EC Number   |
Protein Variants |
Reference |
|---|
   3.5.99.10 | E120A |
site-directed mutagenesis, the mutant has decreased deaminase activity in vitro, but retains the ability to complement ridA mutant phenotype |
-, 758515 |
| 3.5.99.10 | E180A |
site-directed mutagenesis, the mutant shows moderately reduced activity compared to wild-type |
758371 |
| 3.5.99.10 | E182A |
site-directed mutagenesis, the mutation does not significantly reduce the stability of AtRidA. The mutant shows moderately reduced activity compared to wild-type |
758371 |
| 3.5.99.10 | K136A |
site-directed mutagenesis, the mutation does not significantly reduce the stability of AtRidA. Mutant K136A severely impacts the assembly, which underscores the indispensable role of the K136-E182 salt bridge as well as the inter-subunit hydrogen bonds in maintaining the oligomeric state. The mutant shows highly reduced activity compared to wild-type |
758371 |
| 3.5.99.10 | K136R |
site-directed mutagenesis, the mutation does not significantly reduce the stability of AtRidA. The mutant shows highly reduced activity compared to wild-type |
758371 |
| 3.5.99.10 | more |
the ridA-deficient mutant of Salmonella enterica strain DM12920 can be complemented by Rid family enzymes from Pseudomonas aeruginosa strain PAO1 (Rid1 and Rid2), Acinetobacter baylyi strain ATCC 33305 (Rid2), Pseudomonas syringae pv. tomato strain ATCC BAA-871 (Rid2 and Rid3), Pseudomonas fluorescens strain ATCC BAA-477 (Rid3), and by ridA from the Salmonella enterica wild-type LT2 |
-, 758158 |
| 3.5.99.10 | R105A |
site-directed mutagenesis, inactive mutant |
-, 758515 |
| 3.5.99.10 | R165A |
site-directed mutagenesis, the mutation shows 98% reduced activity compared to wild-type. The mutant shows moderately reduced activity compared to wild-type |
758371 |
| 3.5.99.10 | S166A |
site-directed mutagenesis, the mutation does not significantly reduce the stability of AtRidA. The mutant shows moderately reduced activity compared to wild-type |
758371 |
| 3.5.99.10 | S80A |
site-directed mutagenesis, the mutation does not significantly reduce the stability of AtRidA. The mutant shows moderately reduced activity compared to wild-type |
758371 |
