EC Number |
Protein Variants |
Reference |
---|
3.6.1.25 | K52R |
the His-tagged mutant loses 90-99% of its activity (catalytic efficiency is at least 1000times lower) compared to the wild type enzyme. Mn2+ does not induce a significant activation of this mutant |
719965 |
3.6.1.25 | K85A |
the His-tagged mutant is about 10times less active than the wild type enzyme, but the optimal conditions for activity are essentially the same. The mutant is more strongly activated by Mn2+ than by Mg2+, and the inhibitory effects of Ca2+ and Zn2+ are less pronounced |
719965 |
3.6.1.25 | K8A |
the His-tagged mutant remains highly active with tripolyphosphate as substrate and Mg2+ as activator, with a catalytic efficiency close to that of the recombinant wild type enzyme |
719965 |
3.6.1.25 | R265H |
no AdoMet synthetase activity, normal tripolyphosphatase activity |
669193 |
3.6.1.25 | R265S |
no AdoMet synthetase activity, reduced tripolyphosphatase activity |
669193 |