EC Number   |
Protein Variants |
Reference |
|---|
   3.7.1.14 | C261A |
1.4fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate |
672081 |
| 3.7.1.14 | F173D |
158fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate |
672081 |
| 3.7.1.14 | F173G |
32fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate |
672081 |
| 3.7.1.14 | H114A |
MhpC mutant is able to accept the 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid, on a shorter time scale |
672080 |
| 3.7.1.14 | H114A |
shows twofold-reduced catalytic efficiency, ruling out a catalytic role, but shows a fivefold-reduced KM for the natural substrate, and an ability to process an aryl-containing substrate, implying a role for His114 in positioning of the substrate |
715899 |
| 3.7.1.14 | H263A |
mutant exhibits very slow ketonisation and C-C cleavage |
715899 |
| 3.7.1.14 | N109A |
125fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate |
672081 |
| 3.7.1.14 | N109H |
217fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate |
672081 |
| 3.7.1.14 | R188K |
217fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate |
672081 |
| 3.7.1.14 | R188Q |
2941fold decrease in kcat/Km for (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate |
672081 |
