EC Number   |
Protein Variants |
Reference |
|---|
  1.1.1.326 | K159A |
site-directed mutagenesis, inactive mutant |
717593 |
| 1.1.1.326 | more |
de novo production of zerumbone is achieved in a metabolically engineered yeast cell factory by introducing alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1), and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana into a Saccharomyces cerevisiae strain LW14-16. The overexpression of the mevalonate (MVA) pathway rate-limiting enzymes tHMG1 and ERG20, regulation of ERG9 by an inducible promoter and competitive pathway deletion redirects the metabolic flux toward the desired product. Expressing ZSD1S114A in the ura3 site of strain LW14 results in the production of 7 mg/l zerumbone, multicopy integration of ZSD1S114A increases the production of zerumbone to 20.6 mg/l, production of zerumbone finally reached 40 mg/l by fed-batch fermentation in a 5-l bioreactor using strain LW16, method overview |
748561 |
| 1.1.1.326 | S114A |
site-directed mutagenesis, the mutation of Ser144 to Ala enhances the catalytic efficiency of ZSD1 to 10-hydroxy-alpha-humulene compared to wild-type |
748561 |
| 1.1.1.326 | S142A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
717593 |
| 1.1.1.326 | S144A |
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme |
717593 |
| 1.1.1.326 | Y155A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
717593 |
