EC Number   |
Protein Variants |
Reference |
|---|
    1.3.1.85 | A182G |
inactive |
-, 739852 |
| 1.3.1.85 | A182L |
the mutant with increased activity selectively catalyzes carboxylation over the regular reduction reaction |
-, 739852 |
| 1.3.1.85 | E171A |
the mutant shows reduced activity compared to the wild type enzyme |
-, 760072 |
| 1.3.1.85 | F170A |
in the mutant, the carboxylation activity is decreased to 17% at the expense of increased reduction side reactivity compared to the wild type enzyme |
-, 760072 |
| 1.3.1.85 | F170Y |
the mutant shows a slightly increased substrate inhibition, but otherwise very similar kinetic parameters as the wild type enzyme |
760072 |
| 1.3.1.85 | H365N |
the mutant shows a 20fold decreased activity compared with the wild type enzyme but still displays 93% of carboxylated product, even though the KM for CO2 is raised by more than 1 order of magnitude |
-, 760072 |
| 1.3.1.85 | N81L |
the interaction of the enzyme with CO2 is severely disturbed by the N81L mutation. Stereospecificity of butyryl-CoA formation is almost completely lost in the mutant compared with the wild type enzyme |
-, 760072 |
| 1.3.1.85 | S119A |
the mutant shows almost full carboxylation, but at more than 50fold reduced turnover frequency as compared to the wild type enzyme |
-, 760072 |
| 1.3.1.85 | T82D |
the mutant shows almost full carboxylation, but at more than 50fold reduced turnover frequency as compared to the wild type enzyme |
760072 |
| 1.3.1.85 | V350A |
the mutant shows increased activity compared to the wild type enzyme |
-, 739852 |
