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Results 1 - 10 of 13 > >>
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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248V744L 2fold increase in reaction velocity, 3fold increase in Km value. Activation by Ca2+ and inactivation by Cu2+ are reduced -, 698518
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248A452N 3fold increase in reaction velocity, 9fold increase in Km value. Activation by Ca2+ and inactivation by Cu2+ are reduced -, 698518
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248more construction of CITase deletion mutants, 15 deletion mutant enzymes. M123DELTA (R4-deleted), MDELTA234 (R1-deleted), and MDELTA23DELTA (R1/R4-deleted) catalyze cycloisomaltooligosaccharide synthesis, but other mutants are inactive. M123DELTA, MDELTA234, and MDELTA23DELTA increase their Km values against dextran 40. The wild-type enzyme and M123DELTA produced cycloisomaltooligosaccharide-8 predominantly, but MDELTA234 and MDELTA23DELTA lose cycloisomaltooligosaccharide-8 production specificity. The kcat values of MDELTA234 and MDELTA23DELTA decrease, and these mutants show narrowed temperature and pH stability ranges, product size distribution of mutants, overview -, 721720
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248F268V/D469Y/A513V/Y515S mutant produces three times as much megalo-cycloisomaltooligosaccharides (10-12 glucose units) and 1.5 times as much total cycloisomaltooligosaccharides (7-12 glucose units) as compared with the wild-type. The modified product size specificity is attributable to the construction of novel substrate-binding sites in the B-2 substrate-binding site and reactivity is improved by mutation on subsite -3 on the catalytic domain -, 735639
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248D269N site-directed mutagenesis, the mutant is almost inactive 721736
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248D269A site-directed mutagenesis, the mutant is inactive 721736
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248E341A site-directed mutagenesis, the mutant is inactive 721736
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248D144A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 721736
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248D144N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 721736
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.248E341Q site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 721736
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