EC Number   |
|---|
   7.2.2.14 | both full-length enzyme and isolated cytoplasmic domain. Enzyme forms a funnel-shaped homopentamer with two transmembrane domains per monomer. An Mg2+ ion is bound between monomers at a conserved site in the cytoplasmic domain |
| 7.2.2.14 | crystal structure analysis from PDB ID BBJ2 at 3.9 A resolution for the whole enzyme, and at 1.8 A resolution for the soluble domain, overview |
| 7.2.2.14 | crystal structure analysis, X-ray structure determination at 3.9 A resolution, PDB ID 2BBJ, overview |
| 7.2.2.14 | enzyme forms a pentameric cone-shaped structure with regulatory metal binding sites in the N-terminal domain |
| 7.2.2.14 | free wild-type CorA, CorA1-351, and SeMet-AfCorA1-263, in complex with several different metal ions, 15 mg/ml purified CorA1-351 in 20 mM Tris pH 8.0, 100 mM NaCl, 1 mM TCEP, with or without 0.026% n-dodecyl-beta-D-maltoside, with precipitant vapor-diffusion over 20% PEG 400, 0.2 M CaCl2, 0.1 M HEPES, pH 7.0, 40 mg/ml native and SeMet-AfCorA1-263 from 1.3 M ammonium sulfate, 10 mM CoCl2, 0.1 M HEPES, pH 7.4, X-ray diffraction structure determination and analysis at 3.7 A resolution |
| 7.2.2.14 | periplasmic domain of recombinant enzyme |
| 7.2.2.14 | recombinant enzyme, purification omits the isolation and subsequent solubilization of the membrane fraction |
| 7.2.2.14 | selenomethionine-labeled full-length enzyme and soluble cytosolic domain in presence and absence of Mg2+, 10 mg/ml full-length protein with 0.1% dodecylmaltoside, by vapour diffusion over 32-34% 2-methyl-2,4-pentanediol, 40 mM magnesium acetate, and 100 mM MES, pH 6.0, cytosolic domain with bound Mg2+ by vapour diffusion over solutions containing 18-22% PEG 400, 0.2M MgCl2, and 0.1M HEPES, pH 7.4, or cytosolic domain in the absence of Mg2+ in 20% PEG 3350, 0.2 M ammonium acetate, and 0.5% octyl-beta-D-glucopyranoside, X-ray diffraction structure determination at 2.3-3.9 A resolution, multiple anomaleous dispersion method |
| 7.2.2.14 | single particle electron microscopy of recombinant periplasmic domain, enzyme is a pyramid-like homotetramer with a central cavity |
