EC Number   |
Protein Variants |
Reference |
|---|
   1.8.4.14 | C118A |
inactive |
-, 725400 |
| 1.8.4.14 | C136A |
about 60% of the catalytic efficiency of wild-type |
-, 725400 |
| 1.8.4.14 | C15S |
kcat and Km values of C15S are similar to those of wild type. Cells expressing the C15S mutant enzyme show similar growth to wild type-expressing cells |
729109 |
| 1.8.4.14 | C15S |
mutant exhibits apparent activity. The kcat and Km values of the mutant enzyme are similar to those of wild type, showing that Cys15 is dispensable for catalysis |
726800 |
| 1.8.4.14 | C60S |
mutant exhibits apparent activity. The kcat value is dramatically reduced in the C60S mutant (80fold lower), while the Km value does not significantly change compared to wild type |
726800 |
| 1.8.4.14 | C60S |
the kcat value is reduced in the C60S mutant (80fold lower), while the Km value does not significantly change compared to wild type. In cells expressing C60S, growth is not observed during a 3-day incubation but observed at a 7-day incubation |
729109 |
| 1.8.4.14 | C84A |
about 120% of the catalytic efficiency of wild-type |
-, 725400 |
| 1.8.4.14 | C84A/C136A |
catalytic efficiency similar to wild-type |
-, 725400 |
| 1.8.4.14 | C84A/C94A/C136A |
about 35% of the catalytic efficiency of wild-type |
725400 |
| 1.8.4.14 | C84S |
activity is not detectable. Cells expressing C84S do not grow in the methionione sulfoxide medium at all |
729109 |
