EC Number   |
Protein Variants |
Reference  |
|---|
    2.4.1.18 | R384A |
mutation causes almost complete inactivation |
636946 |
| 2.4.1.18 | R384E |
mutation causes almost complete inactivation |
636946 |
| 2.4.1.18 | R384K |
residual activity of the mutant enzyme is 5% of the wild-type enzyme |
636946 |
| 2.4.1.18 | R384Q |
mutation causes almost complete inactivation |
636946 |
| 2.4.1.18 | R384S |
mutation causes almost complete inactivation |
636946 |
| 2.4.1.18 | truncated enzyme form missing the first 107 amino- |
the purified full-length enzyme is poorly soluble and forms aggregates, which are inactive, at concentrations above 1 mg/ml. In contrast, the truncated form can be concentrated to 6 mg/ml without a visible signs of aggregation or loss of activity on concentration |
636953 |
| 2.4.1.18 | Y300A |
mutant enzyme shows less than 1% of the wild-type activity |
636959 |
| 2.4.1.18 | Y300D |
mutant enzyme shows less than 1% of the wild-type activity |
636959 |
| 2.4.1.18 | Y300F |
mutant enzyme shows 25% of the wild-type activity, no effect on Km-value, heat stability is lowered significantly compared to that of the wild-type enzyme, lower relative activity at elevated temperatures compared to wild-type enzyme |
636959 |
| 2.4.1.18 | Y300L |
mutant enzyme shows less than 1% of the wild-type activity |
636959 |
