EC Number   |
Protein Variants |
Reference |
|---|
    3.5.4.10 | D125A |
10fold increase in KM-value |
658026 |
| 3.5.4.10 | K137A |
30fold increase in KM-value, 30fold decrease in kcat-value |
658026 |
| 3.5.4.10 | D125N |
3fold increase in Km-value |
658026 |
| 3.5.4.10 | K137R |
3fold increase in KM-value15fold decrease in kcat-value |
658026 |
| 3.5.4.10 | D125E |
5fold increase in Km-value |
658026 |
| 3.5.4.10 | H256A |
complete loss of activity, mutation lowers the thermostability |
756701 |
| 3.5.4.10 | K255A |
complete loss of activity, mutation lowers the thermostability |
756701 |
| 3.5.4.10 | N415A |
decrease in AICAR transformylase activity as compared with wild-type SlugATIC, indicating it might also play essential role in substrate binding. Increase in the thermostability |
756701 |
| 3.5.4.10 | Y104A |
decrease in kcat-value by one orders of magnitude |
658026 |
| 3.5.4.10 | Y104F |
decrease in kcat-value by two orders of magnitude |
658026 |
