EC Number   |
Protein Variants |
Reference |
|---|
   1.14.11.9 | biotecnology |
AaF3H is a potential target for regulation of flavonoids biosynthesis in Artemisia annua through metabolic engineering |
746122 |
| 1.14.11.9 | D195E |
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol |
689577 |
| 1.14.11.9 | D195E/L215V/K216R |
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol |
689577 |
| 1.14.11.9 | D331H |
no catalytic activity |
689577 |
| 1.14.11.9 | H220Q |
catalytic activity is reduced to about 0.15% of that of the wild-type enzyme. Slightly increased Km-value with respect to iron binding, as compared to the wild-type enzyme |
439124 |
| 1.14.11.9 | H278Q |
mutant enzyme has no detectable enzyme activity |
439124 |
| 1.14.11.9 | I115T |
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol |
689577 |
| 1.14.11.9 | I115T/V116I |
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol |
689577 |
| 1.14.11.9 | I115T/V116I/I131F/D195E |
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 76% flavanone 3beta-hydroxylase product dihydrokaempferol and to 24% flavone synthase product apigenin |
689577 |
| 1.14.11.9 | I131F |
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol |
689577 |
