EC Number   |
Protein Variants |
Reference |
|---|
  2.1.1.164 | C70A |
kcat for S-adenosyl-L-methionine is nearly identical to wild-type value |
698786 |
| 2.1.1.164 | C70S |
kcat for S-adenosyl-L-methionine is 1.53fold higher than wild-type value |
698786 |
| 2.1.1.164 | D166A |
kcat for S-adenosyl-L-methionine is 10% of wild-type value |
698786 |
| 2.1.1.164 | H140A |
kcat for S-adenosyl-L-methionine is 5% of wild-type value |
698786 |
| 2.1.1.164 | H141A |
kcat for S-adenosyl-L-methionine is 21% of wild-type value |
698786 |
| 2.1.1.164 | H149A/H141A |
activity is below the detection limit |
698786 |
| 2.1.1.164 | L136V |
kcat for S-adenosyl-L-methionine is 54% of wild-type value |
698786 |
| 2.1.1.164 | P75S |
although properly folded based upon CD spectroscopy, the mutant displays a substantially reduced affinity for AdoMet (about 10fold increase in Km). kcat for S-adenosyl-L-methionine is 13% of wild-type value |
698786 |
| 2.1.1.164 | S138A |
kcat for S-adenosyl-L-methionine is 49% of wild-type value |
698786 |
| 2.1.1.164 | W134Y |
kcat for S-adenosyl-L-methionine is 35% of wild-type value |
698786 |
