EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.133 | V329S/A330C/N331S/E332P |
A-18, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch and to maltotriose than to acarbose compared to wild-type |
699487 |
| 3.2.1.133 | V329A/A330C/N331G/E332V |
A-39, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch and to maltotriose than to acarbose compared to wild-type |
699487 |
| 3.2.1.133 | N147D/F195L/N263S/D311G/A344V/F397S/N508D/M375T |
additional exchange M375T of mutant III-2 responsible for decreased specific activity, lineage of shuffling mutants shown |
-, 679745 |
| 3.2.1.133 | V329A/N331L |
B-4, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch compared to wild-type |
699487 |
| 3.2.1.133 | A330M/N331G/E332C |
B-96, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch and to maltotriose than to acarbose compared to wild-type |
699487 |
| 3.2.1.133 | A330G/N331G/E332C |
C-20, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch and to maltotriose than to acarbose compared to wild-type |
699487 |
| 3.2.1.133 | A330G/N331P/E332G |
C-43, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch and to maltotriose than to acarbose compared to wild-type |
699487 |
| 3.2.1.133 | V329I/A330G/N331W |
C-56, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch and to maltotriose than to acarbose compared to wild-type |
699487 |
| 3.2.1.133 | V329C/N331H/E332R |
D-3, strong reduction of all substrate hydrolyzing activities, lower relative specificity to beta-cyclodextrin and pullulan than to starch and to maltotriose than to acarbose compared to wild-type |
699487 |
| 3.2.1.133 | G50I/D109E |
double mutation, two main residues of the catalytic binding pocket, site-directed mutagenesis |
678795 |
