EC Number |
Protein Variants |
Reference |
---|
3.1.1.89 | K38A |
mutation does not abolish nuclear localization of enzyme |
714919 |
3.1.1.89 | more |
construction of deletion mutants and expression in HeLa cells. Domain 1 of the enzyme extends from amino acids 1 to 233, domain 2 from amino acids 234 to 284 and domain 3 includes amino acids 285 to 378. Domain 1 of PME-1 does not migrate into the nucleus and domain 3 shows a speckled cytoplasmic distribution, whereas domain 2 is targeted to the nucleus |
714919 |
3.1.1.89 | R271A |
mutation abolishes nuclear localization of enzyme |
714919 |
3.1.1.89 | R37A |
mutation does not abolish nuclear localization of enzyme |
714919 |
3.1.1.89 | R39A |
mutation does not abolish nuclear localization of enzyme |
714919 |
3.1.1.89 | S156A |
mutation abolishes the methylesterase activity, residue S156 is a functional active site serine |
714919 |