EC Number   |
Protein Variants |
Reference |
|---|
    2.4.1.18 | Y300F |
mutant enzyme shows 25% of the wild-type activity, no effect on Km-value, heat stability is lowered significantly compared to that of the wild-type enzyme, lower relative activity at elevated temperatures compared to wild-type enzyme |
636959 |
| 2.4.1.18 | Y300L |
mutant enzyme shows less than 1% of the wild-type activity |
636959 |
| 2.4.1.18 | Y300S |
mutant enzyme shows less than 1% of the wild-type activity |
636959 |
| 2.4.1.18 | Y300W |
mutant enzyme shows less than 1% of the wild-type activity |
636959 |
| 2.4.1.18 | Y352F |
site-directed mutagenesis, , the mutant shows a much lower activity compared to wild-type enzyme mSBEIIa |
737078 |
