A |
saturation mutagenesis, only little complementation of glnS-deficient strain |
649850 |
| 6.1.1.18 | D66E |
saturation mutagenesis, 18fold increased Km for glutamine, decreased turnover |
649850 |
| 6.1.1.18 | D66F |
saturation mutagenesis, highly increased Km for glutamine, 1200fold decrease in activity |
649850 |
| 6.1.1.18 | D66G |
saturation mutagenesis, only little complementation of glnS-deficient strain |
649850 |
