EC Number   |
Protein Variants |
Reference |
|---|
   4.2.3.56 | D343A |
the mutation results in severely impaired activity for olefin production (estimated kcat reduction by more than 3000fold), the mutant produces 27.9% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 12.9% gamma-humulene (the main product of the wild type enzyme) |
713940 |
| 4.2.3.56 | D343E |
the mutant produces 34.4% sativene from (2E,6E)-farnesyl diphosphate as the main product and no gamma-humulene (the main product of the wild type enzyme), the mutant is capable of producing sesquiterpenols (61% nerolidol and 14% bisabolol, very similar to wild type distribution) at a rate comparable to that of olefin formation by the wild type enzyme |
713940 |
| 4.2.3.56 | D488A |
the mutant produces 40% sativene from (2E,6E)-farnesyl diphosphate as the main product and no gamma-humulene (the main product of the wild type enzyme) |
713940 |
| 4.2.3.56 | D488E |
the mutant produces 19.6% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 6.0% gamma-humulene (the main product of the wild type enzyme), olefin synthase activity is reduced to 3% of wild type enzyme |
713940 |
| 4.2.3.56 | D488N |
the mutant produces 21.7% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 18.7% gamma-humulene (the main product of the wild type enzyme) |
713940 |
| 4.2.3.56 | D570N |
the mutant produces 13.1% sativene from (2E,6E)-farnesyl diphosphate as the main product and 29.3% gamma-humulene (the main product of the wild type enzyme) |
713940 |
| 4.2.3.56 | Y566F |
the mutant produces 42.6% gamma-humulene (the main product of the wild type enzyme) |
713940 |
