EC Number   |
Protein Variants |
Reference |
|---|
   4.1.2.47 | W128A/P187L |
no activity in cleavage reaction with (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile |
691803 |
| 4.1.2.47 | W128A/Q215H |
slightly increased activity with the substrate (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile compared to the starting clone W128A |
691803 |
| 4.1.2.47 | W128C |
mutant enzyme is (S)-selective as the wild-type enzyme |
661490 |
| 4.1.2.47 | W128C |
mutation increases the specific activity towards 4-hydroxymandelonitrile of the various MeHNL mutant compared to the wild-type enzyme |
650854 |
| 4.1.2.47 | W128L |
mutant enzyme is (S)-selective as the wild-type enzyme |
661490 |
| 4.1.2.47 | W128L |
mutation increases the specific activity towards 4-hydroxymandelonitrile of the various MeHNL mutant compared to the wild-type enzyme |
650854 |
| 4.1.2.47 | W128Y |
mutant enzyme is (S)-selective as the wild-type enzyme |
661490 |
| 4.1.2.47 | W128Y |
mutation increases the specific activity towards 4-hydroxymandelonitrile of the various MeHNL mutant compared to the wild-type enzyme |
650854 |
| 4.1.2.47 | Y133F |
the mutant shows lower specific activity towards racemic mandelonitrile compared to the wild type enzyme |
747531 |
