EC Number   |
Protein Variants |
Reference |
|---|
    1.3.1.72 | E191K |
decreased activity |
690082 |
| 1.3.1.72 | E191K |
natural mutant, 20% residual enzyme activity |
654165 |
| 1.3.1.72 | E191K |
site-directed mutagenesis, mutation of a FAD binding domain residue, 19.9% of wild-type activity remain |
746354 |
| 1.3.1.72 | E480K |
site-directed mutagenesis, mutation of a C-terminal domain residue, about 50% of wild-type activity remain |
746354 |
| 1.3.1.72 | K306N |
decreased activity |
690082 |
| 1.3.1.72 | K306N |
natural mutant, 50% residual enzyme activity |
654165 |
| 1.3.1.72 | K306N |
site-directed mutagenesis, mutation of a C-terminal domain residue, 49.8% of wild-type activity remain |
746354 |
| 1.3.1.72 | more |
construction of a diminuto/dwarf1 (dim) T-DNA insertion mutant. The dim mutant plants, similarly to the dwarf5-2, show a dwarf phenotype due to the deficiency in brassinosteroids, caused by a block in the D24-sterol-D24-reductase activity. Compared to the wild-type, the dim plants show reduced levels of campesterol and sitosterol associated to an increase of 24-methylene cholesterol and isofucosterol, their respective metabolic precursors and substrates of DIM, complementation experiments with dwarf5-2, dim, and ste1-1 |
-, 746241 |
| 1.3.1.72 | more |
construction of a specific chimeric antibody 2-152a MAb |
746181 |
| 1.3.1.72 | more |
construction of a truncated DHCR24, DELTA23 DHCR24, lacking the secretory signal peptide |
744530 |
