EC Number |
Protein Variants |
Reference |
---|
2.5.1.148 | T65V/M180L |
activity of the mutant enzyme is similar to that of the wild-type enzyme |
749460 |
2.5.1.148 | V289C/S276Y |
for the V289C/S276Y double mutant, in comparison to the S276Y parent background, a small increase in C35H58 production is observed, whereas the activities for lycopaoctaene and squalene production remain the same |
749460 |
2.5.1.148 | T65V/M180L/S276Y A288F |
in comparison to the M9 parent background, the mutant enzyme shows a further reduction in C35H58 production and no significant change in lycopaoctaene and squalene formation |
749460 |
2.5.1.148 | T65V/M180L/S276Y/A288F/V289C |
in comparison to the M9 parent background, the mutant enzyme shows a further reduction in C35H58 production and no significant change in lycopaoctaene and squalene formation |
749460 |
2.5.1.148 | M180L |
mutant retains the ability to produce lycopaoctaene |
749460 |
2.5.1.148 | T65V |
mutant retains the ability to produce lycopaoctaene |
749460 |
2.5.1.148 | V289C |
mutant retains the ability to produce lycopaoctaene |
749460 |
2.5.1.148 | S276Y/A288F |
mutant shows a drastic reduction in the ability to produce lycopaoctaene and C35H58 compared to wild-type enzyme |
749460 |
2.5.1.148 | T65V/M180L/A288F |
mutant shows activity similar to that of the M4 parent background |
749460 |
2.5.1.148 | more |
mutating Ser276 and Ala288 residues to those found in human squalene synthase largely converts LOS from lycopaoctaene production to C30 squalene production |
749460 |